Stepwise error‐prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilicBacillus sp [PDF]
FEBS Open Bio, 2015 Cyclodextrin glucanotransferase (EC 2.4.1.19) from the alkaliphilicBacillus sp. G‐825‐6 converts starch mainly to γ‐cyclodextrin (CD8). A combination of error‐prone PCR and DNA shuffling was used to obtain variants of this enzyme with higher product ...
Susanne Melzer +3 more
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Shuffled: a software suite that assists the analysis of recombinant products resulting from DNA shuffling [PDF]
BioTechniques, 2004 DNA, Unshuffled In vitro recombination of gene variants for the purpose of generating libraries of novel chimeric genes is a powerful tool for the directed evolution of proteins.
Enrique Morett +1 more
doaj +3 more sources
Development and functional analysis of novel genetic promoters using DNA shuffling, hybridization and a combination thereof. [PDF]
PLoS ONE, 2012 BACKGROUND: Development of novel synthetic promoters with enhanced regulatory activity is of great value for a diverse range of plant biotechnology applications.
Rajiv Ranjan +5 more
doaj +2 more sources
Broadening the heterologous cross-neutralizing antibody inducing ability of porcine reproductive and respiratory syndrome virus by breeding the GP4 or M genes. [PDF]
PLoS ONE, 2013 Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important swine pathogens, which causes reproductive failure in sows and respiratory disease in piglets. A major hurdle to control PRRSV is the ineffectiveness of
Lei Zhou +8 more
doaj +1 more source
Predicting crossover generation in DNA shuffling [PDF]
Proceedings of the National Academy of Sciences, 2001 We introduce a quantitative framework for assessing the generation of crossovers in DNA shuffling experiments. The approach uses free energy calculations and complete sequence information to model the annealing process. Statistics obtained for the annealing events then are combined with a reassembly algorithm to infer crossover ...
G L, Moore +3 more
openaire +2 more sources
DNA shuffling brigthens prospects for GFP [PDF]
Nature Biotechnology, 1996 This article reviews a new technique emerging as an efficient way to direct protein evolution called `DNA shuffling`. This process uses the effective green fluorescent protein (GFP) as a control signal for certain proteins. 6 refs., 1 fig.
I, Matsumura, A D, Ellington
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DNA family shuffling of hyperthermostable β-glycosidases [PDF]
Biochemical Journal, 2002 The structural compatibility of two hyperthermostable family 1 glycoside hydrolases, Pyrococcus furiosus CelB and Sulfolobus solfataricus LacS, as well as their kinetic potential were studied by construction of a library of 2048 hybrid β-glycosidases using DNA family shuffling.
Kaper, T. +4 more
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Revealing biases inherent in recombination protocols
BMC Biotechnology, 2007 Background The recombination of homologous genes is an effective protein engineering tool to evolve proteins. DNA shuffling by gene fragmentation and reassembly has dominated the literature since its first publication, but this fragmentation-based method
Tang Xiao-Song +6 more
doaj +1 more source
DNA profiling of saliva traces habitually deposited on various documents: a pilot study
Egyptian Journal of Forensic Sciences, 2020 Background A study was conducted to determine whether the habitualness among individuals to apply saliva to their fingertips (for moistening) while shuffling through pages of a document can provide evidence in cases pertaining to handling of documents in
Mukesh Kumar Thakar +4 more
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Eukaryotic translation elongation factor 1A (eEF1A) domain I from S. cerevisiae is required but not sufficient for inter-species complementation [PDF]
, 2012 Ethanolamine phosphoglycerol (EPG) is a protein modification attached exclusively to eukaryotic elongation factor 1A (eEF1A). In mammals and plants, EPG is linked to conserved glutamate residues located in eEF1A domains II and III, whereas in the ...
Eva Greganova +5 more
core +9 more sources