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Electrophoretic Analysis of the DNA Supercoiling Activity of DNA Gyrase
2018Most bacterial cells have a motor enzyme termed DNA gyrase, which is a type-2 topoisomerase that reduces the linking number (Lk) of DNA. The supercoiling energy generated by gyrase is essential to maintain the bacterial chromosome architecture and regulate its DNA transactions. This chapter describes the use of agarose-gel electrophoresis to detect the
Belén, Martínez-García +5 more
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DNA Supercoiling Catalyzed by Bacterial Gyrase
DNA gyrase is an essential type II topoisomerase that is conserved across bacteria species and has an essential function of resolving overwound (positive supercoiled) DNA and introducing negative supercoils into relaxed DNA. The overall catalytic activity of gyrase can be determined using in vitro assays utilizing purified enzyme subunits and a DNA ...Samika, Joshi, Neil, Osheroff
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Recent advances in DNA gyrase-targeted antimicrobial agents
European Journal of Medicinal Chemistry, 2020Satish N Dighe, Trudi Collet
exaly
Exploiting bacterial DNA gyrase as a drug target: current state and perspectives
Applied Microbiology and Biotechnology, 2011Frédéric Collin +2 more
exaly