Results 31 to 40 of about 894,362 (295)
DNA polymerases and cancer [PDF]
Nature Reviews Cancer, 2011 There are 15 different DNA polymerases encoded in mammalian genomes, which are specialized for replication, repair or the tolerance of DNA damage. New evidence is emerging for lesion-specific and tissue-specific functions of DNA polymerases. Many point mutations that occur in cancer cells arise from the error-generating activities of DNA polymerases ...
Sabine S. Lange +2 more
openaire +3 more sources
Brazilian Journal of Medical and Biological Research, 1998 For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that
R.S. Diaz, E.C. Sabino
doaj +1 more source
A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification
Iranian Journal of Microbiology, 2019 Background and Objectives: Pfu DNA polymerase is an enzyme that exhibits the lowest error rate in the 3´ to 5´ exonuclease (proofreading) activity during DNA synthesis in Polymerase Chain Reactions (PCRs).
Prabu Siva Sankar +6 more
doaj +1 more source
Bacteriophage T7 DNA polymerase — Sequenase
Frontiers in Microbiology, 2014 An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: 1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; 2) high processivity; 3) high ...
Bin eZhu
doaj +1 more source
Fidelity of DNA polymerases in the detection of intraindividual variation of mitochondrial DNA
Mitochondrial DNA. Part B. Resources, 2020 Here we investigated the consequences of PCR amplification errors in the identification of intraindividual mtDNA variation. The bumblebee Bombus morio was chosen as model for the COI gene amplification tests with two DNA polymerases (Taq and Q5 ...
Paulo Cseri Ricardo +2 more
doaj +1 more source
Scientific Reports, 2017 Organisms possess multiple DNA polymerases (Pols) and use each for a different purpose. One of the five Pols in Escherichia coli, DNA polymerase IV (Pol IV), encoded by the dinB gene, is known to participate in lesion bypass at certain DNA adducts.
Thanh Thi Le +3 more
doaj +1 more source
A Well-Conserved Archaeal B-Family Polymerase Functions as an Extender in Translesion Synthesis
mBio, 2022 B-family DNA polymerases (PolBs) of different groups are widespread in Archaea, and different PolBs often coexist in the same organism. Many of these PolB enzymes remain to be investigated.
Xu Feng +9 more
doaj +1 more source
Translesion DNA Polymerases [PDF]
Cold Spring Harbor Perspectives in Biology, 2013 Living cells are continually exposed to DNA-damaging agents that threaten their genomic integrity. Although DNA repair processes rapidly target the damaged DNA for repair, some lesions nevertheless persist and block genome duplication by the cell's replicase.
Roger Woodgate, Myron F. Goodman
openaire +2 more sources
Fluorescence-Based, High-Throughput DNA Polymerase Assay
BioTechniques, 2002 The commonly used DNA polymerase assay is based on the detection of incorporated radiolabeled nucleotides in a DNA elongation reaction. It is laborious, radioactive, and can be highly variable.
Liming Yu, Guolu Hu, Leighton Howells
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Social context prevents heat hormetic effects against mutagens during fish development
FEBS Letters, EarlyView.This study shows that sublethal heat stress protects fish embryos against ultraviolet radiation, a concept known as ‘hormesis’. However, chemical stress transmission between fish embryos negates this protective effect. By providing evidence for the mechanistic molecular basis of heat stress hormesis and interindividual stress communication, this study ...
Lauric Feugere +5 more
wiley +1 more source