Results 81 to 90 of about 376,771 (302)

Exploiting metabolic adaptations to overcome dabrafenib treatment resistance in melanoma cells

Molecular Oncology, EarlyView.
We show that dabrafenib‐resistant melanoma cells undergo mitochondrial remodeling, leading to elevated respiration and ROS production balanced by stronger antioxidant defenses. This altered redox state promotes survival despite mitochondrial damage but renders resistant cells highly vulnerable to ROS‐inducing compounds such as PEITC, highlighting redox
Silvia Eller, Susanne Ebner, Carmen Haselrieder, Julia K. Günther, Astrid Drasche, Sophie Strich, Chiara Volani, Andrea Medici, Aleksandar Nikolajevic, Alex Deltedesco, Johannes E. Sigmund, Michael J. Blumer, Martin Hermann, Johanna Vanacker, Gerald Brandacher, Eduard Stefan, Omar Torres‐Quesada, Jakob Troppmair   +17 more
wiley   +1 more source

Analysis of the machinery and intermediates of the 5hmC-mediated DNA demethylation pathway in aging on samples from the MARKAGE Study [PDF]

, 2016
Gradual changes in the DNA methylation landscape occur throughout aging virtually in all human tissues. A widespread reduction of 5-methylcytosine (5mC), associated with highly reproducible site-specific hypermethylation, characterizes the genome in ...
Bacalini, Maria Giulia, Bernhardt, Jürgen, Breusing, Nicolle, Bürkle, Alexander, Caiafa, Paola, Calabrese, Roberta, Ciccarone, Fabio, Dollé, Martijn E.T., Franceschi, Claudio, Gonos, Efstathios S, Grune, Tilman, Grune, Tilman, Guastafierro, Tiziana, Hervonen, Antti, Jansen, Eugène, Koller, Bernhard, Malavolta, Marco, Moreno-Villanueva, María, Reale, Anna, Sikora, Ewa, Sindlinger, Thilo, Slagboom, P. Eline, Toussaint, Olivier, Valentini, Elisabetta, Zampieri, Michele   +24 more
core   +2 more sources

Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma

Molecular Oncology, EarlyView.
Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken, Andrea Abel Gutierrez, Imke van Rossum, Cornelia G. Spruijt, Michiel Vermeulen, Guido van Mierlo, Blanca Scheijen, Annemiek B. van Spriel   +7 more
wiley   +1 more source

PARP inhibition and pharmacological ascorbate demonstrate synergy in castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
Pharmacologic ascorbate (vitamin C) increases ROS, disrupts cellular metabolism, and induces DNA damage in CRPC cells. These effects sensitize tumors to PARP inhibition, producing synergistic growth suppression with olaparib in vitro and significantly delayed tumor progression in vivo. Pyruvate rescue confirms ROS‐dependent activity.
Nicolas Gordon, Peter T. Gallagher, Orly I. Richter, Neermala Poudel Neupane, Amy C. Mandigo, Jennifer J. McCann, Emanuela Dylgjeri, Irina Vasilevskaya, Christopher McNair, Channing J. Paller, Wm. Kevin Kelly, Karen E. Knudsen, Matthew J. Schiewer, Ayesha A. Shafi   +13 more
wiley   +1 more source

An archaeal family-B DNA polymerase variant able to replicate past DNA damage: occurrence of replicative and translesion synthesis polymerases within the B family [PDF]

, 2014
A mutant of the high fidelity family-B DNA polymerase from the archaeon Thermococcus gorgonarius (Tgo-Pol), able to replicate past DNA lesions, is described.
Aidan J. Doherty, Barry, Bebenek, Bernard A. Connolly, Biles, Boiteux, Braithwaite, Brautigam, Brian J. Keith, Burgers, Cadet, Cann, Cann, Cline, Cozens, Cubonová, Fenton, Firbank, Franklin, Gan, Goodman, Haracska, Haracska, Heller, Hogg, Ishino, Johnson, Johnson, Johnson, Joyce, Jozwiakowski, Jozwiakowski, Keith, Kelley, Kelman, Kim, Kunkel, Kunkel, Lawrence, Lawrence, Lawrence, Leadon, Leigh, Louise Gilroy, Lundberg, McCulloch, Nick McElhinny, Northam, Perera, Pettersen, Pfeifer, Prakash, Pursell, Rangarajan, Reha-Krantz, Sabouri, Sabouri, Sarmiento, Schneider, Shaw, Stanislaw K. Jozwiakowski, Stillman, Swan, Teoule, Terpe, Wang, Wang, Waters, Yang   +68 more
core   +1 more source

Peroxidasin enables melanoma immune escape by inhibiting natural killer cell cytotoxicity

Molecular Oncology, EarlyView.
Peroxidasin (PXDN) is secreted by melanoma cells and binds the NK cell receptor NKG2D, thereby suppressing NK cell activation and cytotoxicity. PXDN depletion restores NKG2D signaling and enables effective NK cell–mediated melanoma killing. These findings identify PXDN as a previously unrecognized immune evasion factor and a potential target to improve
Hsu‐Min Sung, David Bickel, Lena C. M. Krause, Daria Ezeriņa, Christian Ickes, Julian Wojtachnia, Christine S. Gibhardt, Magdalena Shumanska, Khadija Wahni, Andrea Paluschkiwitz, Julia Malo Pueyo, Ekaterina Baranova, Wim Vranken, Hedwig Stanisz, Ioana Stejerean‐Todoran, Michael P. Schön, Joris Messens, Ivan Bogeski   +17 more
wiley   +1 more source

The role of HECT-type E3 ubiquitin ligases in DNA damage response and repair

Cell Death Discovery
The post-translational modification ubiquitination consists in a three-step reaction triggered by E1 ubiquitin activating enzymes, E2 ubiquitin conjugating enzymes, and E3 ubiquitin ligases.
Sara Giovannini, Claudia Fiorilli, Valeria Moriconi, Yufang Shi, Eleonora Candi, Gerry Melino, Francesca Bernassola   +6 more
doaj   +1 more source

Early Steps in the DNA Base Excision Repair Pathway of a Fission Yeast Schizosaccharomyces pombe

Journal of Nucleic Acids, 2010
DNA base excision repair (BER) accounts for maintaining genomic integrity by removing damaged bases that are generated endogenously or induced by genotoxic agents.
Kyoichiro Kanamitsu, Shogo Ikeda
doaj   +1 more source

Altering DNA Repair to Improve Radiation Therapy: Specific and Multiple Pathway Targeting

Frontiers in Oncology, 2019
Radiation therapy (RT) is widely used in cancer care strategies. Its effectiveness relies mainly on its ability to cause lethal damage to the DNA of cancer cells.
Julian Biau, Julian Biau, Julian Biau, Julian Biau, Julian Biau, Julian Biau, Emmanuel Chautard, Emmanuel Chautard, Pierre Verrelle, Pierre Verrelle, Pierre Verrelle, Pierre Verrelle, Marie Dutreix, Marie Dutreix, Marie Dutreix, Marie Dutreix   +15 more
doaj   +1 more source

Predictors of hepatitis B cure using gene therapy to deliver DNA cleavage enzymes: a mathematical modeling approach. [PDF]

PLoS Computational Biology, 2013
Most chronic viral infections are managed with small molecule therapies that inhibit replication but are not curative because non-replicating viral forms can persist despite decades of suppressive treatment.
Joshua T Schiffer, Dave A Swan, Daniel Stone, Keith R Jerome   +3 more
doaj   +1 more source