Discovery and In Vitro Reconstitution of Closoxazole Biosynthesis from Pyxidicoccus fallax. [PDF]
ChembiochemLernoud L +4 more
europepmc +1 more source
Mutation- and Transcription-Driven Omic Burden of Daptomycin/Dalbavancin-R and Glycopeptide-RS Fitness Costs in High-Risk MRSA: A Nexus in Antimicrobial Resistance Mechanisms-Genome Proneness-Compensatory Adaptations. [PDF]
Antibiotics (Basel)Chines E +4 more
europepmc +1 more source
High fat diet (HFD) induced hepatic lipogenic metabolism and lipotoxicity via Parkin-dependent mitophagy and Errα signal of Pelteobagrus fulvidraco. [PDF]
J Anim Sci BiotechnolYu A +6 more
europepmc +1 more source
Biochemical characterization of an ATP-dependent DNA ligase from the hyperthermophilic crenarchaeon Sulfolobus shibatae [PDF]
Extremophiles, 2002 A gene encoding a putative ATP-dependent DNA ligase was identified in the genome of the hyperthermophilic archaeon Sulfolobus shibatae and expressed in Escherichia coli. The 601 amino acid recombinant polypeptide was a monomeric protein capable of strand joining on a singly nicked DNA substrate in the presence of ATP ( K(m)=34 micro mu) and a divalent ...
Hongbing Shao +3 more
semanticscholar +6 more sources
Free-labelled fluorescent method for ATP detection assisted by T4 DNA ligase
Analytical Methods, 2017 A facile, highly selective biosensor was used for ATP quantitative analysis.
Hongyun Huang +5 more
semanticscholar +4 more sources
The α-thio and/or β-γ-hypophosphate analogs of ATP as cofactors of T4 DNA ligase
Bioorganic Chemistry, 2016 T4 DNA ligase is one of the most commonly used enzymes for in vitro molecular research and a useful model for testing the ligation mechanism of ATP-dependent DNA ligation. To better understand the influence of phosphate group modifications in the ligation process, a series of ATP analogs were tested as cofactors.
Roza Pawlowska +4 more
semanticscholar +5 more sources
Characterization of an ATP-dependent type I DNA ligase from Arabidopsis thaliana. [PDF]
Plant molecular biology, 2001 Here we report the purification and biochemical characterization of recombinant Arabidopsis thaliana DNA ligase I. We show that this ligase requires ATP as a source for adenylation. The calculated Km [ATP] for ligation is 3 microM. This enzyme is able to ligate nicks in oligo(dT)/poly(dA) and oligo(rA)/poly(dT) substrates, but not in oligo(dT)/poly(rA)
Alicja Ziemienowicz +2 more
semanticscholar +4 more sources
Mechanistic and Kinetic Study of the ATP-Dependent DNA Ligase of Neisseria meningitidis
Biochemistry, 2003 The gene from Neisseria meningitidis serogroup A, encoding a putative, secreted ATP-dependent DNA ligase was cloned and overexpressed, and the soluble protein was purified. Mass spectrometry indicated that the homogeneous protein was adenylated as isolated, and sedimentation velocity experiments suggested that the enzyme exists as a monomer in solution.
John S. Blanchard, Sophie Magnet
semanticscholar +5 more sources
International Journal of Biological Macromolecules, 2023 The genome sequence of hyperthermophilic archaeon Pyrobaculum calidifontis contains an open reading frame, Pcal_0039, which encodes a putative DNA ligase. Structural analysis disclosed the presence of signature sequences of ATP-dependent DNA ligases. We have heterologously expressed Pcal_0039 gene in Escherichia coli.
Qamar, Abbas +4 more
openaire +3 more sources