Results 21 to 30 of about 54,243 (194)
PAXX promotes KU accumulation at DNA breaks and is essential for end-joining in XLF-deficient mice
Nature Communications, 2017 Non-homologous end-joining is the key pathway for repairing double-stranded DNA breaks in mammalian cells. Here the authors show that PAXX promotes the accumulation of KU at DNA breaks and is essential for end-joining in cells lacking XLF.
Xiangyu Liu +4 more
doaj +1 more source
Sliding to the rescue of damaged DNA
eLife, 2012 Single-molecule imaging experiments have shed new light on the methods used by the enzyme RecA to align single- and double-stranded DNA so that double-strand breaks can be repaired.
Bryan Gibb, Eric C Greene
doaj +1 more source
Homologous pairing activities of two rice RAD51 proteins, RAD51A1 and RAD51A2. [PDF]
PLoS ONE, 2013 In higher eukaryotes, RAD51 functions as an essential protein in homologous recombination and recombinational repair of DNA double strand breaks. During these processes, RAD51 catalyzes homologous pairing between single-stranded DNA and double-stranded ...
Yuichi Morozumi +8 more
doaj +1 more source
G-quadruplex Structures Contribute to Differential Radiosensitivity of the Human Genome
iScience, 2019 Summary: DNA, the fundamental unit of human cell, generally exists in Watson-Crick base-paired B-DNA form. Often, DNA folds into non-B forms, such as four-stranded G-quadruplexes.
Nitu Kumari +7 more
doaj +1 more source
CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway
Protein & Cell, 2020 With its high efficiency for site-specific genome editing and easy manipulation, the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR associated protein 9 (CAS9) system has become the most widely used gene editing technology in ...
Shuxiang Xu +6 more
doaj +1 more source
Local unwinding of double-strand DNA ends by the MRX complex promotes Exo1 processing activity
Molecular & Cellular Oncology, 2018 Homologous recombination is initiated by nucleolytic degradation (resection) of DNA double-strand breaks (DSBs), which involves different nucleases including the Mre11-Rad50-Xrs2 (MRX) complex and the Exonuclease 1 (Exo1). The characterization of a novel
Elisa Gobbini +2 more
doaj +1 more source
Filling in the gaps in PARP inhibitor-induced synthetic lethality
Molecular & Cellular Oncology, 2021 Tumors with loss of breast cancer type 1 susceptibility protein (BRCA1) are homologous recombination (HR) deficient and hypersensitive to poly(ADP-ribose) polymerase inhibitors (PARPi).
Mariana Paes Dias, Jos Jonkers
doaj +1 more source
The newfound relationship between extrachromosomal DNAs and excised signal circles
FEBS Letters, EarlyView.Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley +1 more source
The origin of unwanted editing byproducts in gene editing
Acta Biochimica et Biophysica Sinica, 2022 The rapid development of CRISPR-Cas genome editing tools has greatly changed the way to conduct research and holds tremendous promise for clinical applications.
Yin Jianhang, Hu Jiazhi
doaj +1 more source
FEBS Letters, EarlyView.Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson +7 more
wiley +1 more source