Results 31 to 40 of about 6,562,675 (210)

Duplex DNA and BLM regulate gate opening by the human TopoIIIα-RMI1-RMI2 complex

Nature Communications, 2022
Here the authors probe the cleavage and gate opening of single-stranded DNA by the human topoisomerase TRR using a unique single-molecule strategy to reveal structural plasticity in response to both double-stranded DNA and the helicase BLM.
Julia A. M. Bakx, Andreas S. Biebricher, Graeme A. King, Panagiotis Christodoulis, Kata Sarlós, Anna H. Bizard, Ian D. Hickson, Gijs J. L. Wuite, Erwin J. G. Peterman   +8 more
doaj   +1 more source

New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

BioTechniques, 2015
An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples.
Nathan Wales, Christian Carøe, Marcela Sandoval-Velasco, Cristina Gamba, Ross Barnett, José Alfredo Samaniego, Jazmín Ramos Madrigal, Ludovic Orlando, M. Thomas P. Gilbert   +8 more
doaj   +1 more source

Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro. [PDF]

PLoS ONE, 2018
Prokaryotic argonautes are a unique class of nucleic acid-guided endonucleases putatively involved in cellular defense against foreign genetic elements.
Eric A Hunt, Thomas C Evans, Nathan A Tanner   +2 more
doaj   +1 more source

CNN-Pred: Prediction of single-stranded and double-stranded DNA-binding protein using convolutional neural networks

, 2023
DNA-binding proteins play a vital role in biological activity including DNA replication, DNA packing, and DNA reparation. DNA-binding proteins can be classified into single-stranded DNA-binding proteins (SSBs) or double-stranded DNA-binding proteins ...
Sharma, Ronesh, Dehzangi, Iman, Sharma, Alok, Shatabda, Swakkhar, Tsunoda, Tatsuhiko, Manavi, Farnoush   +5 more
core   +1 more source

DNA replication is required for the checkpoint response to damaged DNA in Xenopus egg extracts. [PDF]

, 2002
Alkylating agents, such as methyl methanesulfonate (MMS), damage DNA and activate the DNA damage checkpoint. Although many of the checkpoint proteins that transduce damage signals have been identified and characterized, the mechanism that senses the ...
Lindsay, Howard D., Stokes, Matthew P., Van Hatten, Ruth, Michael, W. Matthew   +3 more
core   +5 more sources

Single-stranded and double-stranded DNA-binding protein prediction using HMM profiles

, 2020
Background: DNA-binding proteins perform important roles in cellular processes and are involved in many biological activities. These proteins include crucial protein-DNA binding domains and can interact with single-stranded or double-stranded DNA, and ...
Sharma, Ronesh, Kumar, Shiu, Sharma, Alok, Kumarevel, Thirumananseri, Tsunoda, Tatsuhiko   +4 more
core   +1 more source

Identification of binding mechanisms in single molecule-DNA complexes [PDF]

, 2003
Eckel R, Ros R, Ros A, Wilking SD, Sewald N, Anselmetti D. Identification of binding mechanisms in single molecule-DNA complexes. Biophysical journal. 2003;85(3):1968-1973.Changes in the elastic properties of single deoxyribonucleic acid (DNA) molecules ...
Anselmetti, Dario, Wilking, Sven David, Ros, Robert, Eckel, Rainer, Sven David Wilking, Dario Anselmetti, Robert Ros, Wilking, S.D., Sewald, Norbert ; https://orcid.org/, Ra Ros, Ros, Alexandra, Sewald, Norbert, Rainer Eckel, Anselmetti, Dario ; https://orcid.org/, Y Norbert Sewald   +14 more
core   +1 more source

hSSB1 rapidly binds at the sites of DNA double-strand breaks and is required for the efficient recruitment of the MRN complex [PDF]

, 2010
hSSB1 is a newly discovered single-stranded DNA (ssDNA)-binding protein that is essential for efficient DNA double-strand break signalling through ATM. However, the mechanism by which hSSB1 functions to allow efficient signalling is unknown.
Cubeddu, Liza (R16726), Prise, Kevin M., Schettino, Giuseppe, So, Sairei, Bolderson, Emma, Kevin M. Prise, Derek J. Richard, Cubeddu, Liza, Kerry Richard, White, Malcolm F., Emma Bolderson, David J. Chen, Mihaela Ghita, Kienan Savage, Giuseppe Schettino, Savage, Kienan, Richard, Kerry, Khanna, Kum Kum, Ghita, Mihaela, Malcolm F. White, Sairei So, Richard, Derek J., Kum Kum Khanna, Chen, David J., Liza Cubeddu, Prise, Kevin; id_orcid, Kerry, Richard, Emma, Bolderson, Chen David, J., Kum, Khanna Kum, White Malcolm, F., Liza, Cubeddu, Richard Derek, J., Sairei, So   +33 more
core   +2 more sources

Hybrids of Nucleic Acids and Carbon Nanotubes for Nanobiotechnology

Nanomaterials, 2015
Recent progress in the combination of nucleic acids and carbon nanotubes (CNTs) has been briefly reviewed here. Since discovering the hybridization phenomenon of DNA molecules and CNTs in 2003, a large amount of fundamental and applied research has been ...
Kazuo Umemura
doaj   +1 more source

Seamless Insert-Plasmid Assembly at High Efficiency and Low Cost. [PDF]

PLoS ONE, 2016
Seamless cloning methods, such as co-transformation cloning, sequence- and ligation-independent cloning (SLIC) or the Gibson assembly, are essential tools for the precise construction of plasmids.
Roger M Benoit, Christian Ostermeier, Martin Geiser, Julia Su Zhou Li, Hans Widmer, Manfred Auer   +5 more
doaj   +1 more source