Biochemical Pharmacology, 2008 T4 DNA ligase and the ubiquitin activating enzyme (E1), catalyze the synthesis of ATP beta,gamma-bisphosphonate derivatives. Concerning T4 DNA ligase: (i) etidronate (pC(OH)(CH(3))p) displaced the AMP moiety of the complex E-AMP in a concentration dependent manner; (ii) the K(m) values and the rate of synthesis k(cat) (s(-1)), determined for the ...
Günther Sillero, María A. +3 more
semanticscholar +6 more sources
Identification of Novel Inhibitors of Escherichia coli DNA Ligase (LigA)
Molecules, 2021 Present in all organisms, DNA ligases catalyse the formation of a phosphodiester bond between a 3′ hydroxyl and a 5′ phosphate, a reaction that is essential for maintaining genome integrity during replication and repair.
Arqam Alomari +10 more
doaj +2 more sources
The three-dimensional structure of an ATP-dependent DNA ligase from bacteriophage T7 [PDF]
Acta Crystallographica Section A Foundations of Crystallography, 1996 A.J. Doherty +3 more
semanticscholar +4 more sources
ATP-dependent DNA ligases. [PDF]
Genome biology, 2002 By catalyzing the joining of breaks in the phosphodiester backbone of duplex DNA, DNA ligases play a vital role in the diverse processes of DNA replication, recombination and repair. Three related classes of ATP-dependent DNA ligase are readily apparent in eukaryotic cells.
Stuart A. MacNeill, Ina V. Martin
openaire +4 more sources
A role for the ATP-dependent DNA ligase Lig E of Neisseria gonorrhoeae in biofilm formation [PDF]
, 2023 Abstract The ATP-dependent DNA ligase Lig E is present as an accessory DNA ligase in numerous proteobacterial genomes, including many disease-causing species. Here we have constructed a genomic Lig E knock-out in the obligate human pathogen Neisseria gonorrhoeae and characterised its growth and infection characteristics.
Jolyn Pan +2 more
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Analysis of the distribution and evolution of the
Molecular Microbiology, 2015 SummaryPrior to the discovery of a minimal ATP‐dependent DNA ligase in Haemophilus influenzae, bacteria were thought to only possess a NAD‐dependent ligase, which was involved in sealing of Okazaki fragments. We now know that a diverse range of bacterial species possess up to six of these accessory bacterial ATP‐dependent DNA ligases (b‐ADLs), which ...
Adele Williamson +2 more
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Bleomycin-induced DNA repair by Saccharomyces cerevisiae ATP-dependent polydeoxyribonucleotide ligase [PDF]
Journal of Bacteriology, 1988 In contrast to ligase-deficient (cdc9) Saccharomyces cerevisiae, which did not rejoin bleomycin-induced DNA breaks, ligase-proficient (CDC9) yeast cells eliminated approximately 90% of DNA breaks within 90 to 120 min after treatment. Experimental conditions restricted enzymatic removal of the unusual 3'-phosphoglycolate termini in DNA cleaved by ...
C W Moore
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Synthesis of ATP derivatives of compounds of the mevalonate pathway (isopentenyl di- and triphosphate; geranyl di- and triphosphate, farnesyl di- and triphosphate, and dimethylallyl diphosphate) catalyzed by T4 RNA ligase, T4 DNA ligase and other ligases [PDF]
Biochemical Pharmacology, 2009 Compounds of the mevalonate pathway containing a terminal di- or triphosphate (mev-PP or mev-PPP) were tested as substrates of several enzyme ligases (T4 RNA ligase, T4 DNA ligase, firefly luciferase and other ligases) for the synthesis of ATP derivatives of the mev-pppA or mev-ppppA type.
Francisco J. Pérez-Zúñiga +5 more
openaire +6 more sources
ATP‐ and NAD+‐dependent DNA ligases share an essential function in the halophilic archaeon Haloferax volcanii [PDF]
Molecular Microbiology, 2005 SummaryDNA ligases join the ends of DNA molecules during replication, repair and recombination. ATP‐dependent ligases are found predominantly in the eukarya and archaea whereas NAD+‐dependent DNA ligases are found only in the eubacteria and in entomopoxviruses.
Fiona C. Gray +4 more
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In Bacteria, the replicative DNA ligase is NAD‐dependent. However, some bacteria possess accessory ATP‐dependent DNA ligases (ADLs) which vary in size and domain organization. Phylogenomic analyses of bacterial ADLs reveal that types Lig B, Lig C and Lig D have descended from a common ancestor, while Lig E was transferred in a separate event, possibly from a bacteriophage. For details, see the article by Williamson et al. on pp. 274‐290 of this issue. [PDF]
Molecular Microbiology, 2016 openaire +2 more sources