Results 41 to 50 of about 846,282 (296)

Robust CTAB-activated charcoal protocol for plant DNA extraction

Acta Agriculturae Slovenica, 2006
DNA extracted from plants rich in polyphenols and/or polysaccharides is often problematic when subjected to polymerase chain reaction, especially when mature tissues are used for DNA extraction.
Mitja KRIŽMAN, Jernej JAKŠE, Dea BARIČEVIČ, Branka JAVORNIK, Mirko PROŠEK   +4 more
doaj   +1 more source

Extracellular DNA extraction v1 [PDF]

, 2020
Over the past decade, an increasing number of studies has used environmental DNA from lake sediments to trace past lake ecosystem and landscape changes, agricultural activities or human presence and more broadly the biodiversity. In the environment, DNA can be found as intracellular and extracellular DNA (iDNA and exDNA).
Charline Giguet-Covex, Pierre Taberlet, Francesco Gentile Ficetola   +2 more
openaire   +2 more sources

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

FEBS Letters, EarlyView.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan, Victoria Scarpelli, Marten Exterkate   +2 more
wiley   +1 more source

ISOLATION AND IDENTIFICATION OF ALOCALSTRAIN OF PROBIOTIC BACTERIAL ACTOBACILLUS PLANTARUM AND STUDIED THE TOLERANCE ABILITY FOR DIFFERENT LEVELS OF pH [PDF]

Basrah Journal of Veterinary Research, 2016
Lactobacillus plantarum IRQ12 was isolated from local pickle producing from Cucumis sativus submerged in 5% salt solution for one week far from sun light, selective media was used for isolation and activation process which was MRS broth and MRS agar ...
Sinan J. Abdul-Abbas, Adnan E. Al-Badran, Hasan R. Al-Sherifi, Aswan H. Aboud Al-bayyar   +3 more
doaj   +1 more source

C‐mannosylation promotes ADAMTS1 activation and secretion in human testicular germ cell tumor NEC8 cells

FEBS Letters, EarlyView.
C‐mannosylation is a unique form of protein glycosylation. In this study, we demonstrated that ADAMTS1 is C‐mannosylated at Trp562 and Trp565 in human testicular germ cell tumor NEC8 cells. We found that C‐mannosylation of ADAMTS1 is essential for its secretion, processing, enzymatic activity, and ability to promote vasculogenic mimicry. These findings
Takato Kobayashi, Takehiro Suzuki, Ryota Kawahara, Natsumi Harai, Naoshi Dohmae, Siro Simizu   +5 more
wiley   +1 more source

Study of the DNA Extraction from the Nail by Spin Column-based Nucleic Acid Purification

Journal of Forensic Science and Medicine
Background: Nails are one of the objects that are more durable than other witness objects. This study was interested in whether the Spin column-based nucleic acid purification technique could extract DNA from nails to give satisfactory results.
Kanchana Sujirachato, Piya Wongyanin, Pilaiwan Ramadjai, Alisa Ladsuk, Kingkan Pokkasap, Wisarn Worasuwannarak   +5 more
doaj   +1 more source

Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction [PDF]

BMC Research Notes, 2014
There are challenges, when extracting bacterial DNA from specimens for molecular diagnostics, since fecal samples also contain DNA from human cells and many different substances derived from food, cell residues and medication that can inhibit downstream PCR.
Lee O’Brien Andersen, Karen A. Krogfelt, Carsten Struve, Andreas Petersen, Søren Persson, Hengameh Mirsepasi   +5 more
openaire   +3 more sources

The multidrug and toxin extrusion (MATE) transporter DTX51 antagonizes non‐cell‐autonomous HLS1–AMP1 signaling in a region‐specific manner

FEBS Letters, EarlyView.
The Arabidopsis mutants hls1 hlh1 and amp1 lamp1 exhibit pleiotropic developmental phenotypes. Although the functions of the causative genes remain unclear, they act in the same genetic pathway and are thought to generate non‐cell‐autonomous signals.
Takashi Nobusawa, Makoto Kusaba
wiley   +1 more source

Ergothioneine supplementation improves pup phenotype and survival in a murine model of spinal muscular atrophy

FEBS Letters, EarlyView.
Spinal muscular atrophy (SMA) is a genetic disease affecting motor neurons. Individuals with SMA experience mitochondrial dysfunction and oxidative stress. The aim of the study was to investigate the effect of an antioxidant and neuroprotective substance, ergothioneine (ERGO), on an SMNΔ7 mouse model of SMA.
Francesca Cadile, Daniela Ratto, Giorgia Rastelli, Ottavia Eleonora Ferraro, Caterina Temporini, Sunil Kumar, Simona Boncompagni, Paola Rossi, Monica Canepari   +8 more
wiley   +1 more source

Dental Floss-derived Biological Sample Collection， DNA Extraction and STR Typing

Fayixue Zazhi
ObjectiveTo evaluate the forensic application value of used dental floss as a source of biological evidence for individual identification by analyzing the effects of dental floss sample collection methods, DNA extraction methods, preservation conditions,
LI Ze-qin, YUAN Fang, LIU Na, YAN Jiang-wei, ZHANG Geng-qian   +4 more
doaj   +1 more source