Results 1 to 10 of about 846,282 (296)

Mycobacterium avium DNA extraction: Implications for NTM identification and amplicon sequencing [PDF]

Southern African Journal of Infectious Diseases
This study evaluated six DNA (Deoxyribonucleic acid) extraction methods for Mycobacterium avium, focusing on cost, procedure time, yield, quality, DNA integrity, and suitability for amplicon-based sequencing.
Christoffel J. Opperman, Salim Ben Amor, Greshan Kisten, Brendon C. Mann, Janré Steyn, Sarishna Singh, Yonas Ghebrekristos, Robin Warren, Wynand Goosen   +8 more
doaj   +2 more sources

Isolation and Characterization of Squalene Synthase Gene in Three Species of Achillea, a Rich Source of Saponins [PDF]

Journal of Genetic Resources, 2022
Squalene synthase (SQS, EC.2.5.1.21) is a key enzyme involved in the biosynthesis pathway of triterpenoid and steroidal saponins. The present study aimed to collect molecular information about the SQS gene in Achillea species, the medicinal plants rich ...
Azra Saboora, Maryam Amiri Rad, Ezat Asgarani, Tayebe Rajabian   +3 more
doaj   +1 more source

The quality and quantity of compounds affected by viral inactivation methods in dried blood spots

Journal of Laboratory Medicine, 2023
The aim is to evaluate the effect of viral inactivation methods on the quality and quantity of compounds in dried blood spots (DBS).
Wang Ming, Yu Chaowen, Tang Shi, Liao Zhihong, Wan Kexing, Liu Shan   +5 more
doaj   +1 more source

Genetic Affinity of Echinococcus granulosus protoscolex in Human and Sheep in East Azerbaijan, Iran [PDF]

Archives of Razi Institute, 2020
Echinococcosis caused by the larval form of Echinococcus granulosus (E. granulosus) is known as an important zoonotic disease in various parts of the world, including Iran.
S. Zarrabi Ahrabi, R. Madani, B. Shemshadi, Sh. Ranjbar Bahadori, H. Hashemzadeh Farhang   +4 more
doaj   +1 more source

A modified SDS – based method applied for extraction of high-quality DNA from raw corn and roasted soybean

Macedonian Veterinary Review, 2020
The probability of contamination of non-transgenic varieties with genetically modified (GM) products increase as a result of global expansion of areas sown with transgenic crops.
Arita Sabriu-Haxhijaha, Gordana Ilievska, Velimir Stojkovski, Katerina Blagoevska   +3 more
doaj   +1 more source

Studies on the Rapid and Simple DNA Extraction Method, Antibacterial Activity and Enzyme Activity Involved in Plant Biomass Conversion by Cookeina sulcipes and C. tricholoma (Cup Fungi)

Journal of Pure and Applied Microbiology, 2022
Cookeina sulcipes and C. tricholoma are a cup fungi (Ascomycota) collected in Saraburi, Thailand. The fungi have been isolated, cultured and confirmed as respective species. For morphology, both Cookeina sp.
Narumon Tangthirasunun, Supattra Poeaim
doaj   +1 more source

Detection of the Species of Origin for Pork, Chicken and Beef in Meat Food Products by Real-Time PCR

Safety, 2019
Processed food products of animal origin raise questions related to industrial safety and human health protection. This paper aimed to optimize and validate a real-time, sensitive, and accurate PCR method for the detection and quantification of meat ...
Lavinia-Maria Chiş, Dan Cristian Vodnar   +1 more
doaj   +1 more source

Detection of fire blight resistant genes in apple trees (Malus domestica) planted in Isfahan province using SCAR and SSR markers [PDF]

مجله بیوتکنولوژی کشاورزی, 2020
Objective Plant diseases can be a limiting factor in planting in an area. One of the new methods of producing disease resistant plants is the use of molecular markers.
Marzieh Rabbani, Mohammad Mojtaba Kamelmanesh   +1 more
doaj   +1 more source

Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa)

Journal of Forest Science, 2010
The objective of this work was to apply selected genetic markers to verify the genetic purity of bog pine (Pinus uncinata subsp. uliginosa) individuals from the Borkovicka blata population in order to initiate in vitro cultures from them.
H. Vejsadová, M. Lukášová
doaj   +1 more source

Evaluation of Genetic Diversity in Iranian Violet (Viola spp) Populations Using Morphological and RAPD Molecular Markers [PDF]

Journal of Genetic Resources, 2020
Recognition of genetic reserves and desirable genes is the basis of breeding programs. So far, in Iran, due to the lack of recognition of genetic resources, a considerable breeding program has not been done on native plants.
Samira Abolghasemi, Roohangiz Naderi, Mohammadreza Fattahi Moghadam   +2 more
doaj   +1 more source