DNA resection in eukaryotes: deciding how to fix the break [PDF]
, 2010 DNA double-strand breaks are repaired by different mechanisms, including homologous recombination and nonhomologous end-joining. DNA-end resection, the first step in recombination, is a key step that contributes to the choice of DSB repair.
A Aguilera +74 more
core +1 more source
Suboptimal extracellular pH values alter DNA damage response to induced double‐strand breaks
FEBS Open Bio, 2018 Conditions leading to unrepaired DNA double‐stranded breaks are potent inducers of genetic instability. Systemic conditions may lead to fluctuation of hydrogen ions in the cellular microenvironment, and we show that small variations in extracellular pH ...
Julien Massonneau +5 more
doaj +1 more source
The human DNA glycosylases NEIL1 and NEIL3 excise psoralen-induced DNA-DNA cross-links in a four-stranded DNA structure [PDF]
, 2017 Interstrand cross-links (ICLs) are highly cytotoxic DNA lesions that block DNA replication and transcription by preventing strand separation. Previously, we demonstrated that the bacterial and human DNA glycosylases Nei and NEIL1 excise unhooked ...
A Katafuchi +44 more
core +4 more sources
Chromatin Remodeling at DNA Double-Strand Breaks [PDF]
Cell, 2013 DNA double-strand breaks (DSBs) can arise from multiple sources, including exposure to ionizing radiation. The repair of DSBs involves both posttranslational modification of nucleosomes and concentration of DNA-repair proteins at the site of damage. Consequently, nucleosome packing and chromatin architecture surrounding the DSB may limit the ability of
Price, Brendan D., D’Andrea, Alan D.
openaire +2 more sources
Stitching up broken DNA ends by FANCA
Molecular & Cellular Oncology, 2018 RAD52 rejoins resected broken DNA ends by mediating single-strand annealing. Our recent work elucidates that FANCA, a Fanconi anemia protein, also directly repairs double-strand breaks (DSBs) by catalyzing annealing of single-stranded DNA.
Anna Palovcak +3 more
doaj +1 more source
TUNEL Labeling to Detect Double-stranded DNA Breaks in Caenorhabditis elegans Gonads
Bio-Protocol, 2022 Analysis of DNA double strand breaks (DSBs) is important for understanding dyshomeostasis within the nucleus, impaired DNA repair mechanisms, and cell death. In the C.
Peter Kropp +2 more
doaj +1 more source
Identification of a novel motif in DNA ligases exemplified by DNA ligase IV [PDF]
, 2006 DNA ligase IV is an essential protein that functions in DNA non-homologous end-joining, the major mechanism that rejoins DNA double-strand breaks in mammalian cells. LIG4 syndrome represents a human disorder caused by mutations in DNA ligase IV that lead
Doherty, Aidan J +5 more
core +1 more source
Oxidative stress-induced DNA damage and repair in human peripheral blood mononuclear cells: protective role of hemoglobin. [PDF]
PLoS ONE, 2013 BACKGROUND:DNA repair is a cellular defence mechanism responding to DNA damage caused in large part by oxidative stress. There is a controversy with regard to the effect of red blood cells on DNA damage and cellular response.
Anat Gafter-Gvili +8 more
doaj +1 more source
A type I IFN-dependent DNA damage response regulates the genetic program and inflammasome activation in macrophages [PDF]
, 2017 Macrophages produce genotoxic agents, such as reactive oxygen and nitrogen species, that kill invading pathogens. Here we show that these agents activate the DNA damage response (DDR) kinases ATM and DNA-PKcs through the generation of double stranded ...
Akira +67 more
core +3 more sources
TCDD Affects DNA Double Strand-Break Repair [PDF]
Toxicological Sciences, 2004 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), an environmental toxicant, elicits a spectrum of deleterious biological responses including carcinogenesis. We hypothesize that TCDD exposure exerts its carcinogenicity, in part, by affecting the repair of DNA double strand breaks (DSBs) through homologous recombination (HR), mediated by the AhR signaling ...
Clara Y Y, Chan +2 more
openaire +2 more sources