Comparison of different DNA extraction methods for the molecular study of bark beetles (Coleoptera: Curculionidae, Scolytinae) [PDF]
Bark beetles are one of the most important pests in forests. Because of their small size and highly similar morphological characters, molecular approaches may be quite useful for a valid species determination.
sudabe Amini+3 more
doaj
ISOLATION AND IDENTIFICATION OF ALOCALSTRAIN OF PROBIOTIC BACTERIAL ACTOBACILLUS PLANTARUM AND STUDIED THE TOLERANCE ABILITY FOR DIFFERENT LEVELS OF pH [PDF]
Lactobacillus plantarum IRQ12 was isolated from local pickle producing from Cucumis sativus submerged in 5% salt solution for one week far from sun light, selective media was used for isolation and activation process which was MRS broth and MRS agar ...
Sinan J. Abdul-Abbas+3 more
doaj +1 more source
Cookeina sulcipes and C. tricholoma are a cup fungi (Ascomycota) collected in Saraburi, Thailand. The fungi have been isolated, cultured and confirmed as respective species. For morphology, both Cookeina sp.
Narumon Tangthirasunun, Supattra Poeaim
doaj +1 more source
Evaluation of Genetic Diversity in Iranian Violet (Viola spp) Populations Using Morphological and RAPD Molecular Markers [PDF]
Recognition of genetic reserves and desirable genes is the basis of breeding programs. So far, in Iran, due to the lack of recognition of genetic resources, a considerable breeding program has not been done on native plants.
Samira Abolghasemi+2 more
doaj +1 more source
The Impact of DNA Extraction Methods on Stool Bacterial and Fungal Microbiota Community Recovery
Our understanding of human gut microbiota in health and disease depends on accurate and reproducible microbial data acquisition. The critical step in this process is to apply an appropriate methodology to extract microbial DNA, since biases introduced ...
K. Fiedorová+8 more
semanticscholar +1 more source
Genetic Affinity of Echinococcus granulosus protoscolex in Human and Sheep in East Azerbaijan, Iran [PDF]
Echinococcosis caused by the larval form of Echinococcus granulosus (E. granulosus) is known as an important zoonotic disease in various parts of the world, including Iran.
S. Zarrabi Ahrabi+4 more
doaj +1 more source
DNA extraction for human microbiome studies: the issue of standardization
Among the laboratory and bioinformatic processing steps for human microbiome studies, a lack of consistency in DNA extraction methodologies is hindering the ability to compare results between studies and sometimes leading to errant conclusions.
K. Greathouse, R. Sinha, E. Vogtmann
semanticscholar +1 more source
Optimization of DNA extraction methods for genomic analysis of rice root-knot nematode (Meloidogyne graminicola) using PCR (polymerase chain reaction) and sanger sequencing [PDF]
The root-knot nematode Meloidogyne graminicola is an economically important pest in rice production. The identification of a nematode species is an important basis in nematode management to reduce yield losses by extracting nematode DNA as an early step ...
Rendyta Morindya+3 more
doaj +1 more source
BackgroundHepatitis B virus (HBV) infection is the major public health problem worldwide. In clinical practice, serological and molecular assays are the most commonly used diagnostic methods to detect HBV infection in clinical practices.MethodsHere we ...
X. Shen+10 more
semanticscholar +1 more source
DNA extraction from formalin-fixed Franciscana tissues [PDF]
The present paper reports the extraction of DNA from formalin-fixed Pontoporia blainvillei tissues. Following the Vachot and Monerot (1996) protocol, fragmented DNA (300-700bp) was extracted from more than 95% of liver and muscle samples.
Bastida, R.+2 more
core +2 more sources