Results 21 to 30 of about 391,854 (357)
An efficient method for DNA extraction from Cladosporioid fungi [PDF]
, 2010 We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A. +3 more
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BioTechniques, 2001 A method for generating limited representations of total bacterial DNA, without prior knowledge of the DNA sequence, has been developed. This method consists of three steps: digestion with two restriction enzymes, ligation of two oligonucleotide adapters
Aleksander Masny, Andrzej Plucienniczak
doaj +1 more source
Alleviation of restriction by DNA condensation and non-specific DNA binding ligands [PDF]
, 2004 During conditions of cell stress, the type I restriction and modification enzymes of bacteria show reduced, but not zero, levels of restriction of unmethylated foreign DNA.
Dryden, D T F, Keatch, S A, Su, T J
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On the DNA cleavage mechanism of Type I restriction enzymes [PDF]
Nucleic Acids Research, 2005 Although the DNA cleavage mechanism of Type I restriction-modification enzymes has been extensively studied, the mode of cleavage remains elusive. In this work, DNA ends produced by EcoKI, EcoAI and EcoR124I, members of the Type IA, IB and IC families, respectively, have been characterized by cloning and sequencing restriction products from the ...
Jindrova, Eva +4 more
openaire +5 more sources
Enhancement of PCRs by Partial Restriction Digestion of Genomic Templates
BioTechniques, 1999 Genomic DNA preparations derived from mammalian cells can often exhibit poor template activity in PCR, particularly when carried out on target sequences present at low copy number. Using genomic DNA bearing SV40 sequences integrated into host chromosomal
Raymond Vázquez, Mark L. Steinberg
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Re-evaluating the kinetics of ATP hydrolysis during initiation of DNA sliding by Type III restriction enzymes [PDF]
, 2015 DNA cleavage by the Type III restriction enzymes requires long-range protein communication between recognition sites facilitated by thermally-driven 1D diffusion. This ‘DNA sliding’ is initiated by hydrolysis of multiple ATPs catalysed by a helicase-like
Bollins, Jack M +2 more
core +2 more sources
Straightforward Procedure for Laboratory Production of DNA Ladder
Journal of Nucleic Acids, 2012 DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare a DNA ladder that consists of 10 fragments from 100 to 1000 bp.
Vo Thi Thuong Lan +5 more
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Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities. [PDF]
PLoS Biology, 2016 The creation of restriction enzymes with programmable DNA-binding and -cleavage specificities has long been a goal of modern biology. The recently discovered Type IIL MmeI family of restriction-and-modification (RM) enzymes that possess a shared target ...
Scott J Callahan +6 more
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Suppression of APOBEC3-mediated restriction of HIV-1 by Vif
Frontiers in Microbiology, 2014 The APOBEC3 restriction factors are a family of deoxycytidine deaminases that are able to suppress replication of viruses with a single-stranded DNA intermediate by inducing mutagenesis and functional inactivation of the virus.
Yuqing eFeng +3 more
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Frontiers in Microbiology, 2021 Thermococcus kodakarensis (T. kodakarensis), a hyperthermophilic, genetically accessible model archaeon, encodes two putative restriction modification (R-M) defense systems, TkoI and TkoII.
Kelly M. Zatopek +6 more
doaj +1 more source