Results 41 to 50 of about 405,469 (354)

An Efficient PCR-RFLP Method for the Rapid Identification of Korean Pyropia Species

Molecules, 2017
The present study utilizes polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using partial plastid rbcL and mitochondrial trnC–trnP gene sequences to distinguish the six representative Pyropia species produced via ...
Yonguk Kim, Sung-Je Choi, Chulyung Choi
doaj   +1 more source

Machines on Genes: Enzymes that Make, Break and Move DNA and RNA [PDF]

, 2010
As the vital information repositories of the cell, the nucleic acids DNA and RNA pose many challenges as enzyme substrates. To produce, maintain and repair DNA and RNA, and to extract the genetic information that they encode, a battery of remarkable ...
Bates, Belousoff, Ben F. Luisi, Lilley, Marshall, Olorunniji, Rice, Richard P. Bowater, Sherratt, Smith, Szczelkun, Tomlinson, W. Marshall Stark, Weinzierl   +13 more
core   +1 more source

Cytosine-to-Uracil Deamination by SssI DNA Methyltransferase [PDF]

, 2013
The prokaryotic DNA(cytosine-5)methyltransferase M.SssI shares the specificity of eukaryotic DNA methyltransferases (CG) and is an important model and experimental tool in the study of eukaryotic DNA methylation.
A Beletskii, A Jeltsch, AN Sharath, Antal Kiss, AS Yang, B Bandaru, B Bandaru, B Chaikind, BM Hall, BTF van der Gun, DM Woodcock, EA Raleigh, EU Selker, EV Koudan, G Pósfai, GL Xu, H Gowher, I Stier, Ildikó Stier, J Sambrook, JC Shen, JC Shen, JC Shen, JM Zingg, JM Zingg, KM Reinisch, LA Frederico, LM Guzman, M Frommer, M Wyszynski, Martin G. Marinus, MH Sibley, P Rathert, P Renbaum, PA Waite-Rees, PM Warnecke, R Métivier, R Shapiro, RJ Roberts, RY-H Wang, S Kumar, SE Luria, T Durfee, TA Kunkel, UT Sankpal, WA Rosche, X Cheng, X Zhang   +47 more
core   +8 more sources

EcoRV catalysis with a pre-bent substrate

AIP Advances, 2015
Enzymes are deformable molecules which often adapt their conformation to the substrate’s geometry. In the case of restriction enzymes acting on DNA, the substrate (DNA) is deformable also.
Daniel S. Sanchez, Giovanni Zocchi
doaj   +1 more source

APOBECs and Herpesviruses

Viruses, 2021
The apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (APOBEC) family of DNA cytosine deaminases provides a broad and overlapping defense against viral infections.
Adam Z. Cheng, Sofia N. Moraes, Nadine M. Shaban, Elisa Fanunza, Craig J. Bierle, Peter J. Southern, Wade A. Bresnahan, Stephen A. Rice, Reuben S. Harris   +8 more
doaj   +1 more source

Poly(ADP-ribosyl)ation is involved in the epigenetic control of TET1 gene transcription [PDF]

, 2014
TET enzymes are the epigenetic factors involved in the formation of the Sixth DNA base 5-hydroxymethylcytosine, whose deregulation has been associated with tumorigenesis.
Bacalini, MARIA GIULIA, Caiafa, Paola, Calabrese, Roberta, Ciccarone, Fabio, Franceschi, C, Guastafierro, Tiziana, Mariano, Germano, Reale, Anna, Valentini, Elisabetta, Zampieri, Michele   +9 more
core   +6 more sources

REHUNT: a reliable and open source package for restriction enzyme hunting

BMC Bioinformatics, 2018
Background Restriction enzymes are used frequently in biotechnology. However, manual mining of restriction enzymes is challenging. Furthermore, integrating available restriction enzymes into different bioinformatics systems is necessary for many ...
Yu-Huei Cheng, Jiun-Jian Liaw, Che-Nan Kuo   +2 more
doaj   +1 more source

Archaeal diversity in deep-sea sediments estimated by means of different Terminal-Restriction Fragment Length Polymorphisms (T-RFLP) protocols [PDF]

, 2009
Despite the increasing recognition of the quantitative importance of Archaea in all marine systems, the protocols for a rapid estimate of Archaeal diversity patterns in deep-sea sediments have been only poorly tested yet.
Danovaro, Roberto, Luna, Gian Marco, Pusceddu, Antonio, Stumm, Karen   +3 more
core   +1 more source

PCR-RFLP of mitochondrial DNA of swamp buffaloes in breeding station

Italian Journal of Animal Science, 2010
Methods have been devised for detecting polymorphisms in the D-loop regions using the PCR followed by restriction enzyme digestion to reveal restriction fragment length polymorphism.
M. Kamonpatana, S. Usawang, W. Nualchuen, K. Tasripoo, S. Chethasing, C. Pansin, K. Srisakwattana   +6 more
doaj   +1 more source

Whole genome phylogeny for 21 Drosophila species using predicted 2b-RAD fragments [PDF]

PeerJ, 2013
Type IIB restriction endonucleases are site-specific endonucleases that cut both strands of double-stranded DNA upstream and downstream of their recognition sequences.
Arun S. Seetharam, Gary W. Stuart
doaj   +2 more sources