Results 21 to 30 of about 945,929 (245)
Engineering processive DNA polymerases with maximum benefit and minimum cost
Frontiers in Microbiology, 2014 DNA polymerases need to be engineered to achieve optimal performance for biotechnological applications, which often require high fidelity replication when using modified nucleotides and when replicating difficult DNA sequences.
Linda J. Reha-Krantz +2 more
doaj +1 more source
Preparation of Phi29 DNA polymerase free of amplifiable DNA using ethidium monoazide, an ultraviolet-free light-emitting diode lamp and trehalose. [PDF]
PLoS ONE, 2014 We previously reported that multiply-primed rolling circle amplification (MRPCA) using modified random RNA primers can amplify tiny amounts of circular DNA without producing any byproducts.
Hirokazu Takahashi +10 more
doaj +1 more source
Direct Enzyme Engineering of B Family DNA Polymerases for Biotechnological Approaches
Bioengineering, 2023 DNA-dependent DNA polymerases have been intensively studied for more than 60 years and underlie numerous biotechnological and diagnostic applications.
Aleksandra A. Kuznetsova +1 more
doaj +1 more source
Brazilian Journal of Medical and Biological Research, 1998 For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that
R.S. Diaz, E.C. Sabino
doaj +1 more source
A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification
Iranian Journal of Microbiology, 2019 Background and Objectives: Pfu DNA polymerase is an enzyme that exhibits the lowest error rate in the 3´ to 5´ exonuclease (proofreading) activity during DNA synthesis in Polymerase Chain Reactions (PCRs).
Prabu Siva Sankar +6 more
doaj +1 more source
Bst polymerase — a humble relative of Taq polymerase
Computational and Structural Biotechnology Journal, 2023 DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair.
Igor Oscorbin, Maxim Filipenko
doaj +1 more source
Bacteriophage T7 DNA polymerase — Sequenase
Frontiers in Microbiology, 2014 An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: 1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; 2) high processivity; 3) high ...
Bin eZhu
doaj +1 more source
Scientific Reports, 2017 Organisms possess multiple DNA polymerases (Pols) and use each for a different purpose. One of the five Pols in Escherichia coli, DNA polymerase IV (Pol IV), encoded by the dinB gene, is known to participate in lesion bypass at certain DNA adducts.
Thanh Thi Le +3 more
doaj +1 more source
Fidelity of DNA polymerases in the detection of intraindividual variation of mitochondrial DNA
Mitochondrial DNA. Part B. Resources, 2020 Here we investigated the consequences of PCR amplification errors in the identification of intraindividual mtDNA variation. The bumblebee Bombus morio was chosen as model for the COI gene amplification tests with two DNA polymerases (Taq and Q5 ...
Paulo Cseri Ricardo +2 more
doaj +1 more source
Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source