Molecular Therapy: Methods & Clinical Development, 2019 Adeno-associated virus (AAV) vectors have become one of the most widely used gene transfer tools in human gene therapy. Considerable effort is currently being focused on AAV capsid engineering strategies with the aim of developing novel variants with ...
Marti Cabanes-Creus +15 more
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A Hierarchical Approach to Protein Molecular Evolution [PDF]
, 1999 Biological diversity has evolved despite the essentially infinite complexity of protein sequence space. We present a hierarchical approach to the efficient searching of this space and quantify the evolutionary potential of our approach with Monte Carlo ...
Crameri +30 more
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Attenuation of porcine reproductive and respiratory syndrome virus by molecular breeding of virus envelope genes from genetically divergent strains [PDF]
, 2013 Molecular breeding via DNA shuffling can direct the evolution of viruses with desired traits. By using a positive-strand RNA virus, porcine reproductive and respiratory syndrome virus (PRRSV), as a model, rapid attenuation of the virus was achieved in ...
An +52 more
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DNA Shuffling Method for Generating Estrogen Receptor α and β Chimeras in Yeast
BioTechniques, 2003 To facilitate our study of the molecular basis for the estrogen receptor (ER) subtype selectivity of novel ligands, we used DNA shuffling to construct chimeric ERs having ligand binding domains derived from both ERα and ERβ.
Jun Sun +3 more
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Cassette hybridization for vector assembly application in antibody chain shuffling
BioTechniques, 2018 Gene assembly methods are an integral part of molecular cloning experiments. The majority of existing vector assembly methods stipulate a need for exonucleases, endonucleases and/or the use of single-stranded DNA as starting materials.
Jing Yi Lai +3 more
doaj +1 more source
A single amino acid substitution in the alpha 3 domain of an H-2 class I molecule abrogates reactivity with CTL. [PDF]
, 1987 We previously described a somatic cell expressing a variant H-2Dd molecule that did not serve as a target for alloreactive anti-Dd CTL. The mutant cell line had been isolated by its failure to express a serological epitope present on the H-2Dd alpha 3 ...
Bluestone, JA, Potter, TA, Rajan, TV
core +2 more sources
Large-scale structural analysis of the core promoter in mammalian and plant genomes [PDF]
, 2005 DNA encodes at least two independent levels of functional information. The first level is for encoding proteins and sequence targets for DNA-binding factors, while the second one is contained in the physical and structural properties of the DNA molecule ...
Florquin, Kobe +4 more
core +1 more source
Development of a D-xylose fermenting and inhibitor tolerant industrial Saccharomyces cerevisiae strain with high performance in lignocellulose hydrolysates using metabolic and evolutionary engineering [PDF]
, 2013 Background: The production of bioethanol from lignocellulose hydrolysates requires a robust, D-xylose-fermenting and inhibitor-tolerant microorganism as catalyst.
Boles, Eckhard +13 more
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Phase and antigenic variation in mycoplasmas [PDF]
, 2010 With their reduced genome bound by a single membrane, bacteria of the Mycoplasma species represent some of the simplest autonomous life forms. Yet, these minute prokaryotes are able to establish persistent infection in a wide range of hosts, even in the ...
Baranowski, Eric +2 more
core +2 more sources
Applications of mutagenesis methods on affinity maturation of antibodies in vitro
浙江大学学报. 农业与生命科学版, 2016 Antibodies selected from naive antibody libraries usually have low affinity at the micromolar level. Meanwhile, the affinities of natural antibodies obtained by repeated immunization were also observed to have a limit around 100 picomole because of the ...
LIU Yuan +7 more
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