Results 1 to 10 of about 54,243 (194)

Stationary-phase Pseudomonas aeruginosa fluoroquinolone persisters mostly avoid DNA double-stranded breaks [PDF]

mSphere
When susceptible bacterial cultures are treated with antibiotics, some cells can survive treatment without heritable resistance, giving rise to susceptible daughter cells in a phenomenon termed antibiotic persistence.
Patricia J. Hare, Juliet R. González, Wendy W. K. Mok   +2 more
doaj   +2 more sources

Theoretical, biological, and electrochemical explanation of the complexity of Peramivir’s drug–DNA interaction [PDF]

Scientific Reports
In this study, an in-depth investigation was carried out on the interaction between the anti-influenza drug peramivir (PMV) and various DNA, including double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), polyadenylic acid (PolyA), polyguanylic acid (
Monika Wypych, Anna Ignaczak, Paulina Tokarz, Jagoda Seroka, Kamila Koszelska, Sylwia Smarzewska   +5 more
doaj   +2 more sources

UPF1 promotes the formation of R loops to stimulate DNA double-strand break repair

Nature Communications, 2021
R loops are formed when single-stranded RNA anneals to one strand of DNA, forming three-stranded structures containing DNA-RNA hybrids and the displaced non-template single-stranded DNA. Here the authors reveal that the DNA:RNA helicase UPF1 plays a role
Greg H. P. Ngo, Julia W. Grimstead, Duncan M. Baird   +2 more
doaj   +1 more source

Immediate-Early, Early, and Late Responses to DNA Double Stranded Breaks

Frontiers in Genetics, 2022
Loss or rearrangement of genetic information can result from incorrect responses to DNA double strand breaks (DSBs). The cellular responses to DSBs encompass a range of highly coordinated events designed to detect and respond appropriately to the damage,
Shaylee R. Kieffer, Noel F. Lowndes
doaj   +1 more source

Function and Regulation of Nuclear DNA Sensors During Viral Infection and Tumorigenesis

Frontiers in Immunology, 2021
IFI16, hnRNPA2B1, and nuclear cGAS are nuclear-located DNA sensors that play important roles in initiating host antiviral immunity and modulating tumorigenesis.
Fan Zhang, Fan Zhang, Yi Yuan, Yi Yuan, Yi Yuan, Feng Ma, Feng Ma   +6 more
doaj   +1 more source

Splicing stimulates siRNA formation at Drosophila DNA double-strand breaks. [PDF]

PLoS Genetics, 2017
DNA double-strand breaks trigger the production of locus-derived siRNAs in fruit flies, human cells and plants. At least in flies, their biogenesis depends on active transcription running towards the break.
Karin Merk, Marco Breinig, Romy Böttcher, Stefan Krebs, Helmut Blum, Michael Boutros, Klaus Förstemann   +6 more
doaj   +1 more source

Protocol to measure end resection intermediates at sequence-specific DNA double-strand breaks by quantitative polymerase chain reaction using ER-AsiSI U2OS cells

STAR Protocols, 2022
Summary: DNA end resection is a critical step in the homologous recombination pathway of repairing DNA double-strand breaks (DSBs) that can be visualized in cells by detecting the generation of single-stranded DNA (ssDNA) intermediates formed during the ...
Ajit K. Sharma, Amira Mohammed Fitieh, Jana Yasser Hafez Ali, Ismail Hassan Ismail   +3 more
doaj   +1 more source

Human APOBEC3A isoforms translocate to the nucleus and induce DNA double strand breaks leading to cell stress and death. [PDF]

PLoS ONE, 2013
Human APOBEC3 enzymes deaminate single stranded DNA. At least five can deaminate mitochondrial DNA in the cytoplasm, while three can deaminate viral DNA in the nucleus. However, only one, APOBEC3A, can hypermutate genomic DNA.
Bianka Mussil, Rodolphe Suspène, Marie-Ming Aynaud, Anne Gauvrit, Jean-Pierre Vartanian, Simon Wain-Hobson   +5 more
doaj   +1 more source

Sperm DNA integrity does play a crucial role for embryo development after ICSI, notably when good-quality oocytes from young donors are used

Biological Research, 2022
Based on the inconsistent literature published thus far involving infertile patients, whether intracytoplasmic sperm injection (ICSI) allows overcoming total fertilization failure due to sperm DNA fragmentation is still unclear.
Jordi Ribas-Maynou, Sergi Novo, Marc Torres, Albert Salas-Huetos, Sergi Rovira, Marta Antich, Marc Yeste   +6 more
doaj   +1 more source

COMMD4 functions with the histone H2A-H2B dimer for the timely repair of DNA double-strand breaks

Communications Biology, 2021
Amila Suraweera et al. use a range of biochemical and in vitro cellular assays to examine the role of the COMMD4 in DNA repair. Their results suggest that COMMD4 interacts with the histone H2A-H2B during repair of double-stranded DNA breaks, thereby ...
Amila Suraweera, Neha S. Gandhi, Sam Beard, Joshua T. Burgess, Laura V. Croft, Emma Bolderson, Ali Naqi, Nicholas W. Ashton, Mark N. Adams, Kienan I. Savage, Shu-Dong Zhang, Kenneth J. O’Byrne, Derek J. Richard   +12 more
doaj   +1 more source