METHODOLOGICAL ASPECTS OF REAL-TIME PCR USAGE IN ACETOBACTER DETECTION [PDF]
Journal of Engineering Science (Chişinău), 2020 Acetic acid bacteria occur in sugar and alcoholised, slightly acid niches such as flowers, fruits, beer, wine, cider, vinegar, souring fruit juices and honey.
BOISTEAN, Alina +4 more
doaj +1 more source
A minimally destructive protocol for DNA extraction from ancient teeth
bioRxiv, 2020 Ancient DNA sampling methods—although optimized for efficient DNA extraction—are destructive, relying on drilling or cutting and powdering (parts of) bones and teeth. As the field of ancient DNA has grown, so have concerns about the impact of destructive
Éadaoin Harney +40 more
semanticscholar +1 more source
A Rapid and Simple Method for DNA Preparation of Magnaporthe oryzae from Single Rice Blast Lesions for PCR-Based Molecular Analysis [PDF]
The Plant Pathology Journal, 2022 Rice blast is one of the most destructive diseases of rice worldwide, and the causative agent is the filamentous ascomycete Magnaporthe oryzae. With the successful cloning of more and more avirulence genes from M.
Liying Dong +6 more
doaj +1 more source
Ceramic materials lead to underestimated DNA quantifications : a method for reliable measurements [PDF]
, 2010 In the context of investigating cell-material interactions or of material-guided generation of tissues, DNA quantification represents an elective method to precisely assess the number of cells attached or embedded within different substrates. Nonetheless,
Martin, I., Piccinini, E., Sadr, N.
core +1 more source
Plant Methods, 2020 Background The use of sequencing and genotyping platforms has undergone dramatic improvements, enabling the generation of a wealth of genomic information.
S. Vilanova +10 more
semanticscholar +1 more source
Overcoming DNA extraction problems from carnivorous plants
Anales del Jardín Botánico de Madrid, 2009 We tested previously published protocols for DNA isolation from plants with high contents of polyphenols and polysaccharides for several taxa of carnivorous plants.
Andreas Fleischmann, Günther Heubl
doaj +1 more source
Rapid diagnostic tests for molecular surveillance of Plasmodium falciparum malaria -assessment of DNA extraction methods and field applicability [PDF]
, 2013 Background: The need for new malaria surveillance tools and strategies is critical, given improved global malaria control and regional elimination efforts.
Abdullah S Ali +26 more
core +2 more sources
Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.
BioTechniques, 2013 Procedures utilizing Chelex 100 chelating resin have been developed for extracting DNA from forensic-type samples for use with the PCR. The procedures are simple, rapid, involve no organic solvents and do not require multiple tube transfers for most ...
P. Walsh, D. A. Metzger, Mohammed Taha
semanticscholar +1 more source
Improved Protocol for DNA Extraction from Subsoils Using Phosphate Lysis Buffer
Microorganisms, 2020 As our understanding of soil biology deepens, there is a growing demand for investigations addressing microbial processes in the earth beneath the topsoil layer, called subsoil.
V. Guerra +4 more
semanticscholar +1 more source
Combined DNA extraction and antibody elution from filter papers for the assessment of malaria transmission intensity in epidemiological studies. [PDF]
, 2013 BACKGROUND: Informing and evaluating malaria control efforts relies on knowledge of local transmission dynamics. Serological and molecular tools have demonstrated great sensitivity to quantify transmission intensity in low endemic settings where the ...
Baidjoe, Amrish +13 more
core +3 more sources