Results 21 to 30 of about 104,786 (306)

Human APOBEC3A isoforms translocate to the nucleus and induce DNA double strand breaks leading to cell stress and death. [PDF]

PLoS ONE, 2013
Human APOBEC3 enzymes deaminate single stranded DNA. At least five can deaminate mitochondrial DNA in the cytoplasm, while three can deaminate viral DNA in the nucleus. However, only one, APOBEC3A, can hypermutate genomic DNA.
Bianka Mussil, Rodolphe Suspène, Marie-Ming Aynaud, Anne Gauvrit, Jean-Pierre Vartanian, Simon Wain-Hobson   +5 more
doaj   +1 more source

Protocol to measure end resection intermediates at sequence-specific DNA double-strand breaks by quantitative polymerase chain reaction using ER-AsiSI U2OS cells

STAR Protocols, 2022
Summary: DNA end resection is a critical step in the homologous recombination pathway of repairing DNA double-strand breaks (DSBs) that can be visualized in cells by detecting the generation of single-stranded DNA (ssDNA) intermediates formed during the ...
Ajit K. Sharma, Amira Mohammed Fitieh, Jana Yasser Hafez Ali, Ismail Hassan Ismail   +3 more
doaj   +1 more source

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair. [PDF]

, 2020
Repair of double strand DNA breaks (DSBs) can result in gene disruption or gene modification via homology directed repair (HDR) from donor DNA. Altering cellular responses to DSBs may rebalance editing outcomes towards HDR and away from other repair ...
A Ferreira, A Montagnoli, A Orthwein, AC Komor, B Roberts, B Verdoodt, C Anders, C Yu, CD Richardson, CD Richardson, D Szklarczyk, DW Huang, E Lingeman, ES Koltun, FK Zimmermann, H Ghezraoui, J Song, JA Doudna, JE Haber, JP Duxin, K Jayathilaka, K Nakanishi, K Okita, KJ Livak, L Lei, L Pessoa-Brandão, LA Gilbert, LN Brandão, M Cavazzana, M Lei, M Yamada, MA DeWitt, MD Canny, MD Rainey, MH Porteus, N Hustedt, N Zielke, NK Sasi, NR Pannunzio, R Ceccaldi, RC Alver, S Hughes, S Lin, SB Thyme, T Maruyama, T Takenaka, T Tsuji, TA Day, TL Roth, TS Nambiar, U Eichenlaub-Ritter, VT Chu, W Li, X Liang, Z Romero   +54 more
core   +1 more source

Sperm DNA integrity does play a crucial role for embryo development after ICSI, notably when good-quality oocytes from young donors are used

Biological Research, 2022
Based on the inconsistent literature published thus far involving infertile patients, whether intracytoplasmic sperm injection (ICSI) allows overcoming total fertilization failure due to sperm DNA fragmentation is still unclear.
Jordi Ribas-Maynou, Sergi Novo, Marc Torres, Albert Salas-Huetos, Sergi Rovira, Marta Antich, Marc Yeste   +6 more
doaj   +1 more source

Srs2 Disassembles Rad51 Filaments by a Protein-Protein Interaction Triggering ATP Turnover and Dissociation of Rad51 from DNA [PDF]

, 2009
Rad51 is a DNA recombinase functioning in the repair of DNA double-strand breaks and the generation of genetic diversity by homologous recombination (HR).
Antony, Edwin, Ellenberger, Tom, Krejci, Lumir, Lohman, Timothy M., Tomko, Eric J., Xiao, Qi   +5 more
core   +2 more sources

COMMD4 functions with the histone H2A-H2B dimer for the timely repair of DNA double-strand breaks

Communications Biology, 2021
Amila Suraweera et al. use a range of biochemical and in vitro cellular assays to examine the role of the COMMD4 in DNA repair. Their results suggest that COMMD4 interacts with the histone H2A-H2B during repair of double-stranded DNA breaks, thereby ...
Amila Suraweera, Neha S. Gandhi, Sam Beard, Joshua T. Burgess, Laura V. Croft, Emma Bolderson, Ali Naqi, Nicholas W. Ashton, Mark N. Adams, Kienan I. Savage, Shu-Dong Zhang, Kenneth J. O’Byrne, Derek J. Richard   +12 more
doaj   +1 more source

Homologous Recombination under the Single-Molecule Fluorescence Microscope [PDF]

, 2019
Homologous recombination (HR) is a complex biological process and is central to meiosis and for repair of DNA double-strand breaks. Although the HR process has been the subject of intensive study for more than three decades, the complex protein–protein ...
Dhakal, Soma, Gibbs, Dalton R.
core   +2 more sources

High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast. [PDF]

, 2017
In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule.
Dominska, Margaret, Petes, Thomas D, Yim, Eunice, Yin, Yi   +3 more
core   +3 more sources

The SAGA Deubiquitination Module Promotes DNA Repair and Class Switch Recombination through ATM and DNAPK-Mediated γH2AX Formation

Cell Reports, 2016
Summary: Class switch recombination (CSR) requires activation-induced deaminase (AID) to instigate double-stranded DNA breaks at the immunoglobulin locus.
Shaliny Ramachandran, Dania Haddad, Conglei Li, Michael X. Le, Alexanda K. Ling, Clare C. So, Rajeev M. Nepal, Jennifer L. Gommerman, Kefei Yu, Troy Ketela, Jason Moffat, Alberto Martin   +11 more
doaj   +1 more source

Increased Chlormethine-Induced DNA Double-Stranded Breaks in Malignant T Cells from Mycosis Fungoides Skin Lesions

JID Innovations, 2022
Mycosis fungoides (MF) is a type of cutaneous T-cell lymphoma. Chlormethine (CL) is recommended as first-line therapy for MF, with a major purpose to kill tumor cells through DNA alkylation.
Yun-Tsan Chang, Desislava Ignatova, Wolfram Hoetzenecker, Steve Pascolo, Christina Fassnacht, Emmanuella Guenova   +5 more
doaj   +1 more source