Results 21 to 30 of about 390,387 (330)
Mechanism of DNA Recognition by the Restriction Enzyme EcoRV
Journal of Molecular Biology, 2010 EcoRV, a restriction enzyme in Escherichia coli, destroys invading foreign DNA by cleaving it at the center step of a GATATC sequence. In the EcoRV-cognate DNA crystallographic complex, a sharp kink of 50 degrees has been found at the center base-pair step (TA).
Zahran M +3 more
openaire +5 more sources
Biomolecular computers with multiple restriction enzymes
Genetics and Molecular Biology, 2017 The development of conventional, silicon-based computers has several limitations, including some related to the Heisenberg uncertainty principle and the von Neumann “bottleneck”.
Sebastian Sakowski +5 more
doaj +1 more source
Restriction Fragment Length Polymorphism Linkage Map for Arabidopsis thaliana [PDF]
, 1988 We have constructed a restriction fragment length polymorphism linkage map for the nuclear genome of the flowering plant Arabidopsis thaliana. The map, containing 90 randomly distributed molecular markers, is physically very dense; >50% of the genome is ...
Bowman, John L. +4 more
core +1 more source
Pathological phenotypes and in vivo DNA cleavage by unrestrained activity of a phosphorothioate-based restriction system in Salmonella [PDF]
, 2014 Prokaryotes protect their genomes from foreign DNA with a diversity of defence mechanisms, including a widespread restriction–modification (R–M) system involving phosphorothioate (PT) modification of the DNA backbone. Unlike classical R–M systems, highly
Cao, Bo +8 more
core +1 more source
An efficient method for DNA extraction from Cladosporioid fungi [PDF]
, 2010 We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A. +3 more
core +2 more sources
BioTechniques, 2001 A method for generating limited representations of total bacterial DNA, without prior knowledge of the DNA sequence, has been developed. This method consists of three steps: digestion with two restriction enzymes, ligation of two oligonucleotide adapters
Aleksander Masny, Andrzej Plucienniczak
doaj +1 more source
Enhancement of PCRs by Partial Restriction Digestion of Genomic Templates
BioTechniques, 1999 Genomic DNA preparations derived from mammalian cells can often exhibit poor template activity in PCR, particularly when carried out on target sequences present at low copy number. Using genomic DNA bearing SV40 sequences integrated into host chromosomal
Raymond Vázquez, Mark L. Steinberg
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Human tRNAGlu genes: their copy number and organisation [PDF]
, 1990 The tRNAGlu gene copy number, determined by genomic blot analysis of human plaeental DNA, is approximately thirteen. These studies, using several probes and DNA digested with several restriction enzymes singly or in combination, show that most of these ...
Goddard, John P., Gonos, Efstathios S.
core +1 more source
Straightforward Procedure for Laboratory Production of DNA Ladder
Journal of Nucleic Acids, 2012 DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare a DNA ladder that consists of 10 fragments from 100 to 1000 bp.
Vo Thi Thuong Lan +5 more
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Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities. [PDF]
PLoS Biology, 2016 The creation of restriction enzymes with programmable DNA-binding and -cleavage specificities has long been a goal of modern biology. The recently discovered Type IIL MmeI family of restriction-and-modification (RM) enzymes that possess a shared target ...
Scott J Callahan +6 more
doaj +1 more source