Results 61 to 70 of about 225,059 (304)

TopBP1 utilises a bipartite GINS binding mode to support genome replication

Nature Communications
Activation of the replicative Mcm2-7 helicase by loading GINS and Cdc45 is crucial for replication origin firing, and as such for faithful genetic inheritance.
Matthew Day, Bilal Tetik, Milena Parlak, Yasser Almeida-Hernández, Markus Räschle, Farnusch Kaschani, Heike Siegert, Anika Marko, Elsa Sanchez-Garcia, Markus Kaiser, Isabel A. Barker, Laurence H. Pearl, Antony W. Oliver, Dominik Boos   +13 more
doaj   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

DNA–protein crosslink repair: proteases as DNA repair enzymes

Trends in Biochemical Sciences, 2015
DNA-protein crosslinks (DPCs) are highly toxic DNA lesions because they interfere with DNA transactions. The recent discovery of a yeast protease that processes DPCs proteolytically raises the question whether DPC proteases also exist in higher eukaryotes.
Julian Stingele, Bianca Habermann, Stefan Jentsch   +2 more
openaire   +4 more sources

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

Accessing DNA damage in chromatin: Preparing the chromatin landscape for base excision repair

, 2015
DNA damage in chromatin comes in many forms, including single base lesions that induce base excision repair (BER). We and others have shown that the structural location of DNA lesions within nucleosomes greatly influences their accessibility to repair ...
Rodriguez, Yesenia, Hinz, John M, Smerdon, Michael J   +2 more
core   +1 more source

No cancer predisposition or increased spontaneous mutation frequencies in NEIL DNA glycosylases-deficient mice

Scientific Reports, 2017
Base excision repair (BER) is a major pathway for removal of DNA base lesions and maintenance of genomic stability, which is essential in cancer prevention. DNA glycosylases recognize and remove specific lesions in the first step of BER. The existence of
Veslemøy Rolseth, Luisa Luna, Ann Karin Olsen, Rajikala Suganthan, Katja Scheffler, Christine G. Neurauter, Ying Esbensen, Anna Kuśnierczyk, Gunn A. Hildrestrand, Anne Graupner, Jill M. Andersen, Geir Slupphaug, Arne Klungland, Hilde Nilsen, Magnar Bjørås   +14 more
doaj   +1 more source

Mechanism for CCC DNA synthesis in hepadnaviruses. [PDF]

PLoS ONE, 2009
Hepadnavirus replication requires the synthesis of a covalently closed circular (CCC) DNA from the relaxed circular (RC) viral genome by an unknown mechanism.
Ji A Sohn, Samuel Litwin, Christoph Seeger   +2 more
doaj   +1 more source

Inhibitors of the DNA repair enzyme AAG

, 2022
DNA, the genetic blueprint of life, is targeted by a multitude of damaging species of both internal and external origin. This damage can result in the loss of genomic integrity and increase susceptibility to immunodeficiency, neurological disorders, and cancers.
Hiles, Jacob D., Elliott, Ruan M., Meira, Lisiane B., Whelligan, Daniel K.   +3 more
openaire   +2 more sources

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

SIRT1 Enhances the Survival of Human Embryonic Stem Cells by Promoting DNA Repair

Stem Cell Reports, 2017
Summary: Human embryonic stem cells (hESCs) hold great promise for the treatment of many incurable diseases. Sirtuin1 (SIRT1), a class III histone deacetylase, is abundantly expressed in hESCs and is known to regulate early differentiation and telomere ...
Jiho Jang, Yong Jun Huh, Hyun-Ju Cho, Boram Lee, Jaepil Park, Dong-Youn Hwang, Dong-Wook Kim   +6 more
doaj   +1 more source