Results 11 to 20 of about 900,113 (174)

Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time

open access: yesIndonesian Journal of Halal Research, 2023
DNA (deoxyribonucleic acid) extraction method is the process of separating DNA from the sample. In this process, the DNA obtained must be protected from contamination by RNA, carbohydrates, lipids, and proteins.
Hadi Sunaryo   +5 more
doaj   +1 more source

Overcoming DNA extraction problems from carnivorous plants

open access: yesAnales del Jardín Botánico de Madrid, 2009
We tested previously published protocols for DNA isolation from plants with high contents of polyphenols and polysaccharides for several taxa of carnivorous plants.
Andreas Fleischmann, Günther Heubl
doaj   +1 more source

Optimization of proteinase K incubation protocol duration during DNA extraction from oral squamous cell carcinoma FFPE samples

open access: yesDental Journal, 2023
Background: Formalin-fixed paraffin-embedded (FFPE) specimen archives are a valuable source of sample material for molecular biological analysis. However, the DNA isolated from FFPE samples is usually low in concentration and fragmented.
Asti Meizarini   +3 more
doaj   +1 more source

Detection of fire blight resistant genes in apple trees (Malus domestica) planted in Isfahan province using SCAR and SSR markers [PDF]

open access: yesمجله بیوتکنولوژی کشاورزی, 2020
Objective Plant diseases can be a limiting factor in planting in an area. One of the new methods of producing disease resistant plants is the use of molecular markers.
Marzieh Rabbani   +1 more
doaj   +1 more source

A simple genetic method to distinguish mule deer and bighorn sheep fecal pellets and its application to detecting bighorn sheep colonization events in California

open access: yesCalifornia Fish and Wildlife Journal, 2023
Bighorn sheep (Ovis canadensis) habitat frequently is geographically discontinuous and the metapopulation model fits this species well. Consequently, extinction-colonization dynamics are important and need to be monitored.
John Wehausen
doaj   +1 more source

METHODOLOGICAL ASPECTS OF REAL-TIME PCR USAGE IN ACETOBACTER DETECTION [PDF]

open access: yesJournal of Engineering Science (Chişinău), 2020
Acetic acid bacteria occur in sugar and alcoholised, slightly acid niches such as flowers, fruits, beer, wine, cider, vinegar, souring fruit juices and honey.
BOISTEAN, Alina   +4 more
doaj   +1 more source

Optimized protocol for DNA extraction from ancient skeletal remains using Chelex-100 [PDF]

open access: yes, 2021
PCR-based analysis of skeletonized human remains is a common aspect in both forensic human identification as well as Ancient DNA research. In this, both areas not merely utilize very similar methodology, but also share the same problems regarding quantity and quality of recovered DNA and presence of inhibitory substances in samples from excavated ...
arxiv   +1 more source

Detection of the Species of Origin for Pork, Chicken and Beef in Meat Food Products by Real-Time PCR

open access: yesSafety, 2019
Processed food products of animal origin raise questions related to industrial safety and human health protection. This paper aimed to optimize and validate a real-time, sensitive, and accurate PCR method for the detection and quantification of meat ...
Lavinia-Maria Chiş   +1 more
doaj   +1 more source

Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa)

open access: yesJournal of Forest Science, 2010
The objective of this work was to apply selected genetic markers to verify the genetic purity of bog pine (Pinus uncinata subsp. uliginosa) individuals from the Borkovicka blata population in order to initiate in vitro cultures from them.
H. Vejsadová, M. Lukášová
doaj   +1 more source

Studying DNA translocation in nanocapillaries using single molecule fluorescence [PDF]

open access: yesAppl. Phys. Lett. vol.101, 223704 (2012), 2013
We demonstrate simultaneous measurements of DNA translocation into glass nanopores using ionic current detection and fluorescent imaging. We verify the correspondence between the passage of a single DNA molecule through the nanopore and the accompanying characteristic ionic current blockage.
arxiv   +1 more source

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