Results 11 to 20 of about 391,854 (357)
The average spacing of restriction enzyme recognition sites in DNA [PDF]
Journal of Theoretical Biology, 1982 The discovery of naturally occurring enzymes which cleave DNA at sites specific to particular nucleotide sequences has had a great impact on molecular biology. The function of these enzymes in uivo is to protect bacterial cells from viral invasion by degradation of foreign DNA.
Moore, Gordon P., Moore, Arnold R.
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Site-specific DNA transesterification catalyzed by a restriction enzyme [PDF]
Proceedings of the National Academy of Sciences, 2007 Most restriction endonucleases use Mg 2+ to hydrolyze phosphodiester bonds at specific DNA sites. We show here that BfiI, a metal-independent restriction enzyme from the phospholipase D superfamily, catalyzes both DNA hydrolysis and transesterification reactions at its recognition site.
Sasnauskas, G +3 more
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REBASE—a database for DNA restriction and modification: enzymes, genes and genomes [PDF]
Nucleic Acids Research, 2009 Abstract REBASE is a comprehensive and extensively curated database of information about the components of restriction-modification (RM) systems. It is fully referenced and provides information about the recognition and cleavage sites for both restriction enzymes and DNA methyltransferases together with their commercial availability ...
Richard J Roberts +3 more
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A physical map of human Alu repeats cleavage by restriction endonucleases
BMC Genomics, 2008 Background Alu repetitive elements are the abundant sequences in human genome. Diversity of DNA sequences of these elements makes difficult the construction of theoretical patterns of Alu repeats cleavage by restriction endonucleases.
Chernukhin Valery A +3 more
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BMC Evolutionary Biology, 2011 Background The discovery of restriction endonucleases and modification DNA methyltransferases, key instruments of genetic engineering, opened a new era of molecular biology through development of the recombinant DNA technology.
Solonin Alexander S +2 more
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BioTechniques, 1999 Microsatellite loci are highly informative genetic markers useful for population genetic studies, linkage mapping and parentage determination. Methods to identify novel microsatellite loci commonly use subtractive hybridization to enrich smallinsert ...
Matthew B. Hamilton +3 more
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Restriction Fragment Length Polymorphism Linkage Map for Arabidopsis thaliana [PDF]
, 1988 We have constructed a restriction fragment length polymorphism linkage map for the nuclear genome of the flowering plant Arabidopsis thaliana. The map, containing 90 randomly distributed molecular markers, is physically very dense; >50% of the genome is ...
Bowman, John L. +4 more
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Mechanism of DNA Recognition by the Restriction Enzyme EcoRV
Journal of Molecular Biology, 2010 EcoRV, a restriction enzyme in Escherichia coli, destroys invading foreign DNA by cleaving it at the center step of a GATATC sequence. In the EcoRV-cognate DNA crystallographic complex, a sharp kink of 50 degrees has been found at the center base-pair step (TA).
Zahran M +3 more
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Biomolecular computers with multiple restriction enzymes
Genetics and Molecular Biology, 2017 The development of conventional, silicon-based computers has several limitations, including some related to the Heisenberg uncertainty principle and the von Neumann “bottleneck”.
Sebastian Sakowski +5 more
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Pathological phenotypes and in vivo DNA cleavage by unrestrained activity of a phosphorothioate-based restriction system in Salmonella [PDF]
, 2014 Prokaryotes protect their genomes from foreign DNA with a diversity of defence mechanisms, including a widespread restriction–modification (R–M) system involving phosphorothioate (PT) modification of the DNA backbone. Unlike classical R–M systems, highly
Cao, Bo +8 more
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