Results 31 to 40 of about 888,680 (335)
Editing DNA Methylation in Mammalian Embryos [PDF]
International Journal of Molecular Sciences, 2020 DNA methylation in mammals is essential for numerous biological functions, such as ensuring chromosomal stability, genomic imprinting, and X-chromosome inactivation through transcriptional regulation. Gene knockout of DNA methyltransferases and demethylation enzymes has made significant contributions to analyzing the functions of DNA methylation in ...
Taiga Yamazaki +4 more
openaire +2 more sources
Enhancement of homology-directed repair with chromatin donor templates in cells. [PDF]
, 2020 A key challenge in precise genome editing is the low efficiency of homology-directed repair (HDR). Here we describe a strategy for increasing the efficiency of HDR in cells by using a chromatin donor template instead of a naked DNA donor template.
Cruz-Becerra, Grisel, Kadonaga, James T
core +1 more source
No evidence of genome editing activity from Natronobacterium gregoryi Argonaute (NgAgo) in human cells. [PDF]
PLoS ONE, 2017 The argonaute protein from the thermophilic bacterium Thermus thermophilus shows DNA-guided DNA interfering activity at high temperatures, complicating its application in mammalian cells.
Parisa Javidi-Parsijani +5 more
doaj +1 more source
Tissue Specific DNA Repair Outcomes Shape the Landscape of Genome Editing
Frontiers in Genetics, 2021 The use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 has moved from bench to bedside in less than 10years, realising the vision of correcting disease through genome editing. The accuracy and safety of this approach relies on
Mathilde Meyenberg +5 more
doaj +1 more source
Translational potential of base-editing tools for gene therapy of monogenic diseases
Frontiers in Bioengineering and Biotechnology, 2022 Millions of people worldwide have rare genetic diseases that are caused by various mutations in DNA sequence. Classic treatments of rare genetic diseases are often ineffective, and therefore great hopes are placed on gene-editing methods.
Vasiliy V. Reshetnikov +7 more
doaj +1 more source
DNA Damage, DNA Repair, and Cancer: Second Edition
International Journal of Molecular Sciences, 2023 Following our first Special Issue, we are pleased to present this Special Issue in the International Journal of Molecular Sciences, titled ‘DNA Damage, DNA Repair, and Cancer: Second Edition’ [...]
openaire +2 more sources
Two steps are better than one: improving gene editing to treat cancer
FEBS Open Bio, 2022 Gene editing enables scientists to make precise changes to the genome of an organism using the cell’s own ability to repair damaged DNA using a supplied DNA template.
Francesca Minter
doaj +1 more source
High-purity production and precise editing of DNA base editing ribonucleoproteins [PDF]
Science Advances, 2021 Cytosine and adenine base editor ribonucleoproteins show precise base editing with reduced DNA and RNA off-target effects.
Hyeon-Ki Jang +9 more
openaire +2 more sources
Stem Cell Research, 2021 Prime editing uses the Cas9 nickase fused to a reverse transcriptase to copy a DNA sequence into a specific locus from a ‘prime editing’ guide RNA (pegRNA), eliminating the need for double-stranded DNA breaks and donor DNA templates.
Nike Bharucha +3 more
doaj +1 more source
RNA Biology, 2022 The ability to precisely alter the genome holds immense potential for molecular biology, medicine and biotechnology. The development of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) into a genomic editing tool has vastly ...
Avantika Ghosh +4 more
doaj +1 more source