A minimally destructive protocol for DNA extraction from ancient teeth
bioRxiv, 2020 Ancient DNA sampling methods—although optimized for efficient DNA extraction—are destructive, relying on drilling or cutting and powdering (parts of) bones and teeth. As the field of ancient DNA has grown, so have concerns about the impact of destructive
Éadaoin Harney +40 more
semanticscholar +1 more source
Ceramic materials lead to underestimated DNA quantifications : a method for reliable measurements [PDF]
, 2010 In the context of investigating cell-material interactions or of material-guided generation of tissues, DNA quantification represents an elective method to precisely assess the number of cells attached or embedded within different substrates. Nonetheless,
Martin, I., Piccinini, E., Sadr, N.
core +1 more source
Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time
Indonesian Journal of Halal Research, 2023 DNA (deoxyribonucleic acid) extraction method is the process of separating DNA from the sample. In this process, the DNA obtained must be protected from contamination by RNA, carbohydrates, lipids, and proteins.
Hadi Sunaryo +5 more
doaj +1 more source
Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.
BioTechniques, 2013 Procedures utilizing Chelex 100 chelating resin have been developed for extracting DNA from forensic-type samples for use with the PCR. The procedures are simple, rapid, involve no organic solvents and do not require multiple tube transfers for most ...
P. Walsh, D. A. Metzger, Mohammed Taha
semanticscholar +1 more source
Combined DNA extraction and antibody elution from filter papers for the assessment of malaria transmission intensity in epidemiological studies. [PDF]
, 2013 BACKGROUND: Informing and evaluating malaria control efforts relies on knowledge of local transmission dynamics. Serological and molecular tools have demonstrated great sensitivity to quantify transmission intensity in low endemic settings where the ...
Baidjoe, Amrish +13 more
core +3 more sources
Detection of the Species of Origin for Pork, Chicken and Beef in Meat Food Products by Real-Time PCR
Safety, 2019 Processed food products of animal origin raise questions related to industrial safety and human health protection. This paper aimed to optimize and validate a real-time, sensitive, and accurate PCR method for the detection and quantification of meat ...
Lavinia-Maria Chiş +1 more
doaj +1 more source
Dental Journal, 2023 Background: Formalin-fixed paraffin-embedded (FFPE) specimen archives are a valuable source of sample material for molecular biological analysis. However, the DNA isolated from FFPE samples is usually low in concentration and fragmented.
Asti Meizarini +3 more
doaj +1 more source
Rapid plant DNA and RNA extraction protocol using a bench drill [PDF]
, 2019 Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after.
Amorim, Edson Perito +11 more
core +3 more sources
California Fish and Wildlife Journal, 2023 Bighorn sheep (Ovis canadensis) habitat frequently is geographically discontinuous and the metapopulation model fits this species well. Consequently, extinction-colonization dynamics are important and need to be monitored.
John Wehausen
doaj +1 more source
Detection of fire blight resistant genes in apple trees (Malus domestica) planted in Isfahan province using SCAR and SSR markers [PDF]
مجله بیوتکنولوژی کشاورزی, 2020 Objective Plant diseases can be a limiting factor in planting in an area. One of the new methods of producing disease resistant plants is the use of molecular markers.
Marzieh Rabbani +1 more
doaj +1 more source