Results 21 to 30 of about 634,910 (347)
An efficient method for DNA extraction from Cladosporioid fungi [PDF]
, 2010 We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A. +3 more
core +2 more sources
Internuclear gene silencing in Phytophthora infestans is established through chromatin remodelling [PDF]
, 2008 In the plant pathogen Phytophthora infestans, nuclear integration of inf1 transgenic DNA sequences results in internuclear gene silencing of inf1. Although silencing is regulated at the transcriptional level, it also affects transcription from other ...
Appiah, A.A. +7 more
core +7 more sources
Alleviation of restriction by DNA condensation and non-specific DNA binding ligands [PDF]
, 2004 During conditions of cell stress, the type I restriction and modification enzymes of bacteria show reduced, but not zero, levels of restriction of unmethylated foreign DNA.
Dryden, D T F, Keatch, S A, Su, T J
core +3 more sources
Open Biology, 2015 Characterization of dynamic DNA methylomes in diverse phylogenetic groups has attracted growing interest for a better understanding of the evolution of DNA methylation as well as its function and biological significance in eukaryotes.
Shi Wang +10 more
semanticscholar +1 more source
Restriction enzyme mapping of vaccinia virus DNA [PDF]
Journal of Virology, 1982 The cleavage sites for the restriction enzymes Bg/I, HindIII, KpnI, SalI, SmaI, and XhoI were located, from primary data, on the DNA isolated from the WR strain of vaccinia virus. Bg/I and SmaI divide the DNA into five segments which can be isolated by sucrose gradient centrifugation.
openaire +2 more sources
BioTechniques, 2001 A method for generating limited representations of total bacterial DNA, without prior knowledge of the DNA sequence, has been developed. This method consists of three steps: digestion with two restriction enzymes, ligation of two oligonucleotide adapters
Aleksander Masny, Andrzej Plucienniczak
doaj +1 more source
Enhancement of PCRs by Partial Restriction Digestion of Genomic Templates
BioTechniques, 1999 Genomic DNA preparations derived from mammalian cells can often exhibit poor template activity in PCR, particularly when carried out on target sequences present at low copy number. Using genomic DNA bearing SV40 sequences integrated into host chromosomal
Raymond Vázquez, Mark L. Steinberg
doaj +1 more source
Re-evaluating the kinetics of ATP hydrolysis during initiation of DNA sliding by Type III restriction enzymes [PDF]
, 2015 DNA cleavage by the Type III restriction enzymes requires long-range protein communication between recognition sites facilitated by thermally-driven 1D diffusion. This ‘DNA sliding’ is initiated by hydrolysis of multiple ATPs catalysed by a helicase-like
Bollins, Jack M +2 more
core +3 more sources
REBASE—a database for DNA restriction and modification: enzymes, genes and genomes
Nucleic Acids Res., 2009 REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in the biological process of restriction–modification (R–M).
R. Roberts +3 more
semanticscholar +1 more source
Straightforward Procedure for Laboratory Production of DNA Ladder
Journal of Nucleic Acids, 2012 DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare a DNA ladder that consists of 10 fragments from 100 to 1000 bp.
Vo Thi Thuong Lan +5 more
doaj +1 more source