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DNA Extraction and Polymerase Chain Reaction
DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient ...
N. Gupta
semanticscholar +4 more sources
A Rapid Bacteriophage DNA Extraction Method [PDF]
Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria.
Džiuginta Jakočiūnė, A. Moodley
semanticscholar +6 more sources
New Protocol for DNA Extraction of Stool [PDF]
Present methods for DNA isolation of stool have various limitations such as the amount of stool used, the requirement of lavage fluids or the use of fresh stool. In this paper, a new method is described for the isolation of human nucleic acids from stool, which is independent from the moment of collection.
Barbie M. Machiels+9 more
openalex +5 more sources
Event extraction for DNA methylation [PDF]
We consider the task of automatically extracting DNA methylation events from the biomedical domain literature. DNA methylation is a key mechanism of epigenetic control of gene expression and implicated in many cancers, but there has been little study of automatic information extraction for DNA methylation.We present an annotation scheme for DNA ...
Jun'ichi Tsujii+4 more
openaire +4 more sources
The quality and quantity of compounds affected by viral inactivation methods in dried blood spots
The aim is to evaluate the effect of viral inactivation methods on the quality and quantity of compounds in dried blood spots (DBS).
Wang Ming+5 more
doaj +1 more source
An improved method for extraction of microbial DNA from alkaline-saline soil
A modified method for the direct extraction of DNA from alkaline-saline soils with minimum DNA fragmentation and a possible reduction in chimera formation during polymerase chain reaction (PCR) was developed. The commercial extraction kit Power Soil DNA (
Valentín Pérez-Hernández+9 more
doaj +1 more source
DNA extraction approaches substantially influence the assessment of the human breast milk microbiome
In addition to providing nutritional and bioactive factors necessary for infant development, human breast milk contains bacteria that contribute to the establishment of commensal microbiota in the infant.
C. Douglas+5 more
semanticscholar +1 more source
Qualitative PCR-based detection of genetically modified soy and maize products in Iran
Soy and maize are the most cultivated genetically modified (GM) crops in the world, which are commonly used in many food products. Establishing regulations for food products containing transgenic materials are obligatory in many countries in order to ...
Payam Safaei+5 more
doaj +1 more source
The Evolution of DNA Extraction Methods
Since the first DNA extraction performed by Friedrich Miescher in 1869, scientists have made extraordinary progress in designing extraction methods that are more reliable, easier and faster to perform, more cost-effective and produce a higher yield.
P. Shetty
semanticscholar +1 more source
Investigation of Bm86 gene analog sequence in some Iranian populations of Hyalomma anatolicum species [PDF]
Ticks are an important problem among zoonotic diseases. Main veterinary species of hard ticks are: Hyalomma sp. Boophilus sp and Rhipicephalus sp. Among them Hyalomma sp. have widespread distribution in Iran and Middle East.
S. Abdollahi+2 more
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