Results 21 to 30 of about 38,912 (254)

DNA supercoiling in gyrase mutants [PDF]

Journal of Bacteriology, 1984
Nucleoids isolated from Escherichia coli strains carrying temperature-sensitive gyrA or gyrB mutations were examined by sedimentation in ethidium bromide-containing sucrose density gradients. A shift to restrictive temperature resulted in nucleoid DNA relaxation in all of the mutant strains.
T R, Steck, G J, Pruss, S H, Manes, L, Burg, K, Drlica   +4 more
openaire   +2 more sources

Structural insights into the quinolone resistance mechanism of Mycobacterium tuberculosis DNA gyrase. [PDF]

PLoS ONE, 2010
Mycobacterium tuberculosis DNA gyrase, an indispensable nanomachine involved in the regulation of DNA topology, is the only type II topoisomerase present in this organism and is hence the sole target for quinolone action, a crucial drug active against ...
Jérémie Piton, Stéphanie Petrella, Marc Delarue, Gwénaëlle André-Leroux, Vincent Jarlier, Alexandra Aubry, Claudine Mayer   +6 more
doaj   +1 more source

Crystallization and preliminary crystallographic analysis of the DNA gyrase B protein from B-stearothermophilus [PDF]

, 1996
DNA gyrase B (GyrB) from B. stearothermophilus has been crystallized in the presence of the non-hydrolyzable ATP analogue, 5'-adenylpl-beta-gamma-imidodiphosphate (ADPNP), by the dialysis method.
Brannigan, J A, Subramanya, H S, Tsai, F T F, Wigley, D B, Wilkinson, A J   +4 more
core   +2 more sources

CryoEM structures of open dimers of gyrase A in complex with DNA illuminate mechanism of strand passage

eLife, 2018
Gyrase is a unique type IIA topoisomerase that uses ATP hydrolysis to maintain the negatively supercoiled state of bacterial DNA. In order to perform its function, gyrase undergoes a sequence of conformational changes that consist of concerted gate ...
Katarzyna M Soczek, Tim Grant, Peter B Rosenthal, Alfonso Mondragón   +3 more
doaj   +1 more source

Design and synthesis of new 3-((7-chloroquinolin-4-yl)amino)thiazolidin-4-one analogs as Mycobacterium tuberculosis DNA gyrase inhibitors

Future Journal of Pharmaceutical Sciences, 2021
Background Tuberculosis is evidently a major health threat among human populations worldwide. The current study presents the synthesis of new 3-((7-chloroquinolin-4-yl)amino)thiazolidin-4-one analogs (4a–o) as potential Mycobacterium tuberculosis DNA ...
Preeti S. Salve, Prajakta Parchure, Lisel Araujo, Rohini S. Kavalapure, Sunil S. Jalalpure, Dharmarajan Sriram, Vagolu Siva Krishna, Madhu Rekha Estharla, Shankar G. Alegaon   +8 more
doaj   +1 more source

A Crystal Structure of the Bifunctional Antibiotic Simocyclinone D8, Bound to DNA Gyrase [PDF]

, 2009
Simocyclinones are bifunctional antibiotics that inhibit bacterial DNA gyrase by preventing DNA binding to the enzyme. We report the crystal structure of the complex formed between the N-terminal domain of the Escherichia coli gyrase A subunit and ...
A. Maxwell, A. R. McKay, C. E.M. Stevenson, D. M. Lawson, Flatman, Flatman, Galm, H.-P. Fiedler, L. A. Mitchenall, Laponogov, Lewis, Li, Livermore, M. J. Buttner, M. J. Edwards, Maxwell, Maxwell, Morais Cabral, Oliphant, R. H. Flatman, Ruiz, Schimana, Schoeffler, T. A. Clarke, T. B.K. Le, Theobald, Trefzer, Tsai, Yoshida, Yoshida   +29 more
core   +1 more source

PprA Protein Is Involved in Chromosome Segregation via Its Physical and Functional Interaction with DNA Gyrase in Irradiated Deinococcus radiodurans Bacteria

mSphere, 2016
PprA, a radiation-induced Deinococcus-specific protein, was previously shown to be required for cell survival and accurate chromosome segregation after exposure to ionizing radiation. Here, we used an in vivo approach to determine, by shotgun proteomics,
Alice Devigne, Philippe Guérin, Johnny Lisboa, Sophie Quevillon-Cheruel, Jean Armengaud, Suzanne Sommer, Claire Bouthier de la Tour, Pascale Servant   +7 more
doaj   +1 more source

Complexation of norfloxacin with DNA in the presence of caffeine [PDF]

, 2006
1H NMR spectroscopy (500 MHz) has been used to quantify the complexation of the antibacterial antibiotic Norfloxacin (NOR) with DNA in the presence of Caffeine (CAF). Separate studies have been made for the self-association of NOR, its hetero-association
Davies, David B., Evstigneev, M.P., Rybakova, K.A.   +2 more
core   +1 more source

Regulation of DNA supercoiling in Escherichia coli: Genetic basis of a compensatory mutation in DNA gyrase [PDF]

, 1989
Bacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a topA deletion mutant of Escherichia coli (DM800) that has a ...
Wang, Pruss, DiNardo, Gellert, Pritchard, Mizuuchi, Maxam, Sanger, Adachi, Brown, Mizuuchi, Gellert, Price, Fisher   +13 more
core   +5 more sources

Mechanism of inhibition of DNA gyrase by cyclothialidine, a novel DNA gyrase inhibitor [PDF]

Antimicrobial Agents and Chemotherapy, 1994
We investigated how cyclothialidine (Ro 09-1437), a novel DNA gyrase inhibitor belonging to a new chemical class of compounds, acts to inhibit Escherichia coli DNA gyrase. Cyclothialidine up to 100 micrograms/ml showed no effect on DNA gyrase when linear DNA was used as a substrate.
N, Nakada, H, Gmünder, T, Hirata, M, Arisawa   +3 more
openaire   +2 more sources