Results 81 to 90 of about 2,038,987 (238)

Zinc finger oxidation of Fpg/Nei DNA glycosylases by 2-thioxanthine: biochemical and X-ray structural characterization

Nucleic Acids Research, 2014
DNA glycosylases from the Fpg/Nei structural superfamily are base excision repair enzymes involved in the removal of a wide variety of mutagen and potentially lethal oxidized purines and pyrimidines.
A. Biela, F. Coste, F. Culard, M. Guérin, S. Goffinont, Karola L. Gasteiger, J. Cieśla, Alicja Winczura, Z. Kazimierczuk, D. Gasparutto, T. Carell, B. Tudek, B. Castaing   +12 more
semanticscholar   +1 more source

Structure of Formamidopyrimidine-DNA Glycosylase Covalently Complexed to DNA [PDF]

Journal of Biological Chemistry, 2002
Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme that excises oxidized purines from damaged DNA. The Schiff base intermediate formed during this reaction between Escherichia coli Fpg and DNA was trapped by reduction with sodium borohydride, and the structure of the resulting covalently cross-linked complex was determined at a 2.1-A ...
Rotem, Gilboa, Dmitry O, Zharkov, Gali, Golan, Andrea S, Fernandes, Sue Ellen, Gerchman, Eileen, Matz, Jadwiga H, Kycia, Arthur P, Grollman, Gil, Shoham   +8 more
openaire   +2 more sources

Pulmonary Vascular Endothelial Cells in Lung Diseases: Mechanisms, Therapeutic Strategies, and Future Directions

Cell Proliferation, EarlyView.
The pathogenic mechanisms of pulmonary vascular endothelial cells (VECs) in lung diseases and their multimodal therapeutic strategies. ABSTRACT Pulmonary vascular endothelial cells (VECs) are essential for the normal function of the lung, through maintaining vascular barrier integrity, regulating blood flow, and participating in inflammatory responses ...
Qianyue Liu, Hongshuai Zheng, Jing Liu, Ming Gao, Faquan Lin, Lin Liao   +5 more
wiley   +1 more source

Uracil-DNA Glycosylase UNG Promotes Tet-mediated DNA Demethylation [PDF]

Journal of Biological Chemistry, 2016
In mammals, active DNA demethylation involves oxidation of 5-methylcytosine (5mC) into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by Tet dioxygenases and excision of these two oxidized bases by thymine DNA glycosylase (TDG). Although TDG is essential for active demethylation in embryonic stem cells and induced pluripotent stem cells, it is ...
Jian-Huang, Xue, Gui-Fang, Xu, Tian-Peng, Gu, Guo-Dong, Chen, Bin-Bin, Han, Zhi-Mei, Xu, Magnar, Bjørås, Hans E, Krokan, Guo-Liang, Xu, Ya-Rui, Du   +9 more
openaire   +2 more sources

Genome‐wide and gene‐specific DNA methylation across developmental stages in Pogonomyrmex californicus: A socially polymorphic ant

Insect Molecular Biology, EarlyView.
Comparison between developmental stages (larvae, pupae, worker) in Pogonomyrmex californicus revealed significant stage‐specific differences in Gene Body Methylated frequencies. Methylation sites were highly correlated between WGBS and ONT in P. californicus Genome‐wide methylation was low (~3%) and highly clustered within gene bodies (GBM), especially
Tania Chavarria‐Pizarro, Mohammed Errbii, Janina Rinke, Lukas Schrader, Jürgen Gadau   +4 more
wiley   +1 more source

Mechanism of Werner DNA helicase: POT1 and RPA stimulates WRN to unwind beyond gaps in the translocating strand. [PDF]

PLoS ONE, 2009
WRN belongs to the RecQ family of DNA helicases and it plays a role in recombination, replication, telomere maintenance and long-patch base excision repair. Here, we demonstrate that WRN efficiently unwinds DNA substrates containing a 1-nucleotide gap in
Byungchan Ahn, Jae Wan Lee, Hana Jung, Gad Beck, Vilhelm A Bohr   +4 more
doaj   +1 more source

Supplemental Figure Legends from ATM Regulates 3-Methylpurine-DNA Glycosylase and Promotes Therapeutic Resistance to Alkylating Agents [PDF]

, 2023
Sameer Agnihotri, Kelly Burrell, Pawel Buczkowicz, Marc Remke, Brian Golbourn, Yevgen Chornenkyy, Aaron S. Gajadhar, Nestor A. Fernandez, Ian D. Clarke, Mark S. Barszczyk, Sanja Pajovic, Christian Ternamian, Renee Head, N. Sabha, Robert W. Sobol, Michael D. Taylor, James T. Rutka, Chris Jones, Peter B. Dirks, Gelareh Zadeh, Cynthia Hawkins   +20 more
openalex   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, EarlyView.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

Thymine DNA glycosylase specifically recognizes 5-carboxylcytosine-modified DNA [PDF]

Nature Chemical Biology, 2012
Human thymine DNA glycosylase (hTDG) efficiently excises 5-carboxylcytosine (5caC), a key oxidation product of 5-methylcytosine in genomic DNA, in a recently discovered cytosine demethylation pathway. We present here the crystal structures of the hTDG catalytic domain in complex with duplex DNA containing either 5caC or a fluorinated analog.
Zhang, Liang, Lu, Xingyu, Lu, Junyan, Liang, Haihua, Dai, Qing, Xu, Guo-Liang, Luo, Cheng, Jiang, Hualiang, He, Chuan   +8 more
openaire   +2 more sources

Bacillus subtilis RNase HII Is Inefficient at Processing Guanosine Monophosphate and Damaged Ribonucleotides

Molecular Microbiology, EarlyView.
Bacillus subtilis RNase HII corrects ribonucleotide errors and mismatched ribonucleotides, but not damaged ribonucleotides. Mismatched ribonucleotides would also serve as substrates for mismatch repair while damaged ribonucleotides would be addressed through base excision repair.
Julianna R. Cresti, Lyle A. Simmons
wiley   +1 more source