Assessment of fecal DNA extraction protocols for metagenomic studies
GigaScience, 2020 Background Shotgun metagenomic sequencing has improved our understanding of the human gut microbiota. Various DNA extraction methods have been compared to find protocols that robustly and most accurately reflect the original microbial community ...
Fangming Yang +13 more
semanticscholar +1 more source
Overcoming DNA extraction problems from carnivorous plants
Anales del Jardín Botánico de Madrid, 2009 We tested previously published protocols for DNA isolation from plants with high contents of polyphenols and polysaccharides for several taxa of carnivorous plants.
Andreas Fleischmann, Günther Heubl
doaj +1 more source
Detection of fire blight resistant genes in apple trees (Malus domestica) planted in Isfahan province using SCAR and SSR markers [PDF]
مجله بیوتکنولوژی کشاورزی, 2020 Objective Plant diseases can be a limiting factor in planting in an area. One of the new methods of producing disease resistant plants is the use of molecular markers.
Marzieh Rabbani +1 more
doaj +1 more source
Rapid diagnostic tests for molecular surveillance of Plasmodium falciparum malaria -assessment of DNA extraction methods and field applicability [PDF]
, 2013 Background: The need for new malaria surveillance tools and strategies is critical, given improved global malaria control and regional elimination efforts.
Abdullah S Ali +26 more
core +2 more sources
Detection of the Species of Origin for Pork, Chicken and Beef in Meat Food Products by Real-Time PCR
Safety, 2019 Processed food products of animal origin raise questions related to industrial safety and human health protection. This paper aimed to optimize and validate a real-time, sensitive, and accurate PCR method for the detection and quantification of meat ...
Lavinia-Maria Chiş +1 more
doaj +1 more source
Qualitative PCR-based detection of genetically modified soy and maize products in Iran
International Journal of Food Properties, 2020 Soy and maize are the most cultivated genetically modified (GM) crops in the world, which are commonly used in many food products. Establishing regulations for food products containing transgenic materials are obligatory in many countries in order to ...
Payam Safaei +5 more
doaj +1 more source
Combined DNA extraction and antibody elution from filter papers for the assessment of malaria transmission intensity in epidemiological studies. [PDF]
, 2013 BACKGROUND: Informing and evaluating malaria control efforts relies on knowledge of local transmission dynamics. Serological and molecular tools have demonstrated great sensitivity to quantify transmission intensity in low endemic settings where the ...
Baidjoe, Amrish +13 more
core +3 more sources
DNA extraction approaches substantially influence the assessment of the human breast milk microbiome
Scientific Reports, 2020 In addition to providing nutritional and bioactive factors necessary for infant development, human breast milk contains bacteria that contribute to the establishment of commensal microbiota in the infant.
C. Douglas +5 more
semanticscholar +1 more source
Journal of Forest Science, 2010 The objective of this work was to apply selected genetic markers to verify the genetic purity of bog pine (Pinus uncinata subsp. uliginosa) individuals from the Borkovicka blata population in order to initiate in vitro cultures from them.
H. Vejsadová, M. Lukášová
doaj +1 more source
Point of Care Diagnosis of Multiple Schistosome Parasites: Species-specific DNA Detection in Urine by Loop-mediated Isothermal Amplification (LAMP) [PDF]
, 2017 Schistosomes are easily transmitted and high chance of repeat infection, so if control strategies based on targeted mass drug administration (MDA) are to succeed it is essential to have a test that is sensitive, accurate and simple to use.
Anyan, William K. +6 more
core +2 more sources