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Current Protocols in Molecular Biology, 2008
AbstractThis unit presents characteristics and reaction conditions of the DNA‐dependent DNA polymerases, including E. coli DNA polymerase I and its Klenow fragment, T4 DNA polymerase, native and modified T7 DNA polymerase, phi29 DNA polymerase, Bst DNA polymerase, and Taq DNA polymerase. The unit also provides overviews of other classes of thermophilic
Rebecca Kucera, Nicole M. Nichols
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AbstractThis unit presents characteristics and reaction conditions of the DNA‐dependent DNA polymerases, including E. coli DNA polymerase I and its Klenow fragment, T4 DNA polymerase, native and modified T7 DNA polymerase, phi29 DNA polymerase, Bst DNA polymerase, and Taq DNA polymerase. The unit also provides overviews of other classes of thermophilic
Rebecca Kucera, Nicole M. Nichols
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Biochemical and Biophysical Research Communications, 1971
Summary Homogeneous DNA polymerases from E. coli and sea urchins contain zinc in a proportion of approximately 2 and 4 gm atoms Zn/mole enzyme respectively. Specific inhibition of the enzyme by ortho -phenanthroline and lack of inhibition by meta -phenanthroline suggest that the bound zinc plays a functional role in the interaction of DNA with ...
Albert S. Mildvan +2 more
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Summary Homogeneous DNA polymerases from E. coli and sea urchins contain zinc in a proportion of approximately 2 and 4 gm atoms Zn/mole enzyme respectively. Specific inhibition of the enzyme by ortho -phenanthroline and lack of inhibition by meta -phenanthroline suggest that the bound zinc plays a functional role in the interaction of DNA with ...
Albert S. Mildvan +2 more
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Nature, 1974
DNA polymerase has been isolated from the cytoplasm of several mammalian tissues as well as several cell lines in culture1–8. This has been puzzling because DNA replication and repair are processes generally believed to be restricted to the nucleus, where chromatin is located.
Kathleen M. Downey +3 more
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DNA polymerase has been isolated from the cytoplasm of several mammalian tissues as well as several cell lines in culture1–8. This has been puzzling because DNA replication and repair are processes generally believed to be restricted to the nucleus, where chromatin is located.
Kathleen M. Downey +3 more
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Current Opinion in Structural Biology, 2018
The eukaryotic DNA replication machinery is conserved from yeast to humans and requires the actions of multiple DNA polymerases. In addition to replicative DNA polymerases for duplication of the leading and lagging DNA strands, another group of specialized polymerases is required for DNA repair and/or translesion DNA synthesis (TLS).
Aneel K. Aggarwal +2 more
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The eukaryotic DNA replication machinery is conserved from yeast to humans and requires the actions of multiple DNA polymerases. In addition to replicative DNA polymerases for duplication of the leading and lagging DNA strands, another group of specialized polymerases is required for DNA repair and/or translesion DNA synthesis (TLS).
Aneel K. Aggarwal +2 more
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DNA polymerases and carcinogenesis
Biochemistry (Moscow), 2010There are many various chromosomal and gene mutations in human cancer cells. The total mutation rate in normal human cells is 2·10(-7) mutations/gene/division. From 6 to 12 carcinogenic mutations can arise by the end of the life, and these can affect the structure of ~150 protooncogenes and genes encoding suppressors of tumor growth. However, this does
T. P. Kravetskaya, V. M. Krutyakov
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Annual Review of Biochemistry, 1977
PERSPECTIVES A ND SU MMARY ......... 25 IN TR ODUCTION ........ ... 27 CE LLU LAR DNA POL YMERASES ....... . 28 DNA Polymerase a ......... 28 DNA Polymerase /3 31 DNA Polymerase 'Y •.....• • 33 Mitochondrial DNA Polymerase .... ......... 34 VIR US-INDUCED DNA POL YMERASES ......... ....
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PERSPECTIVES A ND SU MMARY ......... 25 IN TR ODUCTION ........ ... 27 CE LLU LAR DNA POL YMERASES ....... . 28 DNA Polymerase a ......... 28 DNA Polymerase /3 31 DNA Polymerase 'Y •.....• • 33 Mitochondrial DNA Polymerase .... ......... 34 VIR US-INDUCED DNA POL YMERASES ......... ....
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Nature, 1970
A membrane associated DNA polymerizing enzyme has been solubilized, partially isolated and characterized.
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A membrane associated DNA polymerizing enzyme has been solubilized, partially isolated and characterized.
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Current Protocols in Molecular Biology, 2012
AbstractThe DNA Polymerase Database (Polbase) is intended to compile the wealth of existing DNA polymerase information from public and private records into an open, searchable database. Curr. Protoc. Mol. Biol. 99:3.25.1‐3.25.2. © 2012 by John Wiley & Sons, Inc.
Nicole M. Nichols, Bradley W. Langhorst
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AbstractThe DNA Polymerase Database (Polbase) is intended to compile the wealth of existing DNA polymerase information from public and private records into an open, searchable database. Curr. Protoc. Mol. Biol. 99:3.25.1‐3.25.2. © 2012 by John Wiley & Sons, Inc.
Nicole M. Nichols, Bradley W. Langhorst
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[8] Purification of mammalian DNA polymerases: DNA polymerase α
1995Publisher Summary This chapter discusses the purification of mammalian DNA polymerases, DNA polymerase α. DNA polymerase α plays a key role in the initiation and replication of the mammalian chromosome. The production of a panel of monoclonal antibodies against human DNA polymerase α allowed the development of a three-step immunoaffinity purification
Lars Rogge +3 more
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Biochemical and Biophysical Research Communications, 1965
Abstract An experiment will be described which shows that DNA polymerase helps select the base in DNA replication. After elucidating the structure of DNA (1953a), Watson and Crick proposed a template mechanism for the replication of DNA (1953b).
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Abstract An experiment will be described which shows that DNA polymerase helps select the base in DNA replication. After elucidating the structure of DNA (1953a), Watson and Crick proposed a template mechanism for the replication of DNA (1953b).
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