Results 11 to 20 of about 390,387 (330)
Restriction enzyme digestion of hemimethylated DNA [PDF]
Nucleic Acids Research, 1981 Hemimethylated duplex DNA of the bacteriophage phi X 174 was synthesized using primed repair synthesis is in vitro with E. coli DNA polymerase I followed by ligation to produce the covalently closed circular duplex (RFI). Single-stranded phi X DNA was used as a template, a synthetic oligonucleotide as primer and 5-methyldeoxycytidine-5'-triphosphate ...
Howard Cedar +2 more
openaire +3 more sources
NEBcutter: a program to cleave DNA with restriction enzymes [PDF]
Nucleic Acids Research, 2003 NEBcutter, version 1.0, is a program available via a web server (http://tools.neb.com/NEBcutter) that will accept an input DNA sequence and produce a comprehensive report of the restriction enzymes that will cleave the sequence. It produces a variety of outputs including restriction enzyme maps, theoretical digests and links into the restriction enzyme
Richard J. Roberts +2 more
openaire +2 more sources
REBASE--enzymes and genes for DNA restriction and modification [PDF]
Nucleic Acids Research, 2007 REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in the biological process of restriction-modification. It contains fully referenced information about recognition and cleavage sites, isoschizomers, neoschizomers, commercial availability, methylation sensitivity, crystal ...
Richard J. Roberts +3 more
openaire +3 more sources
Use Cutting Enzymes to Encode the Secret Message [PDF]
Al-Rafidain Journal of Computer Sciences and Mathematics, 2014 Researchers begin to explore new methods to manage the huge amount of data shared over the Internet, in order to use them more efficiently and safely, One of the ways that is raised nowadays is Steganography, The art of information hiding within ...
Saadoun A.
doaj +1 more source
In vitro Type II Restriction of Bacteriophage DNA With Modified Pyrimidines
Frontiers in Microbiology, 2020 To counteract host-encoded restriction systems, bacteriophages (phages) incorporate modified bases in their genomes. For example, phages carry in their genomes modified pyrimidines such as 5-hydroxymethyl-cytosine (5hmC) in T4gt deficient in α- and β ...
Kiersten Flodman +4 more
doaj +1 more source
Revised selection criteria for candidate restriction enzymes in genome walking. [PDF]
PLoS ONE, 2012 A new method to improve the efficiency of flanking sequence identification by genome walking was developed based on an expanded, sequential list of criteria for selecting candidate enzymes, plus several other optimization steps.
Ali Taheri +3 more
doaj +1 more source
Fragmentation of bacteriophage S13 replicative from DNA by restriction endonucleases from Hemophilus influenzae and Hemophilus aegyptius. [PDF]
, 1976 The restriction enzymes Hind from Hemophilus influenzae and HaeIII from Hemophilus aegyptius cleave bacteriophage S13 replicative form (RF) DNA into 13 and 10 specific fragments, respectively.
Grosveld, F.G. (Frank) +2 more
core +9 more sources
Alleviation of restriction by DNA condensation and non-specific DNA binding ligands [PDF]
, 2004 During conditions of cell stress, the type I restriction and modification enzymes of bacteria show reduced, but not zero, levels of restriction of unmethylated foreign DNA.
Dryden, D T F, Keatch, S A, Su, T J
core +2 more sources
Restriction-modification system with methyl-inhibited base excision and abasic-site cleavage activities [PDF]
, 2015 The restriction-modification systems use epigenetic modification to distinguish between self and nonself DNA. A modification enzyme transfers a methyl group to a base in a specific DNA sequence while its cognate restriction enzyme introduces breaks in ...
Fukuyo, Masaki +9 more
core +5 more sources
BioTechniques, 1999 Microsatellite loci are highly informative genetic markers useful for population genetic studies, linkage mapping and parentage determination. Methods to identify novel microsatellite loci commonly use subtractive hybridization to enrich smallinsert ...
Matthew B. Hamilton +3 more
doaj +1 more source