Results 81 to 90 of about 29,739 (230)

CRISPR/Cas13a: Compensatory Target Activation Mechanism

Advanced Science, EarlyView.
CRISPR/Cas13a‐CTAM as a novel compensatory target activation mechanism that enables synergistic activation of Cas13a via two independently editable short RNA effectors. This dual‐effector system maintains enzymatic activity comparable to traditional single‐effector while significantly enhancing flexibility, sensitivity, and application scope.
Bowen Jiang, Tenghua Zhang, Yao Lu, Shujun Zhou, Wenjing Xiao, Shuming Pan, Nan Shi, Yan Sheng, Jiaming Hu   +8 more
wiley   +1 more source

Optimization of Enzymatic Logic Gates and Networks for Noise Reduction and Stability

, 2009
Biochemical computing attempts to process information with biomolecules and biological objects. In this work we review our results on analysis and optimization of single biochemical logic gates based on enzymatic reactions, and a network of three gates ...
Arugula, Mary A., Halamek, Jan, Katz, Evgeny, Melnikov, Dmitriy, Pita, Marcos, Privman, Vladimir, Strack, Guinevere   +6 more
core   +1 more source

QuASeR -- Quantum Accelerated De Novo DNA Sequence Reconstruction

, 2020
In this article, we present QuASeR, a reference-free DNA sequence reconstruction implementation via de novo assembly on both gate-based and quantum annealing platforms.
Al-Ars, Zaid, Bertels, Koen, Sarkar, Aritra   +2 more
core   +1 more source

Endogenous microRNA triggered enzyme-free DNA logic self-assembly for amplified bioimaging and enhanced gene therapy via in situ generation of siRNAs

Journal of Nanobiotechnology, 2021
Background Small interfering RNA (siRNA) has emerged as a kind of promising therapeutic agents for cancer therapy. However, the off-target effect and degradation are the main challenges for siRNAs delivery.
Qinghua Jiang, Shuzhen Yue, Kaixin Yu, Tian Tian, Jian Zhang, Huijun Chu, Zhumei Cui, Sai Bi   +7 more
doaj   +1 more source

TopCas: Topology‐Gated Cas12a via DNA‐RNA Chimeric Circular crRNA for Amplification‐Free Nucleic Acid Detection and Conditional Gene Editing

Advanced Science, EarlyView.
The TopCas platform utilizes an engineered DNA‐RNA chimeric circular crRNA to establish topology‐gated control over CRISPR‐Cas12a activity, enabling target‐triggered self‐catalytic amplification (preamplification‐free). This system seamlessly integrates highly sensitive molecular diagnostics with conditionally controlled gene editing, demonstrating ...
Shun Zhang, Wen Sun, Ting Xiao, Yali Wang, Xianlan Wu, Huiyou Chen, Ming Chen, Jun Zhang   +7 more
wiley   +1 more source

Supplementary. Logic gates based on DNA aptamers

, 2020
Supplementary.
Andrianova Mariia, Kuznetsov Alexander
openaire   +2 more sources

Optimization of SWCNT‐FET Biosensors by Aptamer Engineering and Toehold‐Mediated Strand Displacement

Advanced Science, EarlyView.
Aptamer‐based single‐walled carbon nanotube (SWCNT) field‐effect transistor (FET) biosensors are developed for label‐free, real‐time biomarker detection. Truncated DNA aptamers enhance sensitivity through improved conformational switching. A toehold‐mediated strand displacement (TMSD) strategy optimizes functionalization and simplifies fabrication. The
Haosen Miao, Tingting Zheng, Houlin Yu, Gririraj Manoharan, Gustavo Sant'Anna, Nicholas Bedfrod, Jorge L. Chávez, Chang‐Seuk Lee, Matteo Palma   +8 more
wiley   +1 more source

Mathematical Estimation of Logical Masking Capability of Majority/Minority Gates Used in Nanoelectronic Circuits

, 2017
In nanoelectronic circuit synthesis, the majority gate and the inverter form the basic combinational logic primitives. This paper deduces the mathematical formulae to estimate the logical masking capability of majority gates, which are used extensively ...
Balasubramanian, P, Naayagi, R T
core   +1 more source

Transcriptional regulation by competing transcription factor modules. [PDF]

PLoS Computational Biology, 2006
Gene regulatory networks lie at the heart of cellular computation. In these networks, intracellular and extracellular signals are integrated by transcription factors, which control the expression of transcription units by binding to cis-regulatory ...
Rutger Hermsen, Sander Tans, Pieter Rein ten Wolde   +2 more
doaj   +1 more source

Deep Learning‐Assisted Design of Mechanical Metamaterials

Advanced Intelligent Discovery, EarlyView.
This review examines the role of data‐driven deep learning methodologies in advancing mechanical metamaterial design, focusing on the specific methodologies, applications, challenges, and outlooks of this field. Mechanical metamaterials (MMs), characterized by their extraordinary mechanical behaviors derived from architected microstructures, have ...
Zisheng Zong, Pu Wen, Zhiping Chai, Dunxuan Wu, Han Ding, Zhigang Wu   +5 more
wiley   +1 more source