Results 61 to 70 of about 4,873,130 (388)

Determination of plasmid copy number in yeast transformants by means of agarose plugs [PDF]

open access: yes, 1993
The determination of plasmid copy number in Saccharomyces cerevisiaetransformants containing circular or linear plasmids is currently performed with total yeast DNA extracts obtained from cultures grown under selection.
AM Guerrini   +3 more
core   +1 more source

Organoids in pediatric cancer research

open access: yesFEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Study of the DNA Extraction from the Nail by Spin Column-based Nucleic Acid Purification

open access: yesJournal of Forensic Science and Medicine
Background: Nails are one of the objects that are more durable than other witness objects. This study was interested in whether the Spin column-based nucleic acid purification technique could extract DNA from nails to give satisfactory results.
Kanchana Sujirachato   +5 more
doaj   +1 more source

Molecular characterization of rice (Oryza sativa L.) genotypes for drought tolerance using two SSR markers

open access: yesElectronic Journal of Plant Breeding, 2017
Plant growth as well as productivity of rice (Oryza sativa L.), the major cereal and staple food of millions, is significantly affected by numerous biotic and abiotic stresses.
Shaheen A. Salam   +3 more
doaj   +1 more source

An improved method for extraction of microbial DNA from alkaline-saline soil

open access: yesTerra Latinoamericana, 2021
A modified method for the direct extraction of DNA from alkaline-saline soils with minimum DNA fragmentation and a possible reduction in chimera formation during polymerase chain reaction (PCR) was developed. The commercial extraction kit Power Soil DNA (
Valentín Pérez-Hernández   +9 more
doaj   +1 more source

Following basal stem rot in young oil palm plantings [PDF]

open access: yes, 2005
The PCR primer GanET has previously been shown to be suitable for the specific amplification of DNA from Ganoderma boninense. A DNA extraction and PCR method has been developed that allows for the amplification of the G.
CA Levesque   +17 more
core   +1 more source

Use of FFPE-derived DNA in next generation sequencing: DNA extraction methods

open access: yesbioRxiv, 2019
Archival tissues represent a rich resource for clinical genomic studies, particularly when coupled with comprehensive medical records. Use of these in NGS is a priority.
S. McDonough   +7 more
semanticscholar   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

open access: yesFEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Superparamagnetic-bead Based Method: An Effective DNA Extraction from Dried Blood Spots (DBS) for Diagnostic PCR [PDF]

open access: yesJournal of Clinical and Diagnostic Research, 2014
Introduction: Storing blood as dried spots on filter paper is a trustworthy approach used in genetic screening issues which justifies the necessity for a reliable DNA extraction method.
Mahmoud Mohammed Sirdah
doaj   +1 more source

A rapid and sensitive recombinase aided amplification assay to detect hepatitis B virus without DNA extraction

open access: yesBMC Infectious Diseases, 2019
BackgroundHepatitis B virus (HBV) infection is the major public health problem worldwide. In clinical practice, serological and molecular assays are the most commonly used diagnostic methods to detect HBV infection in clinical practices.MethodsHere we ...
X. Shen   +10 more
semanticscholar   +1 more source

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