Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates [PDF]
, 2013 Nonhomologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although it is not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA
Bartlett, Edward +2 more
core +1 more source
Fluorescent labeling of plasmid DNA and mRNA : gains and losses of current labeling strategies [PDF]
, 2016 Live-cell imaging has provided the life sciences with insights into the cell biology and dynamics. Fluorescent labeling of target molecules proves to be indispensable in this regard. In this Review, we focus on the current fluorescent labeling strategies
Rombouts, Koen +2 more
core +1 more source
RNA polymerase II stalling promotes nucleosome occlusion and pTEFb recruitment to drive immortalization by Epstein-Barr virus [PDF]
, 2011 Epstein-Barr virus (EBV) immortalizes resting B-cells and is a key etiologic agent in the development of numerous cancers. The essential EBV-encoded protein EBNA 2 activates the viral C promoter (Cp) producing a message of ~120 kb that is differentially ...
A Bakos +75 more
core +3 more sources
Entropy involved in fidelity of DNA replication [PDF]
, 2012 Information has an entropic character which can be analyzed within the Statistical Theory in molecular systems. R. Landauer and C.H. Bennett showed that a logical copy can be carried out in the limit of no dissipation if the computation is performed ...
Gonzalo G. de Polavieja +1 more
core +8 more sources
A sigma factor toolbox for orthogonal gene expression in Escherichia coli [PDF]
, 2018 Synthetic genetic sensors and circuits enable programmable control over timing and conditions of gene expression and, as a result, are increasingly incorporated into the control of complex and multi-gene pathways.
Bervoets, Indra +6 more
core +4 more sources
Spt5 Cooperates with Human Immunodeficiency Virus Type 1 Tat by Preventing Premature RNA Release at Terminator Sequences [PDF]
, 2002 The human immunodeficiency virus type 1 (HIV-1) Tat protein activates transcription elongation by stimulating the Tat-activated kinase (TAK/p-TEFb), a protein kinase composed of CDK9 and its cyclin partner, cyclin T1.
Bourgeois, Cyril F +4 more
core +3 more sources
Template-directed biopolymerization: tape-copying Turing machines
, 2012 DNA, RNA and proteins are among the most important macromolecules in a living cell. These molecules are polymerized by molecular machines. These natural nano-machines polymerize such macromolecules, adding one monomer at a time, using another linear ...
Chowdhury, Debashish, Sharma, Ajeet K.
core +1 more source
TFE and Spt4/5 open and close the RNA polymerase clamp during the transcription cycle [PDF]
, 2016 Transcription is an intrinsically dynamic process and requires the coordinated interplay of RNA polymerases (RNAPs) with nucleic acids and transcription factors.
Gietl, Andreas +5 more
core +1 more source
Characterization of the Products of RNA-directed DNA Polymerases in Oncogenic RNA Viruses
Nature, 1970 Several RNA tumour viruses contain an enzyme that synthesizes a DNA–RNA hybrid using the single stranded viral RNA as template. Hybridization experiments confirm that the DNA strand is complementary to the viral RNA.
Spiegelman, S. +6 more
openaire +4 more sources
Mode of inhibition of HIV reverse transcriptase by 2-hexaprenylhydroquinone, a novel general inhibitor of RNA-and DNA-directed DNA polymerases [PDF]
Biochemical Journal, 1997 A natural compound from the Red Sea sponge Ircinia sp., 2-hexaprenylhydroquinone (HPH), has been shown to be a general inhibitor of retroviral reverse transcriptases (from HIV-1, HIV-2 and murine leukaemia virus) as well as of cellular DNA polymerases (Escherichia coli DNA polymerase I, and DNA polymerases α and β).
Amnon Hizi +3 more
openaire +3 more sources