Results 61 to 70 of about 1,362,179 (211)

DNA isolation method is a source of global DNA methylation variability measured with LUMA. Experimental analysis and a systematic review.

PLoS ONE, 2013
In DNA methylation, methyl groups are covalently bound to CpG dinucleotides. However, the assumption that methyl groups are not lost during routine DNA extraction has not been empirically tested.
Carolina Soriano-Tárraga, Jordi Jiménez-Conde, Eva Giralt-Steinhauer, Angel Ois, Ana Rodríguez-Campello, Elisa Cuadrado-Godia, Israel Fernández-Cadenas, Joan Montaner, Gavin Lucas, Roberto Elosua, Jaume Roquer, GeneStroke “The Spanish Stroke Genetics Consortium”   +11 more
doaj   +1 more source

Isolation and Quantification of Extracellular DNA from Biofluids

Bio-Protocol, 2020
Extracellular DNA is studied as a diagnostic biomarker, but also as a factor involved in the pathophysiology of several diseases due to its pro-inflammatory properties.
Ľubica Janovičová, Barbora Konečná, Barbora Vlková, Peter Celec   +3 more
doaj   +1 more source

Prochloron research [PDF]

The purpose was to prepare Prochloron photosynthetic membranes for the isolation of the two major chlorophyll-proteins, the P700-chlorophyll a-protein and the light-harvesting chlorophyll a/b-protein, using SDS-polyacrylamide gel electrophoresis.
Cheng, L., Lewin, R. A.
core   +1 more source

BacS: An Abundant Bacteroid Protein in \u3cem\u3eRhizobium etli\u3c/em\u3e Whose Expression Ex Planta Requires \u3cem\u3enifA\u3c/em\u3e [PDF]

, 2003
Rhizobium etli CFN42 bacteroids from bean nodules possessed an abundant 16-kDa protein (BacS) that was found in the membrane pellet after cell disruption. This protein was not detected in bacteria cultured in tryptone-yeast extract.
Davila, Guillermo, Jahn, Olivia Jeanne, Noel, K. Dale, Romero, David   +3 more
core   +1 more source

Plasmide DNA Isolation from Bacteria and Transfection to HEK293 Cell Line [PDF]

, 2015
Isolation DNA is a one of the basic methods in molecular biology. There are several methods of DNA amplification and isolation. In this paper phenol-chloroform extraction of three plasmid types is used: Channelrhodopsin-2, ASAP and KIR.
Karmazínová, K.
core  

Transformation of the Methylotrophic Actinomycete Amycolatopis methanolica with Plasmid DNA: Stimulatory Effect of a pMEA300-Encoded Gene [PDF]

, 1995
Amycolatopsis methanolica contains a 13.29-kb plasmid (pMEA300) present both in the free state and integrated at a unique genomic location. A pMEA300-free derivative (strain WV1) was selected, allowing further analysis of pMEA300-encoded functions. Whole
Dijkhuizen, L.,, Madoń, J.,, Vrijbloed, J.W.,   +2 more
core   +2 more sources

Tomato: a crop species amenable to improvement by cellular and molecular methods [PDF]

, 1989
Tomato is a crop plant with a relatively small DNA content per haploid genome and a well developed genetics. Plant regeneration from explants and protoplasts is feasable which led to the development of efficient transformation procedures.
A. Crossway, A. Guri, A. Hoekema, A. Kinshara, A. Morgan, A. Zelcer, A.H. Paterson, A.J. Framond De, A.L. Phillips, A.L. Phillips, A.R. Krol Van der, B. Baker, B. Baker, B. Hause, B. McClintock, B. Piechulla, B.A. Levenko, B.D. Harrison, B.L. Ward, B.P. Moens, B.R. Thomas, B.R. Thomas, B.S. Gill, B.S. Landry, B.S. Landry, B.S. Landry, C. Chang, C. Pieterse, C. Waldron, C.D. Riggs, C.E. Vallejos, C.E. Vallejos, C.J.S. Smith, C.M. Rick, C.M. Rick, C.M. Rick, C.M. Rick, C.M. Rick, C.M. Rick, C.M. Rick, C.M. Rick, C.M. Smith, C.P. Meredith, D. Boer De, D. Botstein, D. Shah, D. Zamir, D. Zamir, D. Zamir, D.A. Evans, D.A. Evans, D.M. Barker, D.M. Lonsdale, D.P. Barlow, D.W. Barton, D.W. Galbraith, E. Haute Van, E. Pichersky, E.A. Frankenberger, E.A. Frankenberger, E.A. Shahin, E.A. Shahin, E.C. Tigchelaar, F.J. Zapata, G. Gavazzi, G. Melchers, G. Schweizer, G.S. Khush, G.S. Khush, H.P. Mühlbach, I. Negrutiu, I.M. Greenblat, J. Botterman, J. Haseloff, J. Hille, J. Hille, J. Paszkowski, J. Phillips, J. Wijbrandi, J.D. Palmer, J.D. Palmer, J.D. Palmer, J.D. Palmer, J.F. Gusella, J.F. Gusella, J.F. Shepard, J.G. Atherton, J.H. Hawkes, J.I. Yoder, J.J. Fillatti, J.L. Bennetzen, J.M. van Montagu Schell, J.S. Beckmann, J.S. Beckmann, J.W. Maxon-Smith, J.W. Verna De, Jacoues Hille, K. Ohyama, K. Shinozaki, K. Umesono, K.A. Darden, K.K. Kartha, L. Herrera-Estrella, L. Herrera-Estrella, L. Marton, L.A. Miki, L.P. Hosticka, L.W. Handley, M. Block De, M. Buiatti, M. Buiatti, M. Cuozzo, M. Jongsma, M. Koornneef, M. Koornneef, M. Mutschler, M. S. Ramanna, M. Sibi, M. Soller, M. Vaeek, M. Wallroth, M. Ziv, M.A. O'Connell, M.A. O'Connell, M.A. O'Connell, M.A. O'Connell, M.A. Sluys Van, M.A. Stevens, M.A. Stevens, M.D. Bennett, M.L.M.C. Tan, M.L.M.C. Tan, M.R. Hanson, M.S. Ramanna, M.S. Ramanna, M.S. Ramanna, M.W. Bevan, M.W. Ganal, M.Y. Menzel, Maarten Koornneef, N.A. Zagorska, N.D. Young, N.D. Young, N.E. Tumer, N.V. Federoff, P. Little, P. Zambryski, P.E. McClean, P.F. Ambros, P.G. Smith, P.J.M. Elzen Van den, P.J.M. Elzen Van den, P.M. Gresshoff, Pim Zabel, R. Bernatzky, R. Bernatzky, R. Ecochard, R. Hain, R. Niedz, R.A. Bressan, R.A.J. Daelen Van, R.B. Flavell, R.B. Horsch, R.D. Locky, R.D. Shillito, R.S. Nelson, R.T. Fraley, S. Dellaporta, S. McCormick, S. Ohki, S.D. Tanksley, S.D. Tanksley, S.D. Tanksley, S.D. Tanksley, S.D. Tanksley, S.D. Tanksley, St.G. Rogers, T. Helentjaris, T. Helentjaris, T. Helentjaris, T.C. Osborn, T.L. Adams, T.M. Klein, U. Wienand, V. Padmanabhan, V.A. Hilder, W. Gottschalk, W. Gruissem, W.L. Gerlach, W.R. Sharp, Y.S. Chyi, Z. Koukolikova-Nicola, Z. Tabaeizadeh   +192 more
core   +3 more sources

Circular DNA in Isolated Chromoplasts

Zeitschrift für Naturforschung C, 1974
Abstract Flower chromoplasts of Narcissus pseudonarcissus have been isolated in high purity and inte­ grity by a new procedure. From these chromoplasts circular DNA molecules in supercoiled form have been isolated and demonstrated by electron microscopy to have a contour length of about 44 μm (molecular weight about 92 x 106 daltons)
H, Falk, B, Liedvogel, P, Sitte
openaire   +2 more sources

Comparison of Different DNA Isolation Methods from Grapevine (Vitis vinifera) Leaves

Separations
The extraction of high-quality DNA is essential for molecular analyses in grapevine, yet differences among commonly used protocols remain underexplored.
Nina Buljević, Darko Preiner, Iva Šikuten, Ivana Tomaz   +3 more
doaj   +1 more source

Rapid Isolation of PCR-Ready DNA from Blood, Bone Marrow and Cultured Cells, Based on Paramagnetic Beads

BioTechniques, 1997
PCR-based methods for the analysis of genomic DNA are becoming increasingly common both in research and for routine purposes. A rapid, small-scale DNA isolation method is needed to take full advantage of the speed and automation potential of the PCR ...
Arne Deggerdal, Frank Larsen
doaj   +1 more source