Isolation of human and murine hematopoietic stem cells for DNA damage and DNA repair assays
STAR Protocols, 2021 Summary: Hematopoietic stem and progenitor cells (HSPCs) reside in the bone marrow and supply blood cells. Efficient methods for isolation of HSPCs are required. Here, we present protocols for the isolation of human and murine HSPCs using manual and FACS-
Alfredo Rodríguez +5 more
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Uncovering an allosteric mode of action for a selective inhibitor of human Bloom syndrome protein
eLife, 2021 BLM (Bloom syndrome protein) is a RECQ-family helicase involved in the dissolution of complex DNA structures and repair intermediates. Synthetic lethality analysis implicates BLM as a promising target in a range of cancers with defects in the DNA damage ...
Xiangrong Chen +13 more
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MRN complex is an essential effector of DNA damage repair
Journal of Zhejiang University SCIENCE B, 2021 Genome stability can be threatened by both endogenous and exogenous agents. Organisms have evolved numerous mechanisms to repair DNA damage, including homologous recombination (HR) and non-homologous end joining (NHEJ).
Shan Qiu, Jun Huang
semanticscholar +1 more source
Radiobiology Summaries: DNA Damage and Repair [PDF]
Clinical Oncology, 2021 No abstract available.
Chalmers, A.J., Carruthers, R.D.
openaire +3 more sources
PTEN in DNA damage repair [PDF]
Cancer Letters, 2012 The ability of DNA repair in a cell is vital to its genomic integrity and thus to the normal functioning of an organism. Phosphatase and tensin homolog (PTEN) is a well-established tumor suppressor gene that induces apoptosis and controls cell growth by inhibiting the PI3K/AKT pathway.
Mei, Ming, Yu-Ying, He
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Research progress on the role and mechanism of DNA damage repair in germ cell development
Frontiers in Endocrinology, 2023 In the complex and dynamic processes of replication, transcription, and translation of DNA molecules, a large number of replication errors or damage can occur which lead to obstacles in the development process of germ cells and result in a decreased ...
Yan Wang +9 more
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DNA Damage Repair and Current Therapeutic Approaches in Gastric Cancer: A Comprehensive Review
Frontiers in Genetics, 2022 DNA in cells is frequently damaged by endogenous and exogenous agents. However, comprehensive mechanisms to combat and repair DNA damage have evolved to ensure genomic stability and integrity.
Menghui Wang, Chuan Xie
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Cohesin causes replicative DNA damage by trapping DNA topological stress [PDF]
, 2020 DNA topological stress inhibits DNA replication fork (RF) progression and contributes to DNA replication stress. In Saccharomyces cerevisiae, we demonstrate that centromeric DNA and the rDNA array are especially vulnerable to DNA topological stress ...
Baxter, Jonathan +2 more
core +1 more source
Cooperation of the ATM and Fanconi Anemia/BRCA Pathways in Double-Strand Break End Resection
Cell Reports, 2020 Summary: Cells deficient in ataxia telangiectasia mutated (ATM) are hypersensitive to ionizing radiation and other anti-cancer agents that induce double-strand DNA breaks. ATM inhibitors may therefore sensitize cancer cells to these agents.
Mu-Yan Cai +9 more
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Suppression of isoprenylcysteine carboxylmethyltransferase compromises DNA damage repair
Life Science Alliance, 2021 Inhibition of isoprenylcysteine carboxylmethyltransferase reduces cancer cells’ ability to repair DNA damage by suppressing the expression of critical DNA damage repair pathway genes, hence increasing their vulnerability to DNA damaging insults such as ...
Jingyi Tang, Patrick J Casey, Mei Wang
doaj +1 more source