Results 41 to 50 of about 34,995 (285)

Correcting errors in synthetic DNA through consensus shuffling [PDF]

Nucleic Acids Research, 2005
Although efficient methods exist to assemble synthetic oligonucleotides into genes and genomes, these suffer from the presence of 1-3 random errors/kb of DNA. Here, we introduce a new method termed consensus shuffling and demonstrate its use to significantly reduce random errors in synthetic DNA.
Binkowski, Brock F., Richmond, Kathryn E., Kaysen, James, Sussman, Michael R., Belshaw, Peter J.   +4 more
openaire   +2 more sources

DNA shuffling as a tool for protein crystallization [PDF]

Proceedings of the National Academy of Sciences, 2005
The success of structural studies performed on an individual target in small scale or on many targets in the systemwide scale of structural genomics depends critically on three parameters: ( i ) obtaining an expression system capable of producing large quantities of the macromolecule(s) of ...
Robert J, Keenan, Daniel L, Siehl, Rebecca, Gorton, Linda A, Castle   +3 more
openaire   +2 more sources

Improving the Evaluation and Management of Transfusion‐Related Iron Overload in Children, Adolescents, and Young Adults Following Cancer Treatment or Hematopoietic Stem Cell Transplantation

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Transfusion‐related iron overload (TRIO) is a late effect of therapy impacting survivors of childhood cancer and hematopoietic stem cell transplantation (HSCT) who receive frequent packed red blood cell (pRBC) transfusions. Surprisingly, there are no accepted guidelines to assist providers in identifying and treating at‐risk ...
Luke Gingell, Allison Close, Guoli Zhou, Troy C. Quigg   +3 more
wiley   +1 more source

ReX: A suite of computational tools for the design, visualization, and analysis of chimeric protein libraries

BioTechniques, 2016
Directed evolution has greatly facilitated protein engineering and provided new insights into protein structure—function relationships. DNA shuffling using restriction enzymes is a particularly simple and cost-effective means of recombinatorial evolution
Weiliang Huang, Wayne A. Johnston, Mikael Boden, Elizabeth M.J. Gillam   +3 more
doaj   +1 more source

Shuffling of DNA Cassettes in a Synthetic Integron [PDF]

, 2013
The complexity of even small gene networks makes them hardly amenable to rational design. Testing random combinations of genetic elements in a directed evolution procedure is thus of interest for many applications including metabolic engineering. Here we describe how the recombination machinery of class 1 integrons can be used to deliver and shuffle ...
Bikard, David, Mazel, Didier
openaire   +3 more sources

Novel Genetic Risk Factor Identified for L‐Asparaginase‐Induced Pancreatitis in Pediatric Patients With Cancer

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background L‐asparaginase is a critical component in treatment protocols for pediatric acute lymphoblastic leukemia. Acute pancreatitis reactions can necessitate delays and, in some cases, discontinuation of L‐asparaginase, which compromises outcomes.
Edward J. Raack, Wan‐Chun Chang, Miguel Cordova‐Delgado, Spencer J. Anderson, Jessica N. Trueman, Erika N. Scott, Catrina M. Loucks, Shahrad R. Rassekh, Gabriella S. S. Groeneweg, Michelle Higginson, Kathy Li, Fudan Miao, Derek Yau, Donna Johnston, Mounira Ibrahim, Maja Krajinovic, Thai Hoa Tran, Jessica Tewfik, Jean‐François Bussières, Thaïna‐Rafi Jean‐Baptiste, Denis Lebel, Lucie Pecheux, Bina Gyawali, Gregory Guilcher, Gesche Riabowol, Geert ’t Jong, Michelle Staub, Geoff Cuvelier, Kathleen Felton, Sara Khalaj, Michael Rieder, Awatif Abuzgaia, Tamorah Lewis, Himal Ghimire, Paul Nathan, Kerry Goralski, Ketan Kulkarni, Zara Forbrigger, Colin J. D. Ross, Bruce C. Carleton   +39 more
wiley   +1 more source

Sequence permutations in the molecular evolution of DNA methyltransferases

BMC Evolutionary Biology, 2002
Background DNA methyltransferases (MTases), unlike MTases acting on other substrates, exhibit sequence permutation. Based on the sequential order of the cofactor-binding subdomain, the catalytic subdomain, and the target recognition domain (TRD), several
Bujnicki Janusz M
doaj   +1 more source

Language Time Series Analysis [PDF]

, 2006
We use the Detrended Fluctuation Analysis (DFA) and the Grassberger-Proccacia analysis (GP) methods in order to study language characteristics. Despite that we construct our signals using only word lengths or word frequencies, excluding in this way huge ...
Abrams, Alkiviadis Kalampokis, Amaral, Amit, Arneodo, Buldyrev, Buldyrev, Bunde, Cancho, de Oliveira, Grassberger, Grassberger, Havlin, Ivanov, Kantelhardt, Karakotsou, Koliopanos, Koscielny-Bunde, Kosmas Kosmidis, Kosmidis, Kotsavasiloglou, Mantegna, Mira, Montemurro, Nicolis, Nowak, Panos Argyrakis, Patriarca, Peng, Peng, Peng, Peng, Peng, Pollock, Schenkel, Schulze, Schulze, Schwammle, Thurner, Voss, Voss, Zipf   +41 more
core   +2 more sources

Optimization of DNA shuffling for high fidelity recombination [PDF]

Nucleic Acids Research, 1997
A convenient 'DNA shuffling' protocol for random recombination of homologous genes in vitro with a very low rate of associated point mutagenesis (0.05%) is described. In addition, the mutagenesis rate can be controlled over a wide range by the inclusion of Mn2+or Mg2+during DNase I digestion, by choice of DNA polymerase used during gene reassembly as ...
Zhao, Huimin, Arnold, Frances H.
openaire   +3 more sources

Mapping the evolution of mitochondrial complex I through structural variation

FEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin, Tingting Chen, James A. Letts   +2 more
wiley   +1 more source