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Evaluation of a Quantitative DNA Methylation Analysis Technique using Methylation-Sensitive/Dependent Restriction Enzymes and Real-Time PCR [PDF]
Epigenetics, 2006 DNA methylation in mammals has been shown to play many important roles in diverse biological phenomena. Several methods have been developed for the measurement of region-specific levels of DNA methylation.
Christopher C. Oakes +3 more
openalex +2 more sources
Using nucleolytic toxins as restriction enzymes enables new RNA applications. [PDF]
Nucleic Acids ResOver the past five decades, DNA restriction enzymes have revolutionized biotechnology. While these enzymes are widely used in DNA research and DNA engineering, the emerging field of RNA and mRNA therapeutics requires sequence-specific RNA ...
Rothweiler U +11 more
europepmc +3 more sources
Site-specific DNA transesterification catalyzed by a restriction enzyme [PDF]
Proceedings of the National Academy of Sciences, 2007 Most restriction endonucleases use Mg 2+ to hydrolyze phosphodiester bonds at specific DNA sites. We show here that BfiI, a metal-independent restriction enzyme from the phospholipase D superfamily, catalyzes both DNA hydrolysis and transesterification reactions at its recognition site.
Giedrius Sasnauskas +3 more
openalex +6 more sources
Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering
Fermentation, 2023 Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
doaj +1 more source
REBASE: a database for DNA restriction and modification: enzymes, genes and genomes
Nucleic Acids Res., 2022 REBASE is a comprehensive and extensively curated database of information about the components of restriction-modification (RM) systems. It is fully referenced and provides information about the recognition and cleavage sites for both restriction enzymes
R. Roberts +3 more
semanticscholar +1 more source
Restriction enzyme digestion of hemimethylated DNA [PDF]
Nucleic Acids Research, 1981 Hemimethylated duplex DNA of the bacteriophage phi X 174 was synthesized using primed repair synthesis is in vitro with E. coli DNA polymerase I followed by ligation to produce the covalently closed circular duplex (RFI). Single-stranded phi X DNA was used as a template, a synthetic oligonucleotide as primer and 5-methyldeoxycytidine-5'-triphosphate ...
Howard Cedar +2 more
openaire +3 more sources
Use Cutting Enzymes to Encode the Secret Message [PDF]
Al-Rafidain Journal of Computer Sciences and Mathematics, 2014 Researchers begin to explore new methods to manage the huge amount of data shared over the Internet, in order to use them more efficiently and safely, One of the ways that is raised nowadays is Steganography, The art of information hiding within ...
Saadoun A.
doaj +1 more source
In vitro Type II Restriction of Bacteriophage DNA With Modified Pyrimidines
Frontiers in Microbiology, 2020 To counteract host-encoded restriction systems, bacteriophages (phages) incorporate modified bases in their genomes. For example, phages carry in their genomes modified pyrimidines such as 5-hydroxymethyl-cytosine (5hmC) in T4gt deficient in α- and β ...
Kiersten Flodman +4 more
doaj +1 more source
Topological digestion drives time-varying rheology of entangled DNA fluids [PDF]
, 2021 Understanding and controlling the rheology of polymeric complex fluids that are pushed out-of-equilibrium is a fundamental problem in both industry and biology. For example, to package, repair, and replicate DNA, cells use enzymes to constantly manipulate DNA topology, length, and structure.
arxiv +1 more source
Type I restriction endonucleases are true catalytic enzymes [PDF]
, 2009 Type I restriction endonucleases are intriguing, multifunctional complexes that restrict DNA randomly, at sites distant from the target sequence. Restriction at distant sites is facilitated by ATP hydrolysis-dependent, translocation of double-stranded ...
Berge +42 more
core +2 more sources