T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis [PDF]
Biotechnology & Biotechnological Equipment, 2016 Site-directed mutagenesis (SDM) is a useful tool to study the functions of regulatory sequences of DNA and RNA, and the structures and functions of proteins. Numerous methods have been developed for either single or multiple SDM (MSDM).
Wei Guo +4 more
doaj +2 more sources
Animals, 2023 The genus Capripoxvirus belongs to the Poxviridae family. The sheeppox, goatpox, and lumpy skin disease viruses are three species of this genus with 96% identity in their genomes.
Ghulam Mustafa +6 more
doaj +3 more sources
Human p53 interacts with the elongating RNAPII complex and is required for the release of actinomycin D induced transcription blockage [PDF]
, 2017 The p53 tumour suppressor regulates the transcription initiation of selected genes by binding to specific DNA sequences at their promoters. Here we report a novel role of p53 in transcription elongation in human cells.
Boros, I.M. (Imre M.) +7 more
core +12 more sources
Single-molecule view of coordination in a multi-functional DNA polymerase
eLife, 2021 Replication and repair of genomic DNA requires the actions of multiple enzymatic functions that must be coordinated in order to ensure efficient and accurate product formation.
Raymond F Pauszek III +3 more
doaj +1 more source
RNA-mediated in vivo Directed Evolution in Yeast
Bio-Protocol, 2022 Directed evolution is a powerful approach to obtain genetically-encoded sought-for traits. Compared to the prolonged adaptation regimes to mutations occurring under natural selection, directed evolution unlocks rapid screening and selection of mutants ...
Emil Jensen, Michael Jensen
doaj +1 more source
SHH1, a homeodomain protein required for DNA methylation, as well as RDR2, RDM4, and chromatin remodeling factors, associate with RNA polymerase IV. [PDF]
PLoS Genetics, 2011 DNA methylation is an evolutionarily conserved epigenetic modification that is critical for gene silencing and the maintenance of genome integrity. In Arabidopsis thaliana, the de novo DNA methyltransferase, domains rearranged methyltransferase 2 (DRM2),
Julie A Law +3 more
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ESTABLISHMENT OF A RAPID SCREENING METHOD FOR IN VITRO SITE-DIRECTED MUTANTS OR CRISPR/Cas9-MEDIA-TED KNOCK-IN MUTANTS [PDF]
精准医学杂志, 2023 Objective To establish a rapid screening method for in vitro site-directed mutants or CRISPR/Cas9-mediated knock-in mutants, and to improve mutant screening efficiency and reduce experimental costs.
SONG Chunlin, ZHANG Yuqing, ZHAI Yujing, ZHANG Gang, WANG Ying
doaj +1 more source
Dynamic regulation of ARGONAUTE4 within multiple nuclear bodies in Arabidopsis thaliana. [PDF]
PLoS Genetics, 2008 DNA methylation directed by 24-nucleotide small RNAs involves the small RNA-binding protein ARGONAUTE4 (AGO4), and it was previously shown that AGO4 localizes to nucleolus-adjacent Cajal bodies, sites of snRNP complex maturation. Here we demonstrate that
Carey F Li +5 more
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Kinetic analysis of an efficient DNA-dependent TNA polymerase. [PDF]
, 2005 alpha-l-Threofuranosyl nucleoside triphosphates (tNTPs) are tetrafuranose nucleoside derivatives and potential progenitors of present-day beta-d-2'-deoxyribofuranosyl nucleoside triphosphates (dNTPs).
Chaput, John C +6 more
core +2 more sources
Co-targeting RNA Polymerases IV and V Promotes Efficient De Novo DNA Methylation in Arabidopsis. [PDF]
, 2019 The RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine DNA methylation. The ability to manipulate RdDM would shed light on the mechanisms and applications of DNA methylation to control gene expression.
Chory, Joanne +9 more
core +2 more sources