Application of GelGreen™ in Cesium Chloride Density Gradients for DNA-Stable Isotope Probing Experiments. [PDF]
PLoS ONE, 2017 In this study, GelGreen™ was investigated as a replacement for SYBR® Safe to stain DNA in cesium chloride (CsCl) density gradients for DNA-stable isotope probing (SIP) experiments.
Jingfeng Gao +6 more
doaj +3 more sources
DNA ‐stable isotope probing DNA‐stable isotope probing (DNA‐SIP) identifies marine sponge‐associated bacteria actively utilizing dissolved organic matter (DOM) [PDF]
Environ Microbiol, 2021 Sponges possess exceptionally diverse associated microbial communities and play a major role in (re)cycling of dissolved organic matter (DOM) in marine ecosystems.
de Goeij, J.M. +13 more
core +2 more sources
DNA stable isotope probing reveals hidden microbial carbon processing pathways in agricultural soils [PDF]
Applied and Environmental MicrobiologyAgricultural soil management plays a key role in driving the microbial communities responsible for soil carbon cycling, yet mechanistic understanding of these effects has been limited by the inability to directly observe microbial activity in situ ...
Malak M. Tfaily
doaj +2 more sources
Virome DNA stable isotope probing reveals diverse active soil virus communities across ecosystem contexts [PDF]
mSystemsViruses are increasingly recognized as important players in soil ecosystems, but the active lytic virus populations that influence microbe-mediated terrestrial ecosystem processes remain mostly uncharacterized.
Ernest D. Osburn, Minjae Kim
doaj +2 more sources
Ammonium-Generating Microbial Consortia in Paddy Soil Revealed by DNA-Stable Isotope Probing and Metatranscriptomics [PDF]
MicroorganismsRice paddy fields are sustainable agricultural systems as soil microorganisms help maintain nitrogen fertility through generating ammonium. In these soils, dissimilatory nitrate reduction to ammonium (DNRA), nitrogen fixation, and denitrification are ...
Chao-Nan Wang, Yoko Masuda, Keishi Senoo
doaj +2 more sources
DNA-Stable Isotope Probing Shotgun Metagenomics Reveals the Resilience of Active Microbial Communities to Biochar Amendment in Oxisol Soil [PDF]
Frontiers in Microbiology, 2020 The functions and interactions of individual microbial populations and their genes in agricultural soils amended with biochar remain elusive but are crucial for a deeper understanding of nutrient cycling and carbon (C) sequestration.
Julian Yu +9 more
doaj +2 more sources
DNA stable‐isotope probing reveals potential key players for microbial decomposition and degradation of diatom‐derived marine particulate matter [PDF]
MicrobiologyOpen, 2020 Microbially mediated decomposition of particulate organic carbon (POC) is a central component of the oceanic carbon cycle, controlling the flux of organic carbon from the surface ocean to the deep ocean.
Ying Liu +5 more
doaj +2 more sources
DNA stable isotope probing reveals the impact of trophic interactions on bioaugmentation of soils with different pollution histories [PDF]
MicrobiomeBackground Bioaugmentation is considered a sustainable and cost-effective methodology to recover contaminated environments, but its outcome is highly variable.
Esteban E. Nieto +6 more
doaj +2 more sources
Integrated 13C-DNA Stable Isotope Probing and Metagenomics Approaches to Identify Bisphenol A Assimilating Microorganisms and Metabolic Pathways in Biofilms [PDF]
ToxicsBisphenol A (BPA) is a persistent environmental contaminant requiring effective removal strategies. Biofilms offer advantages over conventional activated sludge for refractory compound degradation, yet the specific microorganisms and mechanisms driving ...
Di Wang +6 more
doaj +2 more sources
Frontiers in Microbiology, 2022 Autotoxins secreted by roots into the soil can trigger rhizosphere microecological imbalances and affect root secretory properties resulting in conditions such as replanting disease.
Qianwen Liu +6 more
doaj +1 more source