Animals, 2023 The genus Capripoxvirus belongs to the Poxviridae family. The sheeppox, goatpox, and lumpy skin disease viruses are three species of this genus with 96% identity in their genomes.
Ghulam Mustafa +6 more
doaj +3 more sources
The Pseudorabies Virus DNA Polymerase Accessory Subunit UL42 Directs Nuclear Transport of the Holoenzyme [PDF]
Frontiers in Microbiology, 2016 Pseudorabies virus (PRV) DNA replication occurs in the nuclei of infected cells and requires the viral DNA polymerase. The PRV DNA polymerase comprises a catalytic subunit, UL30, and an accessory subunit, UL42, that confers processivity to the enzyme ...
Yi-Ping eWang +6 more
doaj +3 more sources
DNA-directed termination of RNA polymerase II transcription [PDF]
Molecular Cell, 2023 RNA polymerase II (RNAPII) transcription involves initiation from a promoter, transcriptional elongation through the gene, and termination in the terminator region. In bacteria, terminators often contain specific DNA elements provoking polymerase dissociation, but RNAPII transcription termination is thought to be driven entirely by protein co-factors ...
Zhong Han +7 more
openaire +3 more sources
Human p53 interacts with the elongating RNAPII complex and is required for the release of actinomycin D induced transcription blockage [PDF]
, 2017 The p53 tumour suppressor regulates the transcription initiation of selected genes by binding to specific DNA sequences at their promoters. Here we report a novel role of p53 in transcription elongation in human cells.
Boros, I.M. (Imre M.) +7 more
core +12 more sources
Single-molecule view of coordination in a multi-functional DNA polymerase
eLife, 2021 Replication and repair of genomic DNA requires the actions of multiple enzymatic functions that must be coordinated in order to ensure efficient and accurate product formation.
Raymond F Pauszek III +3 more
doaj +1 more source
RNA-mediated in vivo Directed Evolution in Yeast
Bio-Protocol, 2022 Directed evolution is a powerful approach to obtain genetically-encoded sought-for traits. Compared to the prolonged adaptation regimes to mutations occurring under natural selection, directed evolution unlocks rapid screening and selection of mutants ...
Emil Jensen, Michael Jensen
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Polymerase Mu Is a DNA-Directed DNA/RNA Polymerase [PDF]
Molecular and Cellular Biology, 2003 DNA polymerases are defined as such because they use deoxynucleotides instead of ribonucleotides with high specificity. We show here that polymerase mu (pol μ), implicated in the nonhomologous end-joining pathway for repair of DNA double-strand breaks, incorporates both ribonucleotides and deoxynucleotides in a template-directed manner.
Stephanie A, Nick McElhinny +1 more
openaire +2 more sources
SHH1, a homeodomain protein required for DNA methylation, as well as RDR2, RDM4, and chromatin remodeling factors, associate with RNA polymerase IV. [PDF]
PLoS Genetics, 2011 DNA methylation is an evolutionarily conserved epigenetic modification that is critical for gene silencing and the maintenance of genome integrity. In Arabidopsis thaliana, the de novo DNA methyltransferase, domains rearranged methyltransferase 2 (DRM2),
Julie A Law +3 more
doaj +1 more source
ESTABLISHMENT OF A RAPID SCREENING METHOD FOR IN VITRO SITE-DIRECTED MUTANTS OR CRISPR/Cas9-MEDIA-TED KNOCK-IN MUTANTS [PDF]
精准医学杂志, 2023 Objective To establish a rapid screening method for in vitro site-directed mutants or CRISPR/Cas9-mediated knock-in mutants, and to improve mutant screening efficiency and reduce experimental costs.
SONG Chunlin, ZHANG Yuqing, ZHAI Yujing, ZHANG Gang, WANG Ying
doaj +1 more source
POLH (polymerase (DNA direct), eta) [PDF]
Atlas of Genetics and Cytogenetics in Oncology and Haematology, 2011 Review on POLH (polymerase (DNA direct), eta), with data on DNA, on the protein encoded, and where the gene is implicated.
A Stary, A Sarasin
openaire +1 more source