Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize
Plant Methods, 2017 The world’s top three cereals, based on their monetary value, are rice, wheat, and corn. In cereal crops, DNA extraction is difficult owing to rigid non-cellulose components in the cell wall of leaves and high starch and protein content in grains.
A. Abdel-latif, G. Osman
semanticscholar +1 more source
Using eggshell membranes as a DNA source for population genetic research [PDF]
, 2009 In the context of population genetic research, a faster and less invasive method of DNA sampling would allow large-scale assessments of genetic diversity and genetic differentiation with the help of volunteer observers.
Broekman, J. +4 more
core +3 more sources
Comparison of methods to extract PCR-amplifiable DNA from fruit, herbal and black teas
Czech Journal of Food Sciences, 2021 The success of polymerase chain reaction (PCR) assay depends on template deoxyribonucleic acid (DNA) being sufficient with respect to both quantity and quality.
Eliška Čermáková +3 more
doaj +1 more source
A modified SDS-based DNA extraction method from raw soybean
Bioscience Reports, 2019 Soybean is the most important genetically modified (GM) oilseed worldwide. Regulations relating to the approval of biotech soybean varieties and product labeling demand accurate and reliable detection techniques to screen for GM soya.
Yi-miao Xia +6 more
semanticscholar +1 more source
Insights into PI3K/AKT signaling in B cell development and chronic lymphocytic leukemia
FEBS Letters, EarlyView.This Review explores how the phosphoinositide 3‐kinase and protein kinase B pathway shapes B cell development and drives chronic lymphocytic leukemia, a common blood cancer. It examines how signaling levels affect disease progression, addresses treatment challenges, and introduces novel experimental strategies to improve therapies and patient outcomes.
Maike Buchner
wiley +1 more source
Introducing automation to the molecular diagnosis of Trypanosoma cruzi infection: A comparative study of sample treatments, DNA extraction methods and real-time PCR assays [PDF]
, 2018 Background Polymerase chain reaction (PCR) has become a useful tool for the diagnosis of Trypanosoma cruzi infection. The development of automated DNA extraction methodologies and PCR systems is an important step toward the standardization of protocols ...
Abras, Alba +12 more
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Simplified DNA Extraction Methods for Sexing Chick Embryos
The Journal of Poultry Science, 2004 We investigated the efficiency of DNA extraction and the accuracy of polymerase chain reaction- (PCR-) based sexing of DNA that was obtained from adult chickens and early chicken embryos. DNA samples were prepared using water-, ammonium-, and proteinase-
Takeo Minematsu +4 more
doaj +1 more source
Use of FFPE-derived DNA in next generation sequencing: DNA extraction methods
bioRxiv, 2019 Archival tissues represent a rich resource for clinical genomic studies, particularly when coupled with comprehensive medical records. Use of these in NGS is a priority.
S. McDonough +7 more
semanticscholar +1 more source
Taurine promotes glucagon‐like peptide‐1 secretion in enteroendocrine L cells
FEBS Letters, EarlyView.Taurine, a sulfur‐containing amino acid, is likely taken up by enteroendocrine L cells via the taurine transporter. This process increases the levels of cytosolic ATP. The increase in intracellular Ca2+ concentrations and glucagon‐like peptide‐1 secretion through membrane depolarization is caused by the closure of ATP‐sensitive potassium channels ...
Yuri Osuga +6 more
wiley +1 more source
An efficient protocol to perform genetic traceability of tissue and foods from Geoffroea decorticans
, 2019 The quality of a DNA isolation method depends, among others, on the target tissue and the metabolites therein. Geoffroea decorticans Burkart (chanar) is a species that has nutritional and pharmacological potential.
Aguayo, F. +3 more
core +1 more source