Results 41 to 50 of about 11,641,417 (251)

Copy number analyses of DNA repair genes reveal the role of poly(ADP-ribose) polymerase (PARP) in tree longevity

iScience, 2021
Summary: Long-lived organisms are exposed to the risk of accumulating mutations due to DNA damage. Previous studies in animals have revealed the positive relationship between the copy number of DNA repair genes and longevity.
Yuta Aoyagi Blue, Junko Kusumi, Akiko Satake   +2 more
doaj   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

The role of genomic Copy Number Variation (CNV) in osteoporosis [PDF]

, 2012
Copy number variation (CNV) is a relatively novel source of genetic variation, involving the duplication or deletion of segments of genomic DNA (gDNA) sequence, thereby changing the original number of DNA copies.
Connolly, Kate
core  

Suitability of a CMV/EGFP cassette to monitor stable expression from human artificial chromosomes but not transient transfer in the cells forming viable clones [PDF]

, 2004
Human artificial chromosomes (HACs) were generated by transfer of telomerized PAC constructs containing alpha satellite DNA of various human chromosomes. To monitor which cells took up constructs and subsequently formed stable clones under blasticidin S (
Laner, A., Schindelhauer, D., Christan, S., Schwarz, T., Laner, A.;Schwarz, T.;Christan, S.;Schindelhauer, D.   +4 more
core   +1 more source

DNA copy number variations (14 cancer-associated genes) in non-small cell lung cancer

Якутский медицинский журнал
Non-small cell lung cancer (NSCLC) accounts for 85% of all lung cancers, 15-30% of which is squamous cell carcinoma, 54% is adenocarcinoma. The copy number variations (CNVs) as one of the factors affecting gene transcription activity is necessary to ...
R. D. Al-Nsour, N. A. Petrusenko, V. V. Olekseenko, A. Y. Maksimov, O. N. Stateshn   +4 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Copy number, linkage disequilibrium and disease association in the FCGR locus [PDF]

, 2010
The response of a leukocyte to immune complexes (ICs) is modulated by receptors for the Fc region of IgG (FcgammaRs), and alterations in their affinity or function have been associated with risk of autoimmune diseases, including systemic lupus ...
Padyukov, L, T. F. Rayner, Willcocks, Lisa C, W. Yang, L. C. Willcocks, J. A. G. Scott, S. J. Dunstan, Rayner, Tim F, I. Gunnarsson, Niederer, Heather A., Smith, KG, Peshu, N, B. C. Urban, Svenungsson, Elisabet, Scott, John, Clayton, David G., P. A. Lyons, Niederer, Heather A, Williams, TN, Lyons, Paul A., Urban, Britta, Scott, J. Anthony G., Y. L. Lau, Yang, Wanling, Williams, Thomas N., Willcocks, LC, L. Padyukov, Clayton, David G, N. Peshu, Watts, Richard A., Savage, Caroline, Yang, W, Dunstan, SJ, Dunstan, Sarah, Urban, BC, Watts, RA, N. H. Phu, T. T. Hien, Phu, Nguyen Hoan, Rayner, TF, Scott, JAG, Hien, Tran, Willcocks, Lisa C., Clayton, DG, Savage, CO, Lyons, PA, T. N. Williams, E. Svenungsson, K. G. C. Smith, Rayner, Tim F., Hien, TT, Hien, Tran Tinh, Svenungsson, E, Padyukov, Leonid, Smith, Kenneth G.C., Gunnarsson, I, Savage, Caroline O, Smith, Kenneth G C, Gunnarsson, Iva, Peshu, Norbert, Lau, YL, Phu, NH, Smith, KGC, Lau, Yu Lung, R. A. Watts, Watts, Richard A, Savage, Caroline O., Scott, JA, H. A. Niederer, Scott, J Anthony G, Urban, Britta C, Smith, Kenneth GC, Williams, Thomas N, C. O. Savage, D. G. Clayton, Niederer, HA, Williams, Thomas, Dunstan, Sarah J., Dunstan, Sarah J, Lyons, Paul A   +79 more
core   +2 more sources

An Integrated Approach for RNA-seq Data Normalization

Cancer Informatics, 2016
Background DNA copy number alteration is common in many cancers. Studies have shown that insertion or deletion of DNA sequences can directly alter gene expression, and significant correlation exists between DNA copy number and gene expression.
Shengping Yang, Donald E. Mercante, Kun Zhang, Zhide Fang   +3 more
doaj   +1 more source

A novel technique for measuring variations in DNA copy-number: competitive genomic polymerase chain reaction

BMC Genomics, 2007
Background Changes in genomic copy number occur in many human diseases including cancer. Characterization of these changes is important for both basic understanding and diagnosis of these diseases. Microarrays have recently become the standard technique
Nakagawara Akira, Kawamoto Shoko, Saito Sakae, Nakamura Yohko, Maekawa Kazunori, Iwao-Koizumi Kyoko, Kato Kikuya   +6 more
doaj   +1 more source