Results 21 to 30 of about 145,020 (273)
A Primer-dependent Polymerase Function of Pseudomonas aeruginosa ATP-dependent DNA Ligase (LigD) [PDF]
Journal of Biological Chemistry, 2004 Pseudomonas aeruginosa encodes two putative DNA ligases: a classical NAD(+)-dependent DNA ligase (LigA) plus an ATP-dependent DNA ligase (LigD). LigD exemplifies a family of bacterial proteins that consist of a ligase domain fused to flanking domains that resemble nucleases and/or polymerases. Here we purify LigD and show that it possesses an intrinsic
Hui Zhu, Stewart Shuman
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Characterization of an ATP-dependent DNA ligase encoded by Haemophilus influenzae [PDF]
Nucleic Acids Research, 1997 We report that Haemophilus influenzae encodes a 268 amino acid ATP-dependent DNA ligase. The specificity of Haemophilus DNA ligase was investigated using recombinant protein produced in Escherichia coli. The enzyme catalyzed efficient strand joining on a singly nicked DNA in the presence of magnesium and ATP (Km = 0.2 microM).
Chonghui Cheng
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Use of ATP, dATP and their α-thio derivatives to study DNA ligase adenylation [PDF]
Biochemical Journal, 1990 Bacteriophage-T4 and human type I DNA ligases were found capable of self-adenylating upon exposure to both ribo- and deoxyribo-[alpha-35S]thio-ATP. However, the joining reaction does not take place in the presence of the deoxyribotriphosphates. Enzyme adenylation is reversed in all cases by an excess of PPi, but the rate of reversion is lower with thio
Alessandra Montecucco +4 more
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Characterization of an ATP-dependent DNA ligase from the acidophilic archaeon “Ferroplasma acidarmanus” Fer1 [PDF]
Extremophiles, 2006 Analysis of the genome of "Ferroplasma acidarmanus" Fer1, an archaeon that is an extreme acidophile, identified an open reading frame encoding a putative ATP-dependent DNA ligase, which we termed FaLig. The deduced amino acid sequence of FaLig contains 595 amino acids, with a predicted molecular mass of 67.8 kDa. "F.
Brian R. Jackson +4 more
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The three-dimensional structure of an ATP-dependent DNA ligase from bacteriophage T7 [PDF]
Acta Crystallographica Section A Foundations of Crystallography, 1996 H. S. Subramanya +3 more
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Recombinant expression and purification of an ATP-dependent DNA ligase from Aliivibrio salmonicida [PDF]
Protein Expression and Purification, 2014 The genome of the psychrophilic fish-pathogen Aliivibrio salmonicida encodes a putative ATP-dependent DNA ligase in addition to a housekeeping NAD-dependent enzyme. In order to study the structure and activity of the ATP dependent ligase in vitro we have undertaken its recombinant production and purification from an Escherichia coli based expression ...
Adele Williamson, Hege Lynum Pedersen
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Characterization of a Thermophilic ATP-Dependent DNA Ligase from the Euryarchaeon Pyrococcus horikoshii [PDF]
Journal of Bacteriology, 2005 ABSTRACT Archaea encode a DNA ligase composed of a C-terminal catalytic domain typical of ATP-dependent ligases plus an N-terminal domain similar to that found in eukaryotic cellular and poxvirus DNA ligases. All archaeal DNA ligases characterized to date have ATP-dependent adenylyltransferase and nick-joining activities. However,
Niroshika Keppetipola, Stewart Shuman
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Biochemical characterization of an ATP-dependent DNA ligase from the hyperthermophilic crenarchaeon Sulfolobus shibatae [PDF]
Extremophiles, 2002 A gene encoding a putative ATP-dependent DNA ligase was identified in the genome of the hyperthermophilic archaeon Sulfolobus shibatae and expressed in Escherichia coli. The 601 amino acid recombinant polypeptide was a monomeric protein capable of strand joining on a singly nicked DNA substrate in the presence of ATP ( K(m)=34 micro mu) and a divalent ...
Xiaoqin Lai +3 more
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ATP-dependent DNA ligase fromArchaeoglobus fulgidusdisplays a tightly closed conformation [PDF]
Acta Crystallographica Section F Structural Biology and Crystallization Communications, 2009 DNA ligases join the breaks in double-stranded DNA by catalyzing the formation of a phosphodiester bond between adjacent 3'-hydroxyl and 5'-phosphate termini. They fall into two classes that require either ATP or NAD(+) as the source of an AMP group that is covalently attached to a strictly conserved lysine.
Dojin Kim +5 more
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BiofilmAbstractBackgroundNeisseria gonorrhoeae, the causative agent of the sexually transmitted infection, gonorrhoea, is known to form biofilms rich in extracellular DNA on human cervical cells. Biofilm formation is conducive to increased antimicrobial resistance and evasion of the host immune system, potentially causing asymptomatic infections.
Jolyn Pan +4 more
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