Results 11 to 20 of about 1,473,978 (184)
Bacteriophage T7 DNA polymerase – sequenase [PDF]
Frontiers in Microbiology, 2014 An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: (1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; (2) high processivity; (3) high ...
Zhu, Bin
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Nobel Prize laureate Kary Mullis and the polymerase chain reaction (PCR) [PDF]
The Ukrainian Biochemical Journal, 2021 The article highlights the major life and career milestones and the extraordinary personality of 1993 Nobel Prize laureate in Chemistry Kary B. Mullis.
V. M. Danilova +2 more
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Frontiers in Bioengineering and Biotechnology, 2021 A change of an aspartic acid to asparagine of Taq (Thermus aquaticus) DNA polymerase is a gain of function mutation that supports faster PCR: the extension times for PCR amplification can be 2–3 times shorter.
Wayne M. Barnes +3 more
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Interactions of the Bacillus subtilis DnaE polymerase with replisomal proteins modulate its activity and fidelity [PDF]
Open Biology, 2017 During Bacillus subtilis replication two replicative polymerases function at the replisome to collectively carry out genome replication. In a reconstituted in vitro replication assay, PolC is the main polymerase while the lagging strand DnaE polymerase ...
Vasileios Paschalis +5 more
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International Journal of Technology, 2020 Access to biological engineering has become a critical point of modern science development through polymerase chain reaction (PCR). One of the main components in this process is DNA polymerase, which copies the main template DNA.
Kenny Lischer +5 more
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Jurnal Entomologi Indonesia, 2022 Identification based on molecular character of Nucleopolyhedrovirusin Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) larvae from Bogor, West Java.
R. Yayi Munara Kusumah +2 more
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DNA polymerase III protein, HolC, helps resolve replication/transcription conflicts
Microbial Cell, 2021 In Escherichia coli, DNA replication is catalyzed by an assembly of proteins, the DNA polymerase III holoenzyme. This complex includes the polymerase and proofreading subunits, the processivity clamp and clamp loader complex.
Susan T. Lovett
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The human CTF4-orthologue AND-1 interacts with DNA polymerase α/primase via its unique C-terminal HMG box [PDF]
Open Biology, 2017 A dynamic multi-protein assembly known as the replisome is responsible for DNA synthesis in eukaryotic cells. In yeast, the hub protein Ctf4 bridges DNA helicase and DNA polymerase and recruits factors with roles in metabolic processes coupled to DNA ...
Mairi L. Kilkenny +5 more
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Live culture-based qPCR screening of Taq DNA polymerase variants for resistance to PCR inhibitors. [PDF]
Front Bioeng BiotechnolWe present a live culture PCR (LC-PCR) workflow that enables direct screening of randomly mutagenized Thermus aquaticus (Taq) and Klentaq1 DNA polymerase libraries without enzyme purification.
Kermekchiev MB, Zhang Z, Barnes WM.
europepmc +2 more sources
Jurnal Kimia Sains dan Aplikasi, 2020 The search for novel DNA Polymerases I, with higher fidelity and better polymerization rate, is essential to improve the Polymerase Chain Reaction method.
Teuku Mohamad Iqbalsyah +3 more
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