Results 1 to 10 of about 900,113 (174)

Isolation and Characterization of Squalene Synthase Gene in Three Species of Achillea, a Rich Source of Saponins [PDF]

open access: yesJournal of Genetic Resources, 2022
Squalene synthase (SQS, EC.2.5.1.21) is a key enzyme involved in the biosynthesis pathway of triterpenoid and steroidal saponins. The present study aimed to collect molecular information about the SQS gene in Achillea species, the medicinal plants rich ...
Azra Saboora   +3 more
doaj   +1 more source

A Rapid and Simple Method for DNA Preparation of Magnaporthe oryzae from Single Rice Blast Lesions for PCR-Based Molecular Analysis [PDF]

open access: yesThe Plant Pathology Journal, 2022
Rice blast is one of the most destructive diseases of rice worldwide, and the causative agent is the filamentous ascomycete Magnaporthe oryzae. With the successful cloning of more and more avirulence genes from M.
Liying Dong   +6 more
doaj   +1 more source

Comparison of DNA Extraction Methods for Metagenomic Sequencing in Anaerobic Digestion

open access: yes대한환경공학회지, 2021
Objectives:This study was performed to compare three commercial kits for the extraction of genomic DNA from anaerobic digestate for subsequent iSeq 100 sequencing and microbial community analysis. Methods:A full-scale mesophilic biogas plant was sampled,
Juhee Shin, Youngback Kim, Seung Gu Shin
doaj   +1 more source

A modified SDS – based method applied for extraction of high-quality DNA from raw corn and roasted soybean

open access: yesMacedonian Veterinary Review, 2020
The probability of contamination of non-transgenic varieties with genetically modified (GM) products increase as a result of global expansion of areas sown with transgenic crops.
Arita Sabriu-Haxhijaha   +3 more
doaj   +1 more source

The quality and quantity of compounds affected by viral inactivation methods in dried blood spots

open access: yesJournal of Laboratory Medicine, 2023
The aim is to evaluate the effect of viral inactivation methods on the quality and quantity of compounds in dried blood spots (DBS).
Wang Ming   +5 more
doaj   +1 more source

An improved method for extraction of microbial DNA from alkaline-saline soil

open access: yesTerra Latinoamericana, 2021
A modified method for the direct extraction of DNA from alkaline-saline soils with minimum DNA fragmentation and a possible reduction in chimera formation during polymerase chain reaction (PCR) was developed. The commercial extraction kit Power Soil DNA (
Valentín Pérez-Hernández   +9 more
doaj   +1 more source

Qualitative PCR-based detection of genetically modified soy and maize products in Iran

open access: yesInternational Journal of Food Properties, 2020
Soy and maize are the most cultivated genetically modified (GM) crops in the world, which are commonly used in many food products. Establishing regulations for food products containing transgenic materials are obligatory in many countries in order to ...
Payam Safaei   +5 more
doaj   +1 more source

Superparamagnetic-bead Based Method: An Effective DNA Extraction from Dried Blood Spots (DBS) for Diagnostic PCR [PDF]

open access: yesJournal of Clinical and Diagnostic Research, 2014
Introduction: Storing blood as dried spots on filter paper is a trustworthy approach used in genetic screening issues which justifies the necessity for a reliable DNA extraction method.
Mahmoud Mohammed Sirdah
doaj   +1 more source

Investigation of Bm86 gene analog sequence in some Iranian populations of Hyalomma anatolicum species [PDF]

open access: yesIranian Journal of Animal Biosystematics, 2006
Ticks are an important problem among zoonotic diseases. Main veterinary species of hard ticks are: Hyalomma sp. Boophilus sp and Rhipicephalus sp. Among them Hyalomma sp. have widespread distribution in Iran and Middle East.
S. Abdollahi   +2 more
doaj   +1 more source

Comparison of different DNA extraction methods for the molecular study of bark beetles (Coleoptera: Curculionidae, Scolytinae) [PDF]

open access: yesJournal of Insect Biodiversity and Systematics, 2020
Bark beetles are one of the most important pests in forests. Because of their small size and highly similar morphological characters, molecular approaches may be quite useful for a valid species determination.
sudabe Amini   +3 more
doaj  

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