Results 141 to 150 of about 94,067 (257)

Molecular basis for methylation-sensitive editing by Cas9. [PDF]

Nature
Roth MO, Shu Y, Zhao Y, Trasanidou D, Hoffman RD, Südfeld C, Bouzetos E, Trasanidis N, Zawrotny M, Gelasco MK, Medina ML, Das A, Rai J, Goswami HN, Wang B, van der Oost J, Li H.   +16 more
europepmc   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, EarlyView.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

Draft genome sequence of epiphyte <i>Paenibacillus kyungheensis</i> strain Cap286 isolated from <i>Chenopodium album</i> L. leaves. [PDF]

Microbiol Resour Announc
Chebotar VK, Losev MR, Gancheva MS, Kudryavtcev DV, Bortsova OA, Chizhevskaya EP, Pishchik VN.   +6 more
europepmc   +1 more source

Optimizations of Cas12a‐ and Cas12i‐based adenine base editors for efficient precision editing in the plant genome

Journal of Integrative Plant Biology, EarlyView.
A strategy coupling high‐activity nucleases with dimeric TadA‐8e optimizes plant Cas12‐based adenine base editors, boosts editing efficiency, and provides precise editors for crop breeding and genomics research.
Xiaoshuang Liu, Shan Jin, Zhi Xiao, Chong Ma, Qing Wang, Huanhuan Wang, Ruohan Zhou, Dongfang Gu, Rongfang Xu, Ruiying Qin, Juan Li, Pengcheng Wei   +11 more
wiley   +1 more source

Efficient and Safe Knockout of <i>AR</i> and <i>DMRT1</i> Mediated by Cytosine Base Editors in Chicken DF-1 and PGCs. [PDF]

Vet Sci
Gao X, Tang N, Zhao Z, He Y, Shen Y, Zou X, Luo C.   +6 more
europepmc   +1 more source

Pentatricopeptide repeat proteins in crops: Advances in functional mechanisms and breeding applications

Journal of Integrative Plant Biology, EarlyView.
Pentatricopeptide repeat proteins coordinate nucleus‐organelle communication by modulating RNA metabolism within chloroplasts and mitochondria. This review highlights how they control critical processes like photosynthesis, seed development, fertility restoration, and stress survival in crops, and explores their potential as programmable tools for RNA ...
Mingming Wu, Maohong Cai, Rongrong Zhai, Jing Ye, Guofu Zhu, Faming Yu, Shenghai Ye, Xiaoming Zhang   +7 more
wiley   +1 more source

A Cytosine Deaminase-Based Genomic Footprinting Assay (cFOOT-seq) for Detecting Transcription Factor Occupancy. [PDF]

Bio Protoc
Yang MC, Wu A, Wang H, Liu X, Shi J, Gao S, Zhang JM.   +6 more
europepmc   +1 more source

Enhancing CRISPR‐Cas12a base editing in plants with LbCas12a variants and introns

Journal of Integrative Plant Biology, EarlyView.
Intron optimization of LbCas12a‐RRV improves cytosine and adenine base editing efficiency in plants, supports multiplexed and double‐strand break‐free genome modification, and expands precise genome engineering tools for crop breeding and plant functional research. ABSTRACT Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools
Yanhao Cheng, Gen Li, Man Zhou, Rushil Mandlik, Doris Wang, Yiping Qi   +5 more
wiley   +1 more source

Vipr2 Gene Expression Is Upregulated in the Nucleus Accumbens of Spontaneous Hypertensive Rats During Early Life. [PDF]

Neuropsychopharmacol Rep
Araki R, Kita A, Ago Y, Yabe T.
europepmc   +1 more source

Optimized TadA‐derived base editors efficiently manipulate mRNA splicing by A‐to‐G and C‐to‐K editing in potato

Journal of Integrative Plant Biology, EarlyView.
Two optimized TadA‐derived base editors efficiently generate diverse splicing variants by targeting specific splice sites in potato. ABSTRACT Pre‐messenger RNA (pre‐mRNA) splicing is a critical mechanism for post‐transcriptional regulation in plants. Through alternative splicing, plants produce diverse transcriptomes and proteomes that finely regulate ...
Kaiyuan Chen, Yan Zhang, Jiuzhou Deng, Lumin Zhang, Huiying Zhou, Yaxin Duan, Enle Xiao, Guangtao Zhu, Chunzhi Zhang   +8 more
wiley   +1 more source