Results 11 to 20 of about 25,728 (138)
Essential Oils Prime Epigenetic and Metabolomic Changes in Tomato Defense Against Fusarium oxysporum
Frontiers in Plant Science, 2022 In this work, we studied the direct and indirect plant protection effects of an Artemisia absinthium essential oil (AEO) on tomato seedlings against Fusarium oxysporum sp. oxysporum radicis lycopersici (Fol). AEO exhibited a toxic effect in vitro against
Serine Soudani +5 more
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A Bayesian approach to analyse genetic variation within RNA viral populations. [PDF]
PLoS Computational Biology, 2011 The development of modern and affordable sequencing technologies has allowed the study of viral populations to an unprecedented depth. This is of particular interest for the study of within-host RNA viral populations, where variation due to error-prone ...
Trevelyan J McKinley +4 more
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RNase-like domain in DNA-directed RNA polymerase II. [PDF]
Proceedings of the National Academy of Sciences, 1991 DNA-directed RNA polymerase is responsible for gene expression. Despite its importance, many details of its function and higher-order structure still remain unknown. We report here a local sequence similarity between the second largest subunit of RNA polymerase II and bacterial RNases Ba (barnase), Bi, and St. The most remarkable similarity is that the
T, Shirai, M, Go
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Functional Consequences of Subunit Diversity in RNA Polymerases II and V
Cell Reports, 2012 Multisubunit RNA polymerases IV and V (Pol IV and Pol V) evolved as specialized forms of Pol II that mediate RNA-directed DNA methylation (RdDM) and transcriptional silencing of transposons, viruses, and endogenous repeats in plants.
Ek Han Tan +3 more
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Use of Site-Specifically Tethered Chemical Nucleases to Study Macromolecular Reactions
Biological Procedures Online, 2003 During a complex macromolecular reaction multiple changes in molecular conformation and interactions with ligands may occur. X-ray crystallography may provide only a limited set of snapshots of these changes.
Mukherjee Srabani, Sousa Rui
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The RNA silencing enzyme RNA polymerase v is required for plant immunity. [PDF]
PLoS Genetics, 2011 RNA-directed DNA methylation (RdDM) is an epigenetic control mechanism driven by small interfering RNAs (siRNAs) that influence gene function. In plants, little is known of the involvement of the RdDM pathway in regulating traits related to immune ...
Ana López +4 more
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International Journal of Molecular Sciences, 2023 Emerging Mycobacterium tuberculosis (Mtb) resistant strains have continued to limit the efficacies of existing antitubercular therapies. More specifically, mutations in the RNA replicative machinery of Mtb, RNA polymerase (RNAP), have been widely linked to rifampicin (RIF) resistance, which has led to therapeutic failures in many clinical cases ...
Mokgerwa Zacharia Monama +2 more
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Primer Extension Reactions for the PCR- based α- complementation Assay
Bio-Protocol, 2015 The PCR- based- α- complementation assay is an effective technique to measure the fidelity of polymerases, especially RNA-dependent RNA polymerases (RDRP) and Reverse Transcriptases (RT). It has been successfully employed to determine the fidelity of the
Vasudevan Achuthan, Jeffrey DeStefano
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Cell Reports, 2014 Summary: Unlike nuclear multisubunit RNA polymerases I, II, and III, whose subunit compositions are conserved throughout eukaryotes, plant RNA polymerases IV and V are nonessential, Pol II-related enzymes whose subunit compositions are still evolving ...
Jeremy R. Haag +13 more
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Isolation and physical properties of the DNA‐directed RNA polymerase from Thermus thermophilus HB8 [PDF]
European Journal of Biochemistry, 1990 The DNA‐directed RNA polymerase from the extremely thermophilic eubacterium Thermus thermophilus HB8 was purified employing a new and rapid method. The subunit pattern of the enzyme, analyzed by SDS gel electrophoresis, was interpreted as: 140 kDa and 170 kDa for β and β′, 40 kDa for α and 92 kDa for ó.
S, Wnendt +3 more
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