Results 41 to 50 of about 634,910 (347)

Microarray-Based Analysis of Methylation Status of CpGs in Placental DNA and Maternal Blood DNA--Potential New Epigenetic Biomarkers for Cell Free Fetal DNA-Based Diagnosis. [PDF]

PLoS ONE, 2015
Epigenetic markers for cell free fetal DNA in the maternal blood circulation are highly interesting in the field of non-invasive prenatal testing since such markers will offer a possibility to quantify the amount of fetal DNA derived from different ...
Lotte Hatt, Mads M Aagaard, Jesper Graakjaer, Cathrine Bach, Steffen Sommer, Inge E Agerholm, Steen Kølvraa, Anders Bojesen   +7 more
doaj   +1 more source

A comparison of DNA cleavage by the restriction enzymes SalPI and PstI [PDF]

Nucleic Acids Research, 1980
Methods for obtaining highly active, exonuclease-free, stable preparations of the Streptomyces albus P restriction enzyme SalPI are described. SalPI and its isoschizomer PstI (from the taxonomically distant Providencia stuartii 164) both cleave their recognition sequence (5'-CTGCAG-3') to generate fragments terminating in tetranucleotide 3' extensions ...
J A, Carter, K F, Chater, C J, Bruton, N L, Brown   +3 more
openaire   +2 more sources

An Efficient PCR-RFLP Method for the Rapid Identification of Korean Pyropia Species

Molecules, 2017
The present study utilizes polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using partial plastid rbcL and mitochondrial trnC–trnP gene sequences to distinguish the six representative Pyropia species produced via ...
Yonguk Kim, Sung-Je Choi, Chulyung Choi
doaj   +1 more source

Sirtuin functions and modulation: from chemistry to the clinic [PDF]

, 2016
Sirtuins are NAD+ -dependent histone deacetylases regulating important metabolic pathways in prokaryotes and eukaryotes and are involved in many biological processes such as cell survival, senescence, proliferation, apoptosis, DNA repair, cell ...
Altucci, Lucia, Carafa, Vincenzo, Cuomo, Francesca, Forgione, Mariantonietta, Hailu, GEBREMEDHIN SOLOMON, Jarho, Elina, Lahtela Kakkonen, Maija, Mai, Antonello, Rotili, Dante, Serretiello, Enrica   +9 more
core   +2 more sources

The average spacing of restriction enzyme recognition sites in DNA [PDF]

Journal of Theoretical Biology, 1982
The discovery of naturally occurring enzymes which cleave DNA at sites specific to particular nucleotide sequences has had a great impact on molecular biology. The function of these enzymes in uivo is to protect bacterial cells from viral invasion by degradation of foreign DNA.
Moore, Gordon P., Moore, Arnold R.
openaire   +3 more sources

EcoRV catalysis with a pre-bent substrate

AIP Advances, 2015
Enzymes are deformable molecules which often adapt their conformation to the substrate’s geometry. In the case of restriction enzymes acting on DNA, the substrate (DNA) is deformable also.
Daniel S. Sanchez, Giovanni Zocchi
doaj   +1 more source

Machines on Genes: Enzymes that Make, Break and Move DNA and RNA [PDF]

, 2010
As the vital information repositories of the cell, the nucleic acids DNA and RNA pose many challenges as enzyme substrates. To produce, maintain and repair DNA and RNA, and to extract the genetic information that they encode, a battery of remarkable ...
Bates, Belousoff, Ben F. Luisi, Lilley, Marshall, Olorunniji, Rice, Richard P. Bowater, Sherratt, Smith, Szczelkun, Tomlinson, W. Marshall Stark, Weinzierl   +13 more
core   +1 more source

Genome-wide DNA methylation profiling using the methylation-dependent restriction enzyme LpnPI

Genome Research, 2018
DNA methylation is a well-known epigenetic modification that plays a crucial role in gene regulation, but genome-wide analysis of DNA methylation remains technically challenging and costly.
R. Boers, J. Boers, B. de Hoon, C. Kockx, Zeliha Ozgur, A. Molijn, W. V. van Ijcken, J. Laven, J. Gribnau   +8 more
semanticscholar   +1 more source

Mechanism of DNA Recognition by the Restriction Enzyme EcoRV

Journal of Molecular Biology, 2010
EcoRV, a restriction enzyme in Escherichia coli, destroys invading foreign DNA by cleaving it at the center step of a GATATC sequence. In the EcoRV-cognate DNA crystallographic complex, a sharp kink of 50 degrees has been found at the center base-pair step (TA).
Zahran M, DAIDONE, ISABELLA, Smith JC, Imhof P.   +3 more
openaire   +4 more sources

On the DNA cleavage mechanism of Type I restriction enzymes [PDF]

Nucleic Acids Research, 2005
Although the DNA cleavage mechanism of Type I restriction-modification enzymes has been extensively studied, the mode of cleavage remains elusive. In this work, DNA ends produced by EcoKI, EcoAI and EcoR124I, members of the Type IA, IB and IC families, respectively, have been characterized by cloning and sequencing restriction products from the ...
Jindrova, Eva, Schmid-Nuoffer, Stefanie, Hamburger, Fabienne, Janscak, Pavel, Bickle, Thomas A.   +4 more
openaire   +4 more sources