Results 41 to 50 of about 43,846 (273)
A Well-Conserved Archaeal B-Family Polymerase Functions as an Extender in Translesion Synthesis
mBio, 2022 B-family DNA polymerases (PolBs) of different groups are widespread in Archaea, and different PolBs often coexist in the same organism. Many of these PolB enzymes remain to be investigated.
Xu Feng +9 more
doaj +1 more source
Scientific Reports, 2017 Organisms possess multiple DNA polymerases (Pols) and use each for a different purpose. One of the five Pols in Escherichia coli, DNA polymerase IV (Pol IV), encoded by the dinB gene, is known to participate in lesion bypass at certain DNA adducts.
Thanh Thi Le +3 more
doaj +1 more source
Fluorescence-Based, High-Throughput DNA Polymerase Assay
BioTechniques, 2002 The commonly used DNA polymerase assay is based on the detection of incorporated radiolabeled nucleotides in a DNA elongation reaction. It is laborious, radioactive, and can be highly variable.
Liming Yu, Guolu Hu, Leighton Howells
doaj +1 more source
Translesion DNA Polymerases [PDF]
Cold Spring Harbor Perspectives in Biology, 2013 Living cells are continually exposed to DNA-damaging agents that threaten their genomic integrity. Although DNA repair processes rapidly target the damaged DNA for repair, some lesions nevertheless persist and block genome duplication by the cell's replicase.
Roger Woodgate, Myron F. Goodman
openaire +2 more sources
Error correction during DNA replication [PDF]
Physical Review E, VOL.86, 011913 (2012), 2011 DNA polymerase (DNAP) is a dual-purpose enzyme that plays two opposite roles in two different situations during DNA replication. It plays its normal role as a {\it polymerase} catalyzing the elongation of a new DNA molecule by adding a monomer. However, it can switch to the role of an {\it exonuclease} and shorten the same DNA by cleavage of the last ...
arxiv +1 more source
Bacteriophage T7 DNA polymerase — Sequenase
Frontiers in Microbiology, 2014 An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: 1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; 2) high processivity; 3) high ...
Bin eZhu
doaj +1 more source
Taurine promotes glucagon‐like peptide‐1 secretion in enteroendocrine L cells
FEBS Letters, EarlyView.Taurine, a sulfur‐containing amino acid, is likely taken up by enteroendocrine L cells via the taurine transporter. This process increases the levels of cytosolic ATP. The increase in intracellular Ca2+ concentrations and glucagon‐like peptide‐1 secretion through membrane depolarization is caused by the closure of ATP‐sensitive potassium channels ...
Yuri Osuga +6 more
wiley +1 more source
FEBS Letters, EarlyView.The S1025 peptide is the major antidote to the YrzI toxin, which we renamed here as SpyT (Small Peptide YrzI Toxin) and SpyA (Small Peptide YrzI Antitoxin) (1). Degradation of the toxin–antitoxin spyTA mRNA, either by a translation‐dependent cleavage by the endoribonuclease Rae1 (2) or by direct attack by 3′‐exoribonucleases (3), also contributes to ...
Laetitia Gilet +4 more
wiley +1 more source
Mechanical Properties of Transcription [PDF]
Phys. Rev. Lett. 118, 268101 (2017), 2016 Recently the physical characterization of a number of biological processes has proven indispensable for a full understanding of natural phenomena. One such example is the mechanical properties of transcription, which have been shown to have significant effects in gene expression.
arxiv +1 more source
Molecular Oncology, EarlyView.Circulating tumor DNA (ctDNA) offers a possibility for different applications in early and late stage breast cancer management. In early breast cancer tumor informed approaches are increasingly used for detecting molecular residual disease (MRD) and early recurrence. In advanced stage, ctDNA provides a possibility for monitoring disease progression and
Eva Valentina Klocker +14 more
wiley +1 more source