Cyclocytidine hydrochloride inhibits the synthesis of relaxed circular DNA of hepatitis B virus [PDF]
PeerJ, 2022 Background Cyclocytidine hydrochloride (HCl) has been reported to inhibit DNA synthesis by affecting DNA polymerase. Here, we tested the antiviral effect of cyclocytidine on hepatitis B virus (HBV) DNA synthesis, which is reliant on DNA polymerase ...
Xue Wang +7 more
doaj +2 more sources
Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates [PDF]
, 2013 Nonhomologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although it is not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA
Bartlett, Edward +2 more
core +1 more source
DNA Polymerase Profiling [PDF]
, 2008 We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high throughput. The fluorescence signal is generated by the DNA polymerase triggering opening of a molecular beacon extension of the template strand. A resulting distance alteration is reported by fluorescence resonance energy transfer between two dyes ...
openaire +3 more sources
Promutagenicity of 8-Chloroguanine, A Major Inflammation-Induced Halogenated DNA Lesion
Molecules, 2019 Chronic inflammation is closely associated with cancer development. One possible mechanism for inflammation-induced carcinogenesis is DNA damage caused by reactive halogen species, such as hypochlorous acid, which is released by myeloperoxidase to kill ...
Yi Kou, Myong-Chul Koag, Seongmin Lee
doaj +1 more source
Molecular dissection of the domain architecture and catalytic activities of human PrimPol [PDF]
, 2014 PrimPol is a primase–polymerase involved in nuclear and mitochondrial DNA replication in eukaryotic cells. Although PrimPol is predicted to possess an archaeo-eukaryotic primase and a UL52-like zinc finger domain, the role of these domains has not been ...
Aidan J. Doherty +45 more
core +2 more sources
DNA Polymerase e - More Than a Polymerase
The Scientific World Journal, 2003 This paper presents a comprehensive review of the structure and function of DNA polymerase e. Together with DNA polymerases a and d, this enzyme replicates the nuclear DNA in the eukaryotic cell.
Helmut Pospiech, Juhani E. Syväoja
doaj +1 more source
A nuclear family A DNA polymerase from Entamoeba histolytica bypasses thymine glycol. [PDF]
PLoS Neglected Tropical Diseases, 2010 BACKGROUND: Eukaryotic family A DNA polymerases are involved in mitochondrial DNA replication or translesion DNA synthesis. Here, we present evidence that the sole family A DNA polymerase from the parasite protozoan E.
Guillermo Pastor-Palacios +2 more
doaj +1 more source
Visualizing DNA polymerase ι catalyze Hoogsteen-directed DNA synthesis. [PDF]
Nat CommunTranslesion synthesis polymerases efficiently incorporate nucleotides opposite DNA lesions. Pol ι, for example, bypasses minor-groove and exocyclic purine adducts.
Frevert Z +6 more
europepmc +2 more sources
Exploration of factors driving incorporation of unnatural dNTPS into DNA by Klenow fragment (DNA polymerase I) and DNA polymerase {alpha} [PDF]
, 2006 In order to further understand how DNA polymerases discriminate against incorrect dNTPs, we synthesized two sets of dNTP analogues and tested them as substrates for DNA polymerase a (pol alpha) and Klenow fragment (exo-) of DNA polymerase I (Escherichia ...
Kincaid, Kristi +5 more
core +1 more source
Engineering processive DNA polymerases with maximum benefit and minimum cost
Frontiers in Microbiology, 2014 DNA polymerases need to be engineered to achieve optimal performance for biotechnological applications, which often require high fidelity replication when using modified nucleotides and when replicating difficult DNA sequences.
Linda J. Reha-Krantz +2 more
doaj +1 more source