Preparation of Phi29 DNA polymerase free of amplifiable DNA using ethidium monoazide, an ultraviolet-free light-emitting diode lamp and trehalose. [PDF]
PLoS ONE, 2014 We previously reported that multiply-primed rolling circle amplification (MRPCA) using modified random RNA primers can amplify tiny amounts of circular DNA without producing any byproducts.
Hirokazu Takahashi +10 more
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Characterization of RNA polymerases from Rous sarcoma virus-induced mouse ascites sarcoma cells. [PDF]
, 1979 RNA polymerase was extracted from the Schmidt-Ruppin strain of Rous sarcoma virus (SR-RSV)-induced C3H/He mouse ascites sarcoma cells (SR-C3H). RNA polymerase was separated into RNA polymerases I and II by DEAE-Sephadex chromatography.
Misumi, Hiromasa, Oda, Takuzo
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Replicative DNA Polymerases [PDF]
Cold Spring Harbor Perspectives in Biology, 2013 In 1959, Arthur Kornberg was awarded the Nobel Prize for his work on the principles by which DNA is duplicated by DNA polymerases. Since then, it has been confirmed in all branches of life that replicative DNA polymerases require a single-stranded template to build a complementary strand, but they cannot start a new DNA strand de novo.
Johansson, Erik, Dixon, Nicholas
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Caenorhabditis elegans as a model system for studying drug induced mitochondrial toxicity [PDF]
, 2015 Today HIV-1 infection is recognized as a chronic disease with obligatory lifelong treatment to keep viral titers below detectable levels. The continuous intake of antiretroviral drugs however, leads to severe and even life-threatening side effects ...
Brul, Stanley +5 more
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Plasmid-based lacZalpha assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase [PDF]
, 2009 The preparation of a gapped pUC18 derivative, containing the lacZalpha reporter gene in the single-stranded region, is described. Gapping is achieved by flanking the lacZalpha gene with sites for two related nicking endonucleases, enabling the excision ...
Bebenek +28 more
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Direct Enzyme Engineering of B Family DNA Polymerases for Biotechnological Approaches
Bioengineering, 2023 DNA-dependent DNA polymerases have been intensively studied for more than 60 years and underlie numerous biotechnological and diagnostic applications.
Aleksandra A. Kuznetsova +1 more
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Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates [PDF]
, 2013 Nonhomologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although it is not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA
Bartlett, Edward +2 more
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Brazilian Journal of Medical and Biological Research, 1998 For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that
R.S. Diaz, E.C. Sabino
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A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification
Iranian Journal of Microbiology, 2019 Background and Objectives: Pfu DNA polymerase is an enzyme that exhibits the lowest error rate in the 3´ to 5´ exonuclease (proofreading) activity during DNA synthesis in Polymerase Chain Reactions (PCRs).
Prabu Siva Sankar +6 more
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Bst polymerase — a humble relative of Taq polymerase
Computational and Structural Biotechnology Journal, 2023 DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair.
Igor Oscorbin, Maxim Filipenko
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