Results 231 to 240 of about 533,967 (266)
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Integrated capillary fluorescence DNA biosensor
Biosensors and Bioelectronics, 2003Covalent attachment of dsDNA molecules inside a glass capillary without the need for hybridization is described. It is shown that the glass capillary has a surface density of 2.5 x 10(13) molecules/cm(2) with specific binding capacity of 62.5%. The resulting substrate was used to develop a biosensor for determining fluorescent organic analytes and ...
Marc A, Breimer +2 more
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Gamete Research, 1979
AbstractEjaculated rabbit spermatozoa washed with buffer prior to decondensation by Triton X‐100 and dithiothreitol were good templates for DNA synthesis by Escherichia coli DNA polymerase. This result agrees with the observations of Zirkin and Chang [1977], and implies that the sperm DNA is nicked.
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AbstractEjaculated rabbit spermatozoa washed with buffer prior to decondensation by Triton X‐100 and dithiothreitol were good templates for DNA synthesis by Escherichia coli DNA polymerase. This result agrees with the observations of Zirkin and Chang [1977], and implies that the sperm DNA is nicked.
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Jumping at the chance for precise DNA integration
Nature Biotechnology, 2019CRISPR–Cas-associated transposases enable targeted integration of new sequences into genomes.
Jennifer B. Kwon, Charles A. Gersbach
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Integrated Microfluidic Systems for DNA Analysis
2011The potential utility of genome-related research in terms of evolving basic discoveries in biology has generated widespread use of DNA diagnostics and DNA forensics and driven the accelerated development of fully integrated microfluidic systems for genome processing.
Samuel K, Njoroge +3 more
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Sperm DNA integrity and male infertility
Urology, 2005i r ammalian fertilization involves the direct interaction of the sperm and oocyte, fusion of he cell membranes, and union of male and female amete genomes.1 The completion of this process nd subsequent embryo development depend in art on the inherent integrity of the sperm DNA.2 A hreshold of sperm DNA damage (i.e., DNA fragentation, abnormal ...
Jeanne, O'Brien, Armand, Zini
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Integrating gold nanostars into condensed DNA
Biochimica et Biophysica Acta (BBA) - General SubjectsX-irradiation has extensive applications in therapy and considerable attention has been devoted to the radiosensitizing properties of nanoparticles composed of high atomic number elements, particularly gold. Low energy electrons and/or heterogenous catalysis are widely suspected to be involved in radiosensitization, but there is uncertainty about their
Christopher C, Perry +6 more
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Integrating DNA: Transposases and Retroviral Integrases
Annual Review of Microbiology, 1999▪ Abstract Transposable elements appear quite disparate in their organization and in the types of genetic rearrangements they promote. In spite of this diversity, retroviruses and many transposons of both prokaryotes and eukaryotes show clear similarities in the chemical reactions involved in their transposition.
L, Haren, B, Ton-Hoang, M, Chandler
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Transforming DNA integrates into the host chromosome
Cell, 1981A series of rat liver cotransformed cell lines have been constructed containing from 5 to 100 copies of a variant human growth hormone gene. We have used hybridization in situ to demonstrate that most, if not all, cotransformed sequences reside in a chromosome of the host cell.
D M, Robins +3 more
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Integration of DNA Tumor Virus Genomes
1976It has been known for some time that certain bacteriophage genomes, such as lambda, can be covalently integrated into the host bacterial chromosome. Of particular interest is the observation that this integration leads to lysogeny, a condition which affects the fate of both the phage and the host bacterium.
M A, Martin, G, Khoury
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Deoxyribonucleotides and Disorders of Mitochondrial DNA Integrity
DNA and Cell Biology, 2004Mitochondrial DNA (mtDNA) depends on numerous nuclear encoded factors and a constant supply of deoxyribonucleoside triphosphates (dNTP), for its maintenance and replication. The function of proteins involved in nucleotide metabolism is perturbed in a heterogeneous group of disorders associated with depletion, multiple deletions, and mutations of the ...
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