Results 151 to 160 of about 2,368,060 (250)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Satellite DNA Editing Enables Multiplexed Chromosome Restructuring in Populus. [PDF]

Plant Biotechnol J
Zhou R, Chen MSS, Drowns MR, Mailloux K, Yin K, Vaillancourt B, Buell CR, Tsai CJ.   +7 more
europepmc   +1 more source

DNA-editing enzymes as potential treatments for heteroplasmic mtDNA diseases. [PDF]

J Intern Med, 2020
Zekonyte U, Bacman SR, Moraes CT.
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Chemiluminescent Deoxyribozyme Sensors for DNA-Editing Enzymes. [PDF]

ACS Chem Biol
Jakubec M, Svoboda M, Kurfürst J, Svehlova K, Veverka V, Curtis EA.   +5 more
europepmc   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Dual-mode application of transcriptional regulation and DNA editing using the ultra-compact TnpB system. [PDF]

Nucleic Acids Res
Oh Y, Jeon SB, Gwon LW, Sim BW, Lee SH.
europepmc   +1 more source

Programming PAM antennae for efficient CRISPR-Cas9 DNA editing. [PDF]

Sci Adv, 2020
Wang F, Hao Y, Li Q, Li J, Zhang H, Zhang X, Wang L, Bustamante C, Fan C.   +8 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source