Results 61 to 70 of about 371,482 (299)

Optimization of enzymatic fragmentation is crucial to maximize genome coverage: a comparison of library preparation methods for Illumina sequencing

BMC Genomics, 2022
Background Novel commercial kits for whole genome library preparation for next-generation sequencing on Illumina platforms promise shorter workflows, lower inputs and cost savings. Time savings are achieved by employing enzymatic DNA fragmentation and by
Teodora Ribarska, Pål Marius Bjørnstad, Arvind Y. M. Sundaram, Gregor D. Gilfillan   +3 more
doaj   +1 more source

A High-Throughput Method for Illumina RNA-Seq Library Preparation. [PDF]

, 2012
With the introduction of cost effective, rapid, and superior quality next generation sequencing techniques, gene expression analysis has become viable for labs conducting small projects as well as large-scale gene expression analysis experiments. However,
Daniel H Chitwood, Jie ePeng, Julin N Maloof, Lauren R Headland, Neelima R Sinha, Ravi eKumar, Seisuke eKimura, Seisuke eKimura, Yasunori eIchihashi   +8 more
core   +2 more sources

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Study Protocol: Comparison of Clinical Pregnancy Outcomes of Intracytoplasmic Sperm Injection (ICSI) Using Testicular and Ejaculated Spermatozoa in Male Patients with High DNA Fragmentation

Journal of Pharmacy and Bioallied Sciences
Background: This study aims to compare the clinical pregnancy outcome of intracytoplasmic sperm injection (ICSI) using testicular and ejaculated spermatozoa in male patients with high deoxyribonucleic acid (DNA) fragmentation.
Juhi Barai, Akash More, Namrata Choudhary   +2 more
doaj   +1 more source

Deterioration of willow seeds during storage [PDF]

, 2018
Willow (Salix spp.) seeds are able to tolerate desiccation, but differ from typical orthodox seeds in that they lose viability in a few days at room temperature, and in that the chloroplasts in embryo tissues do not dedifferentiate during maturation ...
Burrieza, Hernán Pablo, Correa, María Daniela, Lopez Fernandez, Maria Paula, Maldonado, Sara Beatriz, Moyano, Laura, Vasile, Franco Emanuel   +5 more
core   +2 more sources

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing.

PLoS ONE, 2019
Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of ...
Konstantin B Ignatov, Konstantin A Blagodatskikh, Dmitry S Shcherbo, Tatiana V Kramarova, Yulia A Monakhova, Vladimir M Kramarov   +5 more
doaj   +1 more source

Features of structure and dynamics in damages of fertile properties in ejaculate under the influence of toxocariasis invasion

Сучасні медичні технології, 2020
Purpose of the study. To study the features of changes of spermiologic values and degree of spermatozoa DNA fragmentation depending on the presence/absence of toxocariasis invasion. Materials and methods.
L. L. Voroncova, S. O. Kenijz, V. A. Kovalenko   +2 more
doaj   +1 more source

A new model for ancient DNA decay based on paleogenomic meta-analysis [PDF]

, 2017
The persistence of DNA over archaeological and paleontological timescales in diverse environments has led to a revolutionary body of paleogenomic research, yet the dynamics of DNA degradation are still poorly understood.
Allentoft, Allentoft, Briggs, Campos, Cassidy, Dabney, Deagle, Demarchi, Dodt, Enk, Frantz, Gallego Llorente, Gamba, Gilbert, Grass, Green, Günther, Haak, Haber, Hansen, Hijmans, Hijmans, Hofman, Hofmanová, Jeong, Jónsson, Keller, Kistler, Kistler, Koslicki, Leonardi, Li, Li, Librado, Logan Kistler, Lynch, Martiniano, Matthew Collins, Miller, Olalde, Olalde, Oliver Smith, Orlando, Orlando, Ornstein, Ottoni, Paabo, Palkopoulou, Palmer, Park, Pedersen, Plummer, Poinar, Pääbo, R Development Core Team, Rasmussen, Rasmussen, Robin G. Allaby, Roselyn Ware, Sawyer, Schmitt, Schubert, Shapiro, Skoglund, Skoglund, Smith, Wade, Wales, Wang, Warinner, Weiß, Yoshida, Zhang   +72 more
core   +4 more sources

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source