Results 91 to 100 of about 2,038,987 (238)

Cas9‐Embedding Hyperactive TadA8e Confers Efficient and Highly Specific A‐To‐G Base Editing in Rice

Plant Biotechnology Journal, EarlyView.
ABSTRACT Adenine base editors (ABEs) produce precise A‐to‐G conversion in the genomic target sites without causing double‐strand breaks. However, the hyperactive adenosine deaminase TadA8e raises safety concerns on genome‐wide off‐target edits. We engineered 11 chimeric proteins for ABEs (CP‐ABEs) by embedding hyperactive TadA8e within Cas9 nickase to ...
Jianjian Hu, Xue Li, Yuhong Gao, Yifan Guo, Yini Liu, Chen Wang, Gencheng Xu, Chaoyue Du, Shijia Liu, Zhigang Zhao, Yihua Wang, Yufeng Wu, Xiaoou Dong, Chao Li, Jianmin Wan   +14 more
wiley   +1 more source

Utility of blood as the clinical specimen for the diagnosis of ocular toxoplasmosis using uracil DNA glycosylase-supplemented loop-mediated isothermal amplification and real-time polymerase chain reaction assays based on REP-529 sequence and B1 gene [PDF]

, 2022
Bahman Rahimi Esboei, Shirzad Fallahi, Mohammad Zarei, Bahram Kazemi, Mehdi Mohebali, Saeedeh Shojaee, Parisa Mousavi, Aref Teimouri, Raziyeh Mahmoudzadeh, Mirataollah Salabati, Hossein Keshavarz Valian   +10 more
openalex   +1 more source

8-Oxoguanine DNA Glycosylases: One Lesion, Three Subfamilies

International Journal of Molecular Sciences, 2012
Amongst the four bases that form DNA, guanine is the most susceptible to oxidation, and its oxidation product, 7,8-dihydro-8-oxoguanine (8-oxoG) is the most prevalent base lesion found in DNA. Fortunately, throughout evolution cells have developed repair
F. Faucher, S. Doublié, Z. Jia
semanticscholar   +1 more source

[Uracil-DNA glycosylases].

Postepy biochemii, 2009
Uracil is one of four nitrogen bases, most frequently found in normal RNA. Uracyl can be found also in DNA as a result of enzymatic or non-enzymatic deamination of cytosine as well as misincorporation of dUMP instead of dTMP during DNA replication. Uracil from DNA can be removed by DNA repair enzymes with apirymidine site as an intermediate.
Dariusz, Pytel, Artur, Słupianek, Dominika, Ksiazek, Tomasz, Skórski, Janusz, Błasiak   +4 more
openaire   +1 more source

The PagDMG6341–PagWD40–PagPOLD4 Module Coordinates Base Excision Repair in ‘84K’ Poplar (Populus alba × P. glandulosa)

Plant Biotechnology Journal, EarlyView.
ABSTRACT Base excision repair (BER) is a critical pathway for repairing damaged DNA bases in cells; however, the mechanisms of protein recruitment and interaction in this pathway remain largely unexplored in higher plants. In this study, we used ‘84K’ poplar (Populus alba × P.
Aoyu Ling, Yijia Jin, Yufei Xia, Shenxiu Jiang, Jianghai Shu, Xiaotong Hu, Kang Du, Pingdong Zhang, Xiangyang Kang   +8 more
wiley   +1 more source

Approaches to Detection of Distantly Related Proteins by Database Searches

BioTechniques, 1996
The searching of protein databases as a method of identifying newly sequenced genes is commonplace in molecular biology laboratories. However, it is a procedure that is not usually formally taught to students, and method cookbooks discuss it only briefly.
K. Cattell, B. Koop, R.S. Olafson, M. Fellows, I. Bailey, R.W. Olafson, C. Upton   +6 more
doaj   +1 more source

7,8-dihydro-8-oxoadenine, a highly mutagenic adduct, is repaired by Escherichia coli and human mismatch-specific uracil/thymine-DNA glycosylases

Nucleic Acids Research, 2012
Hydroxyl radicals predominantly react with the C8 of purines forming 7,8-dihydro-8-oxoguanine (8oxoG) and 7,8-dihydro-8-oxoadenine (8oxoA) adducts, which are highly mutagenic in mammalian cells.
Ibtissam Talhaoui, S. Couvé, A. Ishchenko, Christophe Kunz, P. Schär, M. Saparbaev   +5 more
semanticscholar   +1 more source

A stage‐dependent seed defense response to explain efficient seed transmission of Xanthomonas citri pv. fuscans to common bean

Plant, Cell &Environment, EarlyView.
Abstract Although seed represents an important means of plant pathogen dispersion, the seed–pathogen dialogue remains largely unexplored. A multiomic approach was performed at different seed developmental stages of common bean (Phaseolus vulgaris L.) during asymptomatic colonization by Xanthomonas citri pv.
Armelle Darrasse, Łukasz Paweł Tarkowski, Martial Briand, David Lalanne, Nicolas W. G. Chen, Matthieu Barret, Jerome Verdier   +6 more
wiley   +1 more source

The Influence of (5′R)- and (5′S)-5′,8-Cyclo-2′-Deoxyadenosine on UDG and hAPE1 Activity. Tandem Lesions are the Base Excision Repair System’s Nightmare

Cells, 2019
DNA lesions are formed continuously in each living cell as a result of environmental factors, ionisation radiation, metabolic processes, etc. Most lesions are removed from the genome by the base excision repair system (BER).
Bolesław T. Karwowski
doaj   +1 more source

DNA Hypomethylation Is One of the Epigenetic Mechanisms Involved in Salt‐Stress Priming in Soybean Seedlings

Plant, Cell &Environment, EarlyView.
ABSTRACT Salt‐stress priming enhances the tolerance of plants against subsequent exposure to a similar stress. Priming‐induced transcriptomic reprogramming is mediated by multiple epigenetic mechanisms, the best known of which is histone modifications. However, not much is known about other epigenetic responses.
Wai‐Shing Yung, Qianwen Wang, Long‐Yiu Chan, Zhili Wang, Mingkun Huang, Man‐Wah Li, Fuk‐Ling Wong, Hon‐Ming Lam   +7 more
wiley   +1 more source