Results 41 to 50 of about 225,059 (304)

Fumarase affects the deoxyribonucleic acid damage response by protecting the mitochondrial desulfurase Nfs1p from modification and inactivation

iScience, 2021
Summary: The Krebs cycle enzyme fumarase, which has been identified as a tumor suppressor, is involved in the deoxyribonucleic acid (DNA) damage response (DDR) in human, yeast, and bacterial cells.
Joyce Yip, Suqing Wang, Jasper Tan, Teck Kwang Lim, Qingsong Lin, Zhang Yu, Ofri Karmon, Ophry Pines, Norbert Lehming   +8 more
doaj   +1 more source

Visualizing helicases unwinding DNA at the single molecule level. [PDF]

, 2010
DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free energy from ATP hydrolysis. Single molecule approaches enable us to address detailed mechanistic questions about how such enzymes ...
Fili, Natali, Mashanov, Gregory I, Batters, C, Dillingham, MS, Yeeles, Joseph T P, Toseland, Christopher P, Mashanov, GI, Toseland, CP, Wallace, Mark I, Webb, Martin R, Molloy, JE, Batters, Christopher, Webb, MR, Wallace, Mark, Fili, N, Dillingham, Mark S, Yeeles, JT, Molloy, Justin E   +17 more
core   +1 more source

Oxidative stress and 8-oxoguanine repair are enhanced in colon adenoma and carcinoma patients. [PDF]

, 2010
Oxidative stress is involved in the pathogenesis of colon cancer. We wanted to elucidate at which stage of the disease this phenomenon occurs. In the examined groups of patients with colorectal cancer (CRC, n = 89), benign adenoma (AD, n = 77) and ...
R. Rozalski, A. Szpila, B. Tudek, Speina, Elzbieta, Oliński, Ryszard, Janowska, Beata, J. Janik, Obtułowicz, Tomasz, Janik, Justyna, T. Dziaman, Dziaman, Tomasz, Szpila, Anna, J. M. Ciesla, Siomek, Agnieszka, A. Siomek, Cieśla, Jarosław M., Jawien, Arkadiusz, Gackowski, Daniel, M. Swoboda, E. Speina, R. Olinski, Swoboda, Maja, D. Gackowski, Guz, Jolanta, B. Janowska, J. Guz, Z. Banaszkiewicz, A. Jawien, Rozalski, Rafał, T. Obtulowicz, Tudek, Barbara, Banaszkiewicz, Zbigniew   +31 more
core   +1 more source

Investigating the regulation of ZUP1, a novel deubiquitinating enzyme involved in DNA repair, by coupled monoubiquitination

, 2021
Ubiquitination, a major post-translational modification, is reversed through the action of a large family of specialised proteases termed deubiquitinating enzymes (DUBs).
Schützenhofer, Kira
core   +1 more source

Development of a rapid electrophoretic assay for genomic DNA damage quantification

Ecotoxicology and Environmental Safety, 2021
Accuracy, sensitivity, simplicity, reproducibility, and low-cost are desirable requirements for genotoxicity assessment techniques. Here we describe a simple electrophoretic assay for genomic DNA lesions quantification (EAsy-GeL) based on subjecting DNA ...
James Eduardo Lago Londero, Cassiano Ricardo Schavinski, Fernanda D'Avila da Silva, Bruna Candia Piccoli, André Passaglia Schuch   +4 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Repair of DNA interstrand crosslinks as a mechanism of clinical resistance to melphalan in multiple myeloma

, 2002
Melphalan is widely used as a preparative agent in patients with multiple myeloma (MM) undergoing autologous stem cell transplantation (SCT). Although disease relapse is the major cause of death after a melphalan-conditioned autograft, the mechanism ...
Mahendra, P, Hochhauser, D, Hockhauser, D, Spanswick, VJ, Shankaranarayana, P, Mahendra, Premini, Hughes, RG, Craddock, Charles; id_orcid, Craddock, C, Sekhar, M, Hartley, JA, Spanswick, V   +11 more
core   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

DNA damage shifts circadian clock time via Hausp-dependent Cry1 stabilization

eLife, 2015
The circadian transcriptional repressors cryptochrome 1 (Cry1) and 2 (Cry2) evolved from photolyases, bacterial light-activated DNA repair enzymes. In this study, we report that while they have lost DNA repair activity, Cry1/2 adapted to protect genomic ...
Stephanie J Papp, Anne-Laure Huber, Sabine D Jordan, Anna Kriebs, Madelena Nguyen, James J Moresco, John R Yates III, Katja A Lamia   +7 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source