Bacteriophage origin of some minimal ATP-dependent DNA ligases: a new structure from Burkholderia pseudomallei with striking similarity to Chlorella virus ligase [PDF]
Scientific Reports, 2021 DNA ligases, the enzymes responsible for joining breaks in the phosphodiester backbone of DNA during replication and repair, vary considerably in size and structure.
Jolyn Pan +4 more
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A role for the ATP-dependent DNA ligase lig E of Neisseria gonorrhoeae in biofilm formation [PDF]
BMC MicrobiologyBackground The ATP-dependent DNA ligase Lig E is present as an accessory DNA ligase in numerous proteobacterial genomes, including many disease-causing species.
Jolyn Pan +4 more
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Crystal structure of ATP-dependent DNA ligase from Rhizobium phage vB_RleM_P10VF. [PDF]
Acta Crystallogr F Struct Biol CommunDNA ligases are foundational molecular-biological tools used for cloning and sequencing workflows, and are essential replicative enzymes for all cellular life forms as well as many viruses and bacteriophage. There is considerable interest in structurally and functionally characterizing novel DNA ligases and profiling their suitability for molecular ...
Rothweiler U, Leiros HKS, Williamson A.
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Crystal Structure of an ATP-Dependent DNA Ligase from Bacteriophage T7 [PDF]
Cell, 1996 The crystal structure of the ATP-dependent DNA ligase from bacteriophage T7 has been solved at 2.6 A resolution. The protein comprises two domains with a deep cleft running between them. The structure of a complex with ATP reveals that the nucleotide binding pocket is situated on the larger N-terminal domain, at the base of the cleft between the two ...
Hosahalli S. Subramanya +3 more
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Characterization of an ATP-dependent DNA ligase from the thermophilic archaeon Methanobacterium thermoautotrophicum [PDF]
Nucleic Acids Research, 2000 We report the production, purification and characterization of a DNA ligase encoded by the thermophilic archaeon Methanobacterium thermoautotrophicum. The 561 amino acid MTH: ligase catalyzed strand-joining on a singly nicked DNA in the presence of a divalent cation (magnesium, manganese or cobalt) and ATP (K(m) 1.1 microM). dATP can substitute for ATP,
Verl Sriskanda
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ATP-dependent DNA ligases. [PDF]
Genome biology, 2002 By catalyzing the joining of breaks in the phosphodiester backbone of duplex DNA, DNA ligases play a vital role in the diverse processes of DNA replication, recombination and repair. Three related classes of ATP-dependent DNA ligase are readily apparent in eukaryotic cells.
Ina V. Martin, Stuart A. MacNeill
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Characterization of a Baculovirus-Encoded ATP-Dependent DNA Ligase [PDF]
Journal of Virology, 1998 ABSTRACTSequence analysis of theLymantria disparmulticapsid nucleopolyhedrovirus (LdMNPV) genome identified an open reading frame (ORF) encoding a 548-amino-acid (62-kDa) protein that showed 35% amino acid sequence identity with vaccinia virus ATP-dependent DNA ligase. Ligase homologs have not been reported from other baculoviruses.
Margot Pearson, George F Rohrmann
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A role for the ATP-dependent DNA ligase Lig E of Neisseria gonorrhoeae in biofilm formation [PDF]
, 2023 Abstract The ATP-dependent DNA ligase Lig E is present as an accessory DNA ligase in numerous proteobacterial genomes, including many disease-causing species. Here we have constructed a genomic Lig E knock-out in the obligate human pathogen Neisseria gonorrhoeae and characterised its growth and infection characteristics.
Jolyn Pan +2 more
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Enzyme–adenylate structure of a bacterial ATP-dependent DNA ligase with a minimized DNA-binding surface [PDF]
Acta Crystallographica Section D Biological Crystallography, 2014 DNA ligases are a structurally diverse class of enzymes which share a common catalytic core and seal breaks in the phosphodiester backbone of double-stranded DNAviaan adenylated intermediate. Here, the structure and activity of a recombinantly produced ATP-dependent DNA ligase from the bacteriumPsychromonassp. strain SP041 is described.
Adele Williamson +2 more
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T4 DNA ligase structure reveals a prototypical ATP-dependent ligase with a unique mode of sliding clamp interaction [PDF]
Nucleic Acids Research, 2018 DNA ligases play essential roles in DNA replication and repair. Bacteriophage T4 DNA ligase is the first ATP-dependent ligase enzyme to be discovered and is widely used in molecular biology, but its structure remained unknown. Our crystal structure of T4 DNA ligase bound to DNA shows a compact α-helical DNA-binding domain (DBD), nucleotidyl-transferase
Ke Shi +11 more
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