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Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities. [PDF]
PLoS Biology, 2016 The creation of restriction enzymes with programmable DNA-binding and -cleavage specificities has long been a goal of modern biology. The recently discovered Type IIL MmeI family of restriction-and-modification (RM) enzymes that possess a shared target ...
Scott J Callahan +6 more
doaj +1 more source
The Helicase-Like Domains of Type III Restriction Enzymes Trigger Long-Range Diffusion Along DNA
Science, 2013 Sliding Restriction Helicase enzymes access the genetic information stored in double-helical DNA and RNA by opening the individual strands. Pseudo-helicases, including bacterial Type III restriction enzymes, use adenosine triphosphate (ATP) hydrolysis to
Friedrich W. Schwarz +6 more
semanticscholar +1 more source
Pathological phenotypes and in vivo DNA cleavage by unrestrained activity of a phosphorothioate-based restriction system in Salmonella [PDF]
, 2014 Prokaryotes protect their genomes from foreign DNA with a diversity of defence mechanisms, including a widespread restriction–modification (R–M) system involving phosphorothioate (PT) modification of the DNA backbone. Unlike classical R–M systems, highly
Cao, Bo +8 more
core +1 more source
PLoS ONE, 2012 Type I restriction-modification (RM) systems are comprised of two multi-subunit enzymes, the methyltransferase (∼160 kDa), responsible for methylation of DNA, and the restriction endonuclease (∼400 kDa), responsible for DNA cleavage. Both enzymes share a
James E Taylor +4 more
doaj +1 more source
Frontiers in Microbiology, 2021 Thermococcus kodakarensis (T. kodakarensis), a hyperthermophilic, genetically accessible model archaeon, encodes two putative restriction modification (R-M) defense systems, TkoI and TkoII.
Kelly M. Zatopek +6 more
doaj +1 more source
Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes
PLoS ONE, 2009 We have developed a protocol to assemble in one step and one tube at least nine separate DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained containing the desired construct.
Carola Engler +3 more
semanticscholar +1 more source
Restriction enzyme mapping of vaccinia virus DNA [PDF]
Journal of Virology, 1982 The cleavage sites for the restriction enzymes Bg/I, HindIII, KpnI, SalI, SmaI, and XhoI were located, from primary data, on the DNA isolated from the WR strain of vaccinia virus. Bg/I and SmaI divide the DNA into five segments which can be isolated by sucrose gradient centrifugation.
openaire +2 more sources
Suppression of APOBEC3-mediated restriction of HIV-1 by Vif
Frontiers in Microbiology, 2014 The APOBEC3 restriction factors are a family of deoxycytidine deaminases that are able to suppress replication of viruses with a single-stranded DNA intermediate by inducing mutagenesis and functional inactivation of the virus.
Yuqing eFeng +3 more
doaj +1 more source
Computation of splicing languages from DNA splicing system with one palindromic restriction enzyme [PDF]
, 2018 In DNA splicing system, the potential effects of sets of restriction enzymes and a ligase that allow DNA molecules to be cleaved and reassociated to produce further molecules are studied.
Fong, Wan Heng +2 more
core +1 more source
Charge transport-mediated recruitment of DNA repair enzymes [PDF]
Journal of Chemical Physics, 129, 235101, (2008), 2008 Damaged or mismatched bases in DNA can be repaired by Base Excision Repair (BER) enzymes that replace the defective base. Although the detailed molecular structures of many BER enzymes are known, how they colocalize to lesions remains unclear. One hypothesis involves charge transport (CT) along DNA [Yavin, {\it et al.}, PNAS, {\bf 102}, 3546, (2005 ...
arxiv +1 more source