Structure of Formamidopyrimidine-DNA Glycosylase Covalently Complexed to DNA [PDF]
Journal of Biological Chemistry, 2002 Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme that excises oxidized purines from damaged DNA. The Schiff base intermediate formed during this reaction between Escherichia coli Fpg and DNA was trapped by reduction with sodium borohydride, and the structure of the resulting covalently cross-linked complex was determined at a 2.1-A ...
R. Gilboa +9 more
openaire +3 more sources
Mechanism of Werner DNA helicase: POT1 and RPA stimulates WRN to unwind beyond gaps in the translocating strand. [PDF]
PLoS ONE, 2009 WRN belongs to the RecQ family of DNA helicases and it plays a role in recombination, replication, telomere maintenance and long-patch base excision repair. Here, we demonstrate that WRN efficiently unwinds DNA substrates containing a 1-nucleotide gap in
Byungchan Ahn +4 more
doaj +1 more source
The DNA Repair Protein XRCC1 Functions in the Plant DNA Demethylation Pathway by Stimulating Cytosine Methylation (5-meC) Excision, Gap Tailoring, and DNA Ligation [PDF]
, 2013 Background: Active DNA demethylation in plants is initiated by 5-meC DNA glycosylases of the ROS1/DME family and continued through a base excision repair pathway.
Córdoba-Cañero, Dolores +3 more
core +1 more source
Uracil-DNA glycosylase as a probe for protein-DNA interactions [PDF]
Nucleic Acids Research, 1993 The DNA repair enzyme Uracil-DNA Glycosylase (UDG) can be used to investigate three different features of protein-DNA interactions. Complexes can be probed by simple protection experiments ('footprinting') or by two kinds of interference assays: a missing thymine site (MT-site) experiment and a missing thymine methyl site (MTM-site) experiment.
Pallavi R. Devchand +2 more
openaire +3 more sources
An Update: Enzymatic Synthesis for Industrial Applications
Angewandte Chemie International Edition, EarlyView.Biocatalysis has become a sustainable and cost‐competitive alternative to established chemical synthesis, enabling the enzyme‐based production of not only commodity chemicals but (non‐natural) amino acids, (rare) sugars, as well as synthetic nucleotides.
Thomas Bayer +4 more
wiley +1 more source
Chinese Medical Journal, 2016 Background: Ginsenoside Rd (GSRd), one of the main active ingredients in traditional Chinese herbal Panax ginseng, has been found to have therapeutic effects on ischemic stroke.
Long-Xiu Yang, Xiao Zhang, Gang Zhao
doaj +1 more source
Excision of damaged bases from transcription intermediates by FPG/NEI superfamily DNA glycosylases [PDF]
, 2007 Oxidative lesions are abundant due to constant presence of reactive oxygen species in living cells. Repair of oxidative base lesions is initiated by DNA glycosylases.
Amon, Peter +4 more
core +2 more sources
A universal framework to efficiently share material and process resources in the DNA construction world [PDF]
arXiv, 2021 DNA constructs and their annotated sequence maps have been rapidly accumulating with the advancement of DNA cloning, synthesis, and assembly methods. Such a resource has the potential to be optimally utilized in an autonomous DNA building platform. However, most DNA design processes today remain manually operated with the assistance of graphical user ...
arxiv
ATP-Stimulated, DNA-Mediated Redox Signaling by XPD, a DNA Repair and Transcription Helicase [PDF]
, 2011 Using DNA-modified electrodes, we show DNA-mediated signaling by XPD, a helicase that contains a [4Fe-4S] cluster and is critical for nucleotide excision repair and transcription.
Barton, Jacqueline K. +4 more
core +1 more source
Frameshift Mutagenesis and Microsatellite Instability Induced by Human Alkyladenine DNA Glycosylase [PDF]
, 2009 Human alkyladenine DNA glycosylase (hAAG) excises alkylated purines, hypoxanthine, and etheno bases from DNA to form abasic (AP) sites. Surprisingly, elevated expression of hAAG increases spontaneous frameshift mutagenesis. By random mutagenesis of eight
Guo, Haiwei H. +6 more
core +1 more source