Algorithms for optimizing cross-overs in DNA shuffling. [PDF]
BMC Bioinformatics, 2012 DNA shuffling generates combinatorial libraries of chimeric genes by stochastically recombining parent genes. The resulting libraries are subjected to large-scale genetic selection or screening to identify those chimeras with favorable properties (e.g ...
He L, Friedman AM, Bailey-Kellogg C.
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Characterization of Protein Domain Function via in vitro DNA Shuffling [PDF]
Bio-Protocol, 2018 We recently investigated the molecular events that drive evolution of the CTX-M-type β-lactamases by DNA shuffling of fragments of the blaCTX-M-14 and blaCTX-M-15 genes.
Kathy Po, Edward Chan, Sheng Chen
doaj +2 more sources
Directed DNA shuffling of retrovirus and retrotransposon integrase protein domains. [PDF]
PLoS ONE, 2013 Chimeric proteins are used to study protein domain functions and to recombine protein domains for novel or optimal functions. We used a library of chimeric integrase proteins to study DNA integration specificity.
Xiaojie Qi +6 more
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A novel chimeric avidin with increased thermal stability using DNA shuffling. [PDF]
PLoS ONE, 2014 Avidins are a family of proteins widely employed in biotechnology. We have previously shown that functional chimeric mutant proteins can be created from avidin and avidin-related protein 2 using a methodology combining random mutagenesis by recombination
Barbara Taskinen +6 more
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DNA shuffling to improve crude-water interfacial activity in biosurfactants with OmpA protein of Escherichia coli [PDF]
PeerJSurfactants are molecules derived primarily from petroleum that can reduce the surface tension at interfaces. Their slow degradation is a characteristic that could cause environmental issues.
Vanessa Lucía Nuñez Velez +8 more
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Toxic Activity, Molecular Modeling and Docking Simulations of Bacillus thuringiensis Cry11 Toxin Variants Obtained via DNA Shuffling [PDF]
Frontiers in Microbiology, 2018 The Cry11 family belongs to a large group of δ-endotoxins that share three distinct structural domains. Among the dipteran-active toxins referred to as three-domain Cry11 toxins, the Cry11Aa protein from Bacillus thuringiensis subsp.
Alvaro Mauricio Florez +8 more
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Expanding the Plant GSTome Through Directed Evolution: DNA Shuffling for the Generation of New Synthetic Enzymes With Engineered Catalytic and Binding Properties [PDF]
Frontiers in Plant Science, 2018 Glutathione transferases (GSTs, EC. 2.5.1.18) are inducible multifunctional enzymes that are essential in the detoxification and degradation of toxic compounds. GSTs have considerable biotechnological potential.
Evangelia G. Chronopoulou +5 more
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Enhanced Enzyme Performance by DNA Shuffling
CHIMIA, 1999 In the quest for enhanced enzyme performance in non-natural applications, directed evolution emulates natural processes for the generation of diversity and selection of desirable traits.
Andreas Crameri
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Enhanced catalytic efficiency of CotA-laccase by DNA shuffling. [PDF]
Bioengineered, 2019 Bacterial CotA-laccases exhibit higher activity in alkaline pH and salt concentration conditions compared to laccases from white-rot fungi. They are considered as green catalysts in decolorizing of industrial dyes. However, CotA-laccases are limited due to the low yield and catalytic efficiency as the spore-bound nature of CotA.
Ouyang F, Zhao M.
europepmc +4 more sources
Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes.
PLoS ONE, 2009 We have developed a protocol to assemble in one step and one tube at least nine separate DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained containing the desired construct.
Carola Engler +3 more
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