Results 101 to 110 of about 397,727 (310)

RFLP-kenzy: a new bioinformatics tool for in silico detection of key restriction enzyme in RFLP technique

Beni-Suef University Journal of Basic and Applied Sciences
Background Today, several bioinformatics tools are available for analyzing restriction fragment length data. RFLP-kenzy is a new bioinformatic tool for identifying restriction key enzyme that cut at least 1 sequence and a maximum of n-1 sequence. Results
Nora Laref, Khadidja Belkheir, Mohamed Belazreg, Abdelhadi Hireche   +3 more
doaj   +1 more source

Terminal Restriction Fragments (TRF) Method to Analyze Telomere Lengths

Bio-Protocol, 2015
Chromosome ends - telomeres - are a focus of intensive research due to their importance for the maintenance of chromosome stability. Their shortening due to incomplete replication functions as a molecular clock counting the number of cell divisions, and ...
Miloslava Fojtová, Petr Fajkus, Pavla Polanská, Jiří Fajkus   +3 more
doaj   +1 more source

A species-specific satellite DNA family in the genome of the coffee root-knot nematode Meloidogyne exigua : Application to molecular diagnostics of the parasite [PDF]

, 2002
A new Bg/II satellite DNA has been isolated, cloned and sequenced from the coffee root-knot nematode, Meloidogyne exigua (Nematoda: Tylenchida). It is represented as tandemly repeated sequences with a monomeric unit of 277 bp. The monomers are present at
Bongiovanni, Michel, Carneiro, Regina Maria Dechechi Gomes, Castagnone Sereno, Philippe, Randig, Onivaldo, Sarah, Jean-Louis   +4 more
core   +2 more sources

Effective therapeutic targeting of CTNNB1‐mutant hepatoblastoma with WNTinib

Molecular Oncology, EarlyView.
WNTinib, a Wnt/CTNNB1 inhibitor, was tested in hepatoblastoma (HB) experimental models. It delayed tumor growth and improved survival in CTNNB1‐mutant in vivo models. In organoids, WNTinib outperformed cisplatin and showed enhanced efficacy in combination therapy, supporting its potential as a targeted treatment for CTNNB1‐mutated HB.
Ugne Balaseviciute, Júlia Huguet‐Pradell, Jordi Abril‐Fornaguera, Albert Gris‐Oliver, Alex Rialdi, Elisa Fernández‐Martínez, Carla Montironi, Vanessa Del Pozo, Peter Houghton, Laura Zanatto, Agavni Mesropian, Ieva Keraite, Swan Thung, Carolina Armengol, Pau Sancho‐Bru, Ernesto Guccione, Roser Pinyol, Josep M. Llovet   +17 more
wiley   +1 more source

Testing the usage of ITS2 and 28S-D2 markers in identifying some species of Ammophila, Prionyx, and Sphex genera (Hymenoptera: Sphecidae)

Journal of New Results in Science, 2015
In addition to traditional methods, investigation of rDNA gene regions with molecular techniques is widely used to identify insect taxa. In this study, the usability of the primers (TrITS2, 28S-D2) belonging to second expansion segment (D2) of the 28S ...
Ilyas Can, Yasar Gulmez
doaj  

Exploiting metabolic adaptations to overcome dabrafenib treatment resistance in melanoma cells

Molecular Oncology, EarlyView.
We show that dabrafenib‐resistant melanoma cells undergo mitochondrial remodeling, leading to elevated respiration and ROS production balanced by stronger antioxidant defenses. This altered redox state promotes survival despite mitochondrial damage but renders resistant cells highly vulnerable to ROS‐inducing compounds such as PEITC, highlighting redox
Silvia Eller, Susanne Ebner, Carmen Haselrieder, Julia K. Günther, Astrid Drasche, Sophie Strich, Chiara Volani, Andrea Medici, Aleksandar Nikolajevic, Alex Deltedesco, Johannes E. Sigmund, Michael J. Blumer, Martin Hermann, Johanna Vanacker, Gerald Brandacher, Eduard Stefan, Omar Torres‐Quesada, Jakob Troppmair   +17 more
wiley   +1 more source

Isolasi karakterisasi dan uji ekspresi gen ALP1 SI Escherichia coli DH5a

Journal of Biological Researches, 1999
Amylase gene (ALP1) insertion in YCp 50 cloning vector was detected by Direct Screening PCR. Result of Direct Screening PCR showed one transformants which assumed have amylase gene insert.
Ni Nyoman Tri Puspaningsih, Akhmaloka Akhmaloka, Sofyan Hadi, Y. Sriwulan Manuhara, Bambang Irawan   +4 more
doaj   +1 more source

Molecular characterization of Salmonella Enteritidis : comparison of an optimized multi-locus variable-number of tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis [PDF]

, 2012
Salmonella Enteritidis (SE) is a genetically homogenous serovar, which makes optimal subtype discrimination crucial for epidemiological research. This study describes the development and evaluation of an optimized multiple-locus variable number tandem ...
Bertrand, Sophie, De Reu, Koen, Dewaele, Isabelle, Ducatelle, Richard, Herman, Lieve, Heyndrickx, Marc, Imberechts, Hein, Rasschaert, Geertrui, Wattiau, Pierre, Wildemauwe, Christa   +9 more
core   +2 more sources

ATF4‐mediated stress response as a therapeutic vulnerability in chordoma

Molecular Oncology, EarlyView.
We screened 5 chordoma cell lines against 100+ inhibitors of epigenetic and metabolic pathways and kinases and identified halofuginone, a tRNA synthetase inhibitor. Mechanistically halofuginone induces an integrated stress response, with eIF2alpha phosphorylation, activation of ATF4 and its target genes CHOP, ASNS, INHBE leading to cell death ...
Lucia Cottone, James Dunford, Eleanor Calcutt, Vicki Gamble, Filiz Senbabaoglu Aksu, Lorena Ligammari, Georgina Wherry, Giorgia Gaeta, John C. Christianson, Adrienne M. Flanagan, Udo Oppermann, Adam P. Cribbs   +11 more
wiley   +1 more source

Functional Traits Co-Occurring with Mobile Genetic Elements in the Microbiome of the Atacama Desert [PDF]

, 2019
Mobile genetic elements (MGEs) play an essential role in bacterial adaptation and evolution. These elements are enriched within bacterial communities from extreme environments. However, very little is known if specific genes co-occur with MGEs in extreme
Airo, Alessandro, Schloter, Michael, Schulze-Makuch, Dirk, Sáenz, Johan S., Vestergaard, Gisle   +4 more
core   +1 more source