Comparison of four DNA extraction and three preservation protocols for the molecular detection and quantification of soil-transmitted helminths in stool [PDF]
PLoS Neglected Tropical Diseases, 2019 Background : A DNA extraction and preservation protocol that yields sufficient and qualitative DNA is pivotal for the success of any nucleic acid amplification test (NAAT), but it still poses a challenge for soil-transmitted helminths (STHs), including ...
Biruksew, Abdissa +9 more
core +2 more sources
A Rapid Bacteriophage DNA Extraction Method [PDF]
Methods and Protocols, 2018 Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria.
Džiuginta Jakočiūnė, A. Moodley
semanticscholar +6 more sources
DNA Extraction and Polymerase Chain Reaction
Journal of Cytology, 2019 DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient ...
N. Gupta
semanticscholar +4 more sources
16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform [PDF]
BMC Microbiology, 2016 peer-reviewedBackground Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the
Claesson, Marcus J. +4 more
core +3 more sources
Mycobacterium avium DNA extraction: Implications for NTM identification and amplicon sequencing [PDF]
Southern African Journal of Infectious DiseasesThis study evaluated six DNA (Deoxyribonucleic acid) extraction methods for Mycobacterium avium, focusing on cost, procedure time, yield, quality, DNA integrity, and suitability for amplicon-based sequencing.
Christoffel J. Opperman +8 more
doaj +2 more sources
Comparison of DNA extraction methods for non-marine molluscs: is modified CTAB DNA extraction method more efficient than DNA extraction kits? [PDF]
3 Biotech, 2019 Isolation of high molecular weight DNA from gastropod molluscs and its subsequent PCR amplification is considered difficult due to excessive mucopolysaccharides secretion which co-precipitate with DNA and obstruct successful amplification.
Sudeshna Chakraborty +2 more
semanticscholar +5 more sources
Isolation and Characterization of Squalene Synthase Gene in Three Species of Achillea, a Rich Source of Saponins [PDF]
Journal of Genetic Resources, 2022 Squalene synthase (SQS, EC.2.5.1.21) is a key enzyme involved in the biosynthesis pathway of triterpenoid and steroidal saponins. The present study aimed to collect molecular information about the SQS gene in Achillea species, the medicinal plants rich ...
Azra Saboora +3 more
doaj +1 more source
Molecular Ecology Resources, 2021 Millions of scientific specimens are housed in museum collections, a large part of which are fluid preserved. The use of formaldehyde as fixative and subsequent storage in ethanol is especially common in ichthyology and herpetology.
N. Straube +10 more
semanticscholar +1 more source
The quality and quantity of compounds affected by viral inactivation methods in dried blood spots
Journal of Laboratory Medicine, 2023 The aim is to evaluate the effect of viral inactivation methods on the quality and quantity of compounds in dried blood spots (DBS).
Wang Ming +5 more
doaj +1 more source
Macedonian Veterinary Review, 2020 The probability of contamination of non-transgenic varieties with genetically modified (GM) products increase as a result of global expansion of areas sown with transgenic crops.
Arita Sabriu-Haxhijaha +3 more
doaj +1 more source