Results 1 to 10 of about 4,873,130 (388)
Comparison of four DNA extraction and three preservation protocols for the molecular detection and quantification of soil-transmitted helminths in stool [PDF]
PLoS Neglected Tropical Diseases, 2019 Background : A DNA extraction and preservation protocol that yields sufficient and qualitative DNA is pivotal for the success of any nucleic acid amplification test (NAAT), but it still poses a challenge for soil-transmitted helminths (STHs), including ...
Biruksew, Abdissa +9 more
core +2 more sources
Proceedings of the West Virginia Academy of ScienceKATIE LONG and MARK FLOOD, Forensic Science Program, Fairmont State University, Fairmont, WV 26554. Evaluation of DNA Extraction Methods. Three forms of DNA extraction types include solution-based, column-based, and magnetic bead-based methodologies, with the latter being the accepted methodology used in forensic laboratories. Magnetic bead-based DNA
Katie Long
openaire +2 more sources
Mycobacterium avium DNA extraction: Implications for NTM identification and amplicon sequencing [PDF]
Southern African Journal of Infectious DiseasesThis study evaluated six DNA (Deoxyribonucleic acid) extraction methods for Mycobacterium avium, focusing on cost, procedure time, yield, quality, DNA integrity, and suitability for amplicon-based sequencing.
Christoffel J. Opperman +8 more
doaj +2 more sources
Isolation and Characterization of Squalene Synthase Gene in Three Species of Achillea, a Rich Source of Saponins [PDF]
Journal of Genetic Resources, 2022 Squalene synthase (SQS, EC.2.5.1.21) is a key enzyme involved in the biosynthesis pathway of triterpenoid and steroidal saponins. The present study aimed to collect molecular information about the SQS gene in Achillea species, the medicinal plants rich ...
Azra Saboora +3 more
doaj +1 more source
The American Biology Teacher, 2022 The Next Generation Science Standards (NGSS) were proposed in 2013 in the United States for K–12 learning to emphasize the importance of science and engineering practices in the three dimensions of science learning. In this study, we show that DNA extraction experiments, which are familiar in the United States, can be used not only as demonstration ...
Naoko Kosaka, Yoshisuke Kumano
openaire +1 more source
Applications in Plant Sciences, 2023 Cetyltrimethylammonium bromide (CTAB)–based methods are widely used to isolate DNA from plant tissues, but the unique chemical composition of secondary metabolites among plant species has necessitated optimization.
John J. Schenk +3 more
semanticscholar +1 more source
Scientific Reports, 2023 The gut microbiome is widely analyzed using high-throughput sequencing, such as 16S rRNA gene amplicon sequencing and shotgun metagenomic sequencing (SMS). DNA extraction is known to have a large impact on the metagenomic analyses.
Céline Elie +12 more
semanticscholar +1 more source
The quality and quantity of compounds affected by viral inactivation methods in dried blood spots
Journal of Laboratory Medicine, 2023 The aim is to evaluate the effect of viral inactivation methods on the quality and quantity of compounds in dried blood spots (DBS).
Wang Ming +5 more
doaj +1 more source
Molecular Ecology Resources, 2021 Millions of scientific specimens are housed in museum collections, a large part of which are fluid preserved. The use of formaldehyde as fixative and subsequent storage in ethanol is especially common in ichthyology and herpetology.
N. Straube +10 more
semanticscholar +1 more source
Genes, 2021 The efficient extraction of DNA from challenging samples, such as bones, is critical for the success of downstream genotyping analysis in molecular genetic disciplines. Even though the ancient DNA community has developed several protocols targeting small
C. Xavier +7 more
semanticscholar +1 more source