Characterization of a Thermophilic ATP-Dependent DNA Ligase from the Euryarchaeon Pyrococcus horikoshii [PDF]
Journal of Bacteriology, 2005 ABSTRACT Archaea encode a DNA ligase composed of a C-terminal catalytic domain typical of ATP-dependent ligases plus an N-terminal domain similar to that found in eukaryotic cellular and poxvirus DNA ligases. All archaeal DNA ligases characterized to date have ATP-dependent adenylyltransferase and nick-joining activities. However,
Niroshika Keppetipola, Stewart Shuman
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The three-dimensional structure of an ATP-dependent DNA ligase from bacteriophage T7 [PDF]
Acta Crystallographica Section A Foundations of Crystallography, 1996 H. S. Subramanya +3 more
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Biochemical Pharmacology, 2008 T4 DNA ligase and the ubiquitin activating enzyme (E1), catalyze the synthesis of ATP beta,gamma-bisphosphonate derivatives. Concerning T4 DNA ligase: (i) etidronate (pC(OH)(CH(3))p) displaced the AMP moiety of the complex E-AMP in a concentration dependent manner; (ii) the K(m) values and the rate of synthesis k(cat) (s(-1)), determined for the ...
Marı́a A. Günther Sillero +3 more
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Extremophiles, 2002 A gene encoding a putative ATP-dependent DNA ligase was identified in the genome of the hyperthermophilic archaeon Sulfolobus shibatae and expressed in Escherichia coli. The 601 amino acid recombinant polypeptide was a monomeric protein capable of strand joining on a singly nicked DNA substrate in the presence of ATP ( K(m)=34 micro mu) and a divalent ...
Lai, Shao Shao, Hao, Huang, Xq, Hb, Fy
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A role for the ATP-dependent DNA ligase Lig E of Neisseria gonorrhoeae in biofilm formation [PDF]
, 2023 Abstract The ATP-dependent DNA ligase Lig E is present as an accessory DNA ligase in numerous proteobacterial genomes, including many disease-causing species. Here we have constructed a genomic Lig E knock-out in the obligate human pathogen Neisseria gonorrhoeae and characterised its growth and infection characteristics.
Adele Williamson +2 more
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Bleomycin-induced DNA repair by Saccharomyces cerevisiae ATP-dependent polydeoxyribonucleotide ligase [PDF]
Journal of Bacteriology, 1988 In contrast to ligase-deficient (cdc9) Saccharomyces cerevisiae, which did not rejoin bleomycin-induced DNA breaks, ligase-proficient (CDC9) yeast cells eliminated approximately 90% of DNA breaks within 90 to 120 min after treatment. Experimental conditions restricted enzymatic removal of the unusual 3'-phosphoglycolate termini in DNA cleaved by ...
C W Moore
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ATP-dependent DNA ligases. [PDF]
Genome biology, 2002 By catalyzing the joining of breaks in the phosphodiester backbone of duplex DNA, DNA ligases play a vital role in the diverse processes of DNA replication, recombination and repair. Three related classes of ATP-dependent DNA ligase are readily apparent in eukaryotic cells.
Ina V. Martin, Stuart A. MacNeill
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Ligase IV, DNA, ATP-dependent (LIG4); BTB domain containing 2 (BTBD2); high mobility group AT-hook 2 (HMGA2); regulator of telomere elongation helicase 1 (RTEL1); coiled-coil domain containing 26 (CCDC26) [PDF]
Science-Business eXchange, 2010 openalex +3 more sources
The minimal Bacillus subtilis nonhomologous end joining repair machinery. [PDF]
PLoS ONE, 2013 It is widely accepted that repair of double-strand breaks in bacteria that either sporulate or that undergo extended periods of stationary phase relies not only on homologous recombination but also on a minimal nonhomologous end joining (NHEJ) system ...
Miguel de Vega
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The Transcriptional Response to DNA-Double-Strand Breaks in Physcomitrella patens. [PDF]
PLoS ONE, 2016 The model bryophyte Physcomitrella patens is unique among plants in supporting the generation of mutant alleles by facile homologous recombination-mediated gene targeting (GT).
Yasuko Kamisugi +2 more
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