Bacteriophage origin of some minimal ATP-dependent DNA ligases: a new structure from Burkholderia pseudomallei with striking similarity to Chlorella virus ligase [PDF]
, 2021 DNA ligases, the enzymes responsible for joining breaks in the phosphodiester backbone of DNA during replication and repair, vary considerably in size and structure.
Leiros, Hanna-Kirsti Schrøder +4 more
core +2 more sources
Making ends meet: repairing breaks in bacterial DNA by non-homologous end-joining. [PDF]
, 2006 DNA double-strand breaks (DSBs) are one of the most dangerous forms of DNA lesion that can result in genomic instability and cell death. Therefore cells have developed elaborate DSB-repair pathways to maintain the integrity of genomic DNA.
Bowater, Richard, Doherty, Aidan J
core +10 more sources
Evaluation of NAD(+)-dependent DNA ligase of mycobacteria as a potential target for antibiotics [PDF]
, 2007 Mycobacteria contain genes for several DNA ligases, including ligA, which encodes a NAD+-dependent enzyme that has been postulated to be a target for novel antibacterial compounds.
Bowater, Richard P. +6 more
core +2 more sources
Identification of a novel motif in DNA ligases exemplified by DNA ligase IV [PDF]
, 2006 DNA ligase IV is an essential protein that functions in DNA non-homologous end-joining, the major mechanism that rejoins DNA double-strand breaks in mammalian cells. LIG4 syndrome represents a human disorder caused by mutations in DNA ligase IV that lead
Doherty, Aidan J +5 more
core +1 more source
NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor [PDF]
, 2003 Sequencing of the genomes of Mycobacterium tuberculosis H37Rv and Streptomyces coelicolor A3(2) identified putative genes for an NAD+-dependent DNA ligase. We have cloned both open reading frames and overexpressed the protein products in Escherichia coli.
Bowater, Richard +6 more
core +1 more source
Label-free electrochemical monitoring of DNA ligase activity [PDF]
, 2008 This study presents a simple, label-free electrochemical technique for the monitoring of DNA ligase activity. DNA ligases are enzymes that catalyze joining of breaks in the backbone of DNA and are of significant scientific interest due to their essential
Ahel I. +29 more
core +1 more source
Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4 [PDF]
, 2006 The genome of bacteriophage T4 encodes three polynucleotide ligases, which seal the backbone of nucleic acids during infection of host bacteria. The T4Dnl (T4 DNA ligase) and two RNA ligases [T4Rnl1 (T4 RNA ligase 1) and T4Rnl2] join a diverse array of ...
Amitsur +61 more
core +4 more sources
Characterization of a temperature-sensitive DNA ligase from Escherichia coli [PDF]
, 2004 DNA ligases are essential enzymes in cells due to their ability to join DNA strand breaks formed during DNA replication. Several temperature-sensitive mutant strains of Escherichia coli, including strain GR501, have been described which can be ...
Bowater, Laura +8 more
core +1 more source
Analysis of ligation and DNA binding by Escherichia coli DNA ligase (LigA). [PDF]
, 2005 NAD+-dependent DNA ligases are essential enzymes in bacteria, with the most widely studied of this class of enzymes being LigA from Escherichia coli. NAD+-dependent DNA ligases comprise several discrete structural domains, including a BRCT domain at the ...
Adam Wilkinson +59 more
core +1 more source
Structural basis for the RING catalyzed synthesis of K63 linked ubiquitin chains [PDF]
, 2015 This work was supported by grants from Cancer Research UK (C434/A13067), the Wellcome Trust (098391/Z/12/Z) and Biotechnology and Biological Sciences Research Council (BB/J016004/1).The RING E3 ligase catalysed formation of lysine 63 linked ubiquitin ...
A Plechanovová +42 more
core +3 more sources