Isolation and Characterization of Squalene Synthase Gene in Three Species of Achillea, a Rich Source of Saponins [PDF]
Squalene synthase (SQS, EC.2.5.1.21) is a key enzyme involved in the biosynthesis pathway of triterpenoid and steroidal saponins. The present study aimed to collect molecular information about the SQS gene in Achillea species, the medicinal plants rich ...
Azra Saboora+3 more
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A Rapid and Simple Method for DNA Preparation of Magnaporthe oryzae from Single Rice Blast Lesions for PCR-Based Molecular Analysis [PDF]
Rice blast is one of the most destructive diseases of rice worldwide, and the causative agent is the filamentous ascomycete Magnaporthe oryzae. With the successful cloning of more and more avirulence genes from M.
Liying Dong+6 more
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Comparison of DNA Extraction Methods for Metagenomic Sequencing in Anaerobic Digestion
Objectives:This study was performed to compare three commercial kits for the extraction of genomic DNA from anaerobic digestate for subsequent iSeq 100 sequencing and microbial community analysis. Methods:A full-scale mesophilic biogas plant was sampled,
Juhee Shin, Youngback Kim, Seung Gu Shin
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The probability of contamination of non-transgenic varieties with genetically modified (GM) products increase as a result of global expansion of areas sown with transgenic crops.
Arita Sabriu-Haxhijaha+3 more
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The quality and quantity of compounds affected by viral inactivation methods in dried blood spots
The aim is to evaluate the effect of viral inactivation methods on the quality and quantity of compounds in dried blood spots (DBS).
Wang Ming+5 more
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An improved method for extraction of microbial DNA from alkaline-saline soil
A modified method for the direct extraction of DNA from alkaline-saline soils with minimum DNA fragmentation and a possible reduction in chimera formation during polymerase chain reaction (PCR) was developed. The commercial extraction kit Power Soil DNA (
Valentín Pérez-Hernández+9 more
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Qualitative PCR-based detection of genetically modified soy and maize products in Iran
Soy and maize are the most cultivated genetically modified (GM) crops in the world, which are commonly used in many food products. Establishing regulations for food products containing transgenic materials are obligatory in many countries in order to ...
Payam Safaei+5 more
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Superparamagnetic-bead Based Method: An Effective DNA Extraction from Dried Blood Spots (DBS) for Diagnostic PCR [PDF]
Introduction: Storing blood as dried spots on filter paper is a trustworthy approach used in genetic screening issues which justifies the necessity for a reliable DNA extraction method.
Mahmoud Mohammed Sirdah
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Investigation of Bm86 gene analog sequence in some Iranian populations of Hyalomma anatolicum species [PDF]
Ticks are an important problem among zoonotic diseases. Main veterinary species of hard ticks are: Hyalomma sp. Boophilus sp and Rhipicephalus sp. Among them Hyalomma sp. have widespread distribution in Iran and Middle East.
S. Abdollahi+2 more
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Comparison of different DNA extraction methods for the molecular study of bark beetles (Coleoptera: Curculionidae, Scolytinae) [PDF]
Bark beetles are one of the most important pests in forests. Because of their small size and highly similar morphological characters, molecular approaches may be quite useful for a valid species determination.
sudabe Amini+3 more
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