Mycobacterium avium DNA extraction: Implications for NTM identification and amplicon sequencing [PDF]
Southern African Journal of Infectious DiseasesThis study evaluated six DNA (Deoxyribonucleic acid) extraction methods for Mycobacterium avium, focusing on cost, procedure time, yield, quality, DNA integrity, and suitability for amplicon-based sequencing.
Christoffel J. Opperman +8 more
doaj +2 more sources
Isolation and Characterization of Squalene Synthase Gene in Three Species of Achillea, a Rich Source of Saponins [PDF]
Journal of Genetic Resources, 2022 Squalene synthase (SQS, EC.2.5.1.21) is a key enzyme involved in the biosynthesis pathway of triterpenoid and steroidal saponins. The present study aimed to collect molecular information about the SQS gene in Achillea species, the medicinal plants rich ...
Azra Saboora +3 more
doaj +1 more source
A Rapid and Simple Method for DNA Preparation of Magnaporthe oryzae from Single Rice Blast Lesions for PCR-Based Molecular Analysis [PDF]
The Plant Pathology Journal, 2022 Rice blast is one of the most destructive diseases of rice worldwide, and the causative agent is the filamentous ascomycete Magnaporthe oryzae. With the successful cloning of more and more avirulence genes from M.
Liying Dong +6 more
doaj +1 more source
Molecular Ecology Resources, 2021 Millions of scientific specimens are housed in museum collections, a large part of which are fluid preserved. The use of formaldehyde as fixative and subsequent storage in ethanol is especially common in ichthyology and herpetology.
N. Straube +10 more
semanticscholar +1 more source
The quality and quantity of compounds affected by viral inactivation methods in dried blood spots
Journal of Laboratory Medicine, 2023 The aim is to evaluate the effect of viral inactivation methods on the quality and quantity of compounds in dried blood spots (DBS).
Wang Ming +5 more
doaj +1 more source
Comparison of DNA Extraction Methods for Metagenomic Sequencing in Anaerobic Digestion
대한환경공학회지, 2021 Objectives:This study was performed to compare three commercial kits for the extraction of genomic DNA from anaerobic digestate for subsequent iSeq 100 sequencing and microbial community analysis. Methods:A full-scale mesophilic biogas plant was sampled,
Juhee Shin, Youngback Kim, Seung Gu Shin
doaj +1 more source
Macedonian Veterinary Review, 2020 The probability of contamination of non-transgenic varieties with genetically modified (GM) products increase as a result of global expansion of areas sown with transgenic crops.
Arita Sabriu-Haxhijaha +3 more
doaj +1 more source
An improved method for extraction of microbial DNA from alkaline-saline soil
Terra Latinoamericana, 2021 A modified method for the direct extraction of DNA from alkaline-saline soils with minimum DNA fragmentation and a possible reduction in chimera formation during polymerase chain reaction (PCR) was developed. The commercial extraction kit Power Soil DNA (
Valentín Pérez-Hernández +9 more
doaj +1 more source
DNA extraction approaches substantially influence the assessment of the human breast milk microbiome
Scientific Reports, 2020 In addition to providing nutritional and bioactive factors necessary for infant development, human breast milk contains bacteria that contribute to the establishment of commensal microbiota in the infant.
C. Douglas +5 more
semanticscholar +1 more source
Qualitative PCR-based detection of genetically modified soy and maize products in Iran
International Journal of Food Properties, 2020 Soy and maize are the most cultivated genetically modified (GM) crops in the world, which are commonly used in many food products. Establishing regulations for food products containing transgenic materials are obligatory in many countries in order to ...
Payam Safaei +5 more
doaj +1 more source