Results 71 to 80 of about 11,463 (183)

Transcription of Superhelical DNA from Cell Nuclei [PDF]

European Journal of Biochemistry, 1977
Structures resembling nuclei may be released by gently lysing human or frog cells in solutions containing non‐ionic detergents and 1.95 M NaCl. These structures, or nucleoids, sediment in sucrose gradients containing intercalating agents in the manner characteristic of DNA that is intact, supercoiled and circular.
Colman, A, Cook, P
openaire   +3 more sources

Clinical Features of Seven COL2A1 Variations in Chinese Children With Type II Collagen Disorders

Acta Paediatrica, Volume 114, Issue 7, Page 1720-1730, July 2025.
ABSTRACT Aim Type II collagen, encoded by the collagen type II alpha 1 (COL2A1) gene, is crucial for the structure of cartilage. This study aims to improve our understanding of Spondyloepiphyseal Dysplasia Congenita (SEDC) caused by mutations in COL2A1. We also aim to evaluate the safety and efficacy of growth hormone (GH) therapy in two SEDC patients.
Shumin Zhan, Qin He, Jinna Yuan, Xiaoqin Xu, Ke Huang, Guanping Dong, Junfen Fu, Dingwen Wu, Wei Wu   +8 more
wiley   +1 more source

Helping Eve Overcome ADAM: G-Quadruplexes in the ADAM-15 Promoter as New Molecular Targets for Breast Cancer Therapeutics

Molecules, 2013
ADAM-15, with known zymogen, secretase, and disintegrin activities, is a catalytically active member of the ADAM family normally expressed in early embryonic development and aberrantly expressed in various cancers, including breast, prostate and lung ...
Robert V. Brown, Vanessa C. Gaerig, Taesha Simmons, Tracy A. Brooks   +3 more
doaj   +1 more source

Conformational change-induced repeat domain expansion regulates Rap phosphatase quorum-sensing signal receptors. [PDF]

PLoS Biology, 2013
The large family of Gram-positive quorum-sensing receptors known as the RNPP proteins consists of receptors homologous to the Rap, NprR, PlcR, and PrgX proteins that are regulated by imported oligopeptide autoinducers.
Vijay Parashar, Philip D Jeffrey, Matthew B Neiditch   +2 more
doaj   +1 more source

Interplay between DNA sequence and negative superhelicity drives R-loop structures. [PDF]

, 2019
R-loops are abundant three-stranded nucleic-acid structures that form in cis during transcription. Experimental evidence suggests that R-loop formation is affected by DNA sequence and topology. However, the exact manner by which these factors interact to
Benham, Craig J, Chedin, Frederic, Hartono, Stella R, Malig, Maika, Stolz, Robert, Sulthana, Shaheen   +5 more
core  

Simian virus 40 chromatin showing nucleosomes in linear bead-like arrangements along extended closed circular DNA [PDF]

, 1977
Viral nucleoprotein complexes were extracted from nuclei of permissive cells (CV-1) infected with simian virus 40 (SV40) and examined by electron microscopy.
Hidaka, Hideyuki, Nakamura, Takashi, Oda, Takuzo, Watanabe, Sekiko   +3 more
core   +1 more source

The dissociation of nuclear proteins from superhelical DNA

Journal of Cell Science, 1978
ABSTRACT Structures retaining many of the morphological features of nuclei may be released by gently lysing human cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids contain superhelical DNA.
Levin, J, Jost, E, Cook, P
openaire   +3 more sources

A cellular system to study responses to a collision between the transcription complex and a protein‐bound nick in the DNA template

FEBS Letters, Volume 599, Issue 12, Page 1749-1759, June 2025.
We present the cellular transcription‐coupled Flp‐nick system allowing the introduction of a Top1‐mimicking cleavage complex (Flpcc) at a Flp recognition target site within a controllable LacZ gene. LacZ transcription leads to the collision of RNA polymerase II (RNAPII) with Flpcc, and this causes RNAPII stalling, ubiquitination, and degradation.
Petra Herring, Morten Roedgaard, Camilla Myrup Holst, Helene Christensen, Birgitta R. Knudsen, Lotte Bjergbaek, Anni Hangaard Andersen   +6 more
wiley   +1 more source

Dimensional Coupling in Synergistic Bio‐Nano Growth Systems

MedComm – Biomaterials and Applications, Volume 4, Issue 2, June 2025.
Dimensional Evolution in Bio‐Nano Synergistic Systems: Proteins shrink into fibrils while gold ions expand into nanoparticles, minimizing bio‐nano interface interactions for enhanced hybrid functionality. This study highlights a competitive yet synergistic growth mechanism, offering insights for the rational design of bio‐nano materials with clinical ...
Ming Yuan, Xinmin Zhao, Jun Guo, Feng Zhang   +3 more
wiley   +1 more source

Characterization of nuclear structures containing superhelical DNA

Journal of Cell Science, 1976
ABSTRACT Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoiled and circular.
Cook, P, Brazell, I, Jost, E
openaire   +3 more sources