Results 11 to 20 of about 1,176,556 (303)

RanBP2-Mediated SUMOylation Promotes Human DNA Polymerase Lambda Nuclear Localization and DNA Repair [PDF]

, 2020
Cellular DNA is under constant attack by a wide variety of agents, both endogenous and exogenous. To counteract DNA damage, human cells have a large collection of DNA repair factors. Among them, DNA polymerase lambda (Polλ) stands out for its versatility,
Cortés Ledesma, Felipe, García Domínguez, Mario, Herrero Ruiz, Andrés M., Moreno Oñate, Marta, Ruiz Pérez, José Francisco   +4 more
core   +1 more source

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair. [PDF]

, 2020
Repair of double strand DNA breaks (DSBs) can result in gene disruption or gene modification via homology directed repair (HDR) from donor DNA. Altering cellular responses to DSBs may rebalance editing outcomes towards HDR and away from other repair ...
A Ferreira, A Montagnoli, A Orthwein, AC Komor, B Roberts, B Verdoodt, C Anders, C Yu, CD Richardson, CD Richardson, D Szklarczyk, DW Huang, E Lingeman, ES Koltun, FK Zimmermann, H Ghezraoui, J Song, JA Doudna, JE Haber, JP Duxin, K Jayathilaka, K Nakanishi, K Okita, KJ Livak, L Lei, L Pessoa-Brandão, LA Gilbert, LN Brandão, M Cavazzana, M Lei, M Yamada, MA DeWitt, MD Canny, MD Rainey, MH Porteus, N Hustedt, N Zielke, NK Sasi, NR Pannunzio, R Ceccaldi, RC Alver, S Hughes, S Lin, SB Thyme, T Maruyama, T Takenaka, T Tsuji, TA Day, TL Roth, TS Nambiar, U Eichenlaub-Ritter, VT Chu, W Li, X Liang, Z Romero   +54 more
core   +1 more source

5hmC modification regulates R-loop accumulation in response to stress

Frontiers in Psychiatry, 2023
R-loop, an RNA-DNA hybrid structure, arises as a transcriptional by-product and has been implicated in DNA damage and genomic instability when excessive R-loop is accumulated. Although previous study demonstrated that R-loop is associated with ten-eleven
Xingyun Xu, Xingyun Xu, Junjie Wang, Wenjuan Wang, Yutong Zhang, Bo Wan, Zhigang Miao, Xingshun Xu, Xingshun Xu, Xingshun Xu   +9 more
doaj   +1 more source

Modification of Subtelomeric DNA [PDF]

Molecular and Cellular Biology, 2004
There is a discrepancy in telomere length as measured by signal intensity of telomere restriction fragments on gels and fluorescence in situ hybridization analysis. This difference has been ascribed to the X-region, a segment of subtelomeric DNA that is resistant to being cut by restriction enzymes. To explore the nature of this region, we analyzed the
Susanne, Steinert, Jerry W, Shay, Woodring E, Wright   +2 more
openaire   +2 more sources

Alleviation of C⋅C Mismatches in DNA by the Escherichia coli Fpg Protein

Frontiers in Microbiology, 2021
DNA polymerase III mis-insertion may, where not corrected by its 3′→ 5′ exonuclease or the mismatch repair (MMR) function, result in all possible non-cognate base pairs in DNA generating base substitutions.
Almaz Nigatu Tesfahun, Marina Alexeeva, Miglė Tomkuvienė, Aysha Arshad, Prashanna Guragain, Arne Klungland, Arne Klungland, Saulius Klimašauskas, Peter Ruoff, Svein Bjelland, Svein Bjelland   +10 more
doaj   +1 more source

Genetic Modification of Sodalis Species by DNA Transduction

mSphere, 2021
A large number of economically important insects maintain intimate associations with maternally inherited endosymbiotic bacteria. Due to the inherent nature of these associations, insect endosymbionts cannot be usually isolated in pure culture or ...
Chelsea M. Keller, Christopher G. Kendra, Roberto E. Bruna, David Craft, Mauricio H. Pontes   +4 more
doaj   +1 more source

DNA N6-Methyladenine Modification in Eukaryotic Genome

Frontiers in Genetics, 2022
DNA methylation is treated as an important epigenetic mark in various biological activities. In the past, a large number of articles focused on 5 mC while lacking attention to N6-methyladenine (6 mA).
Hao Li, Hao Li, Hao Li, Ning Zhang, Ning Zhang, Ning Zhang, Yuechen Wang, Yuechen Wang, Siyuan Xia, Siyuan Xia, Yating Zhu, Chen Xing, Xuefeng Tian, Xuefeng Tian, Yinan Du   +14 more
doaj   +1 more source

Allele-Specific PCR for PIK3CA Mutation Detection Using Phosphoryl Guanidine Modified Primers

Diagnostics, 2023
Phosphoryl guanidine (PG) is the novel uncharged modification of internucleotide phosphates of oligonucleotides. Incorporating PG modification into PCR primers leads to increased discrimination between wild-type and mutated DNA, providing extraordinary ...
Alexey S. Chubarov, Igor P. Oscorbin, Lidiya M. Novikova, Maxim L. Filipenko, Alexander A. Lomzov, Dmitrii V. Pyshnyi   +5 more
doaj   +1 more source

The extremophile Picrophilus torridus carries a DNA adenine methylase M.PtoI that is part of a Type I restriction-modification system

Frontiers in Microbiology, 2023
DNA methylation events mediated by orphan methyltransferases modulate various cellular processes like replication, repair and transcription. Bacteria and archaea also harbor DNA methyltransferases that are part of restriction-modification systems, which ...
Pallavi Gulati, Ashish Singh, Manisha Goel, Swati Saha   +3 more
doaj   +1 more source

Ethics of modifying the mitochondrial genome [PDF]

, 2010
Recent preclinical studies have shown the feasibility of specific variants of nuclear transfer to prevent mitochondrial DNA disorders. Nuclear transfer could be a valuable reproductive option for carriers of mitochondrial mutations.
Bredenoord, A L, de Wert, G, Dondorp, W, Pennings, Guido   +3 more
core   +3 more sources