Results 21 to 30 of about 35,316 (244)
Sequencing double-stranded DNA by strand displacement [PDF]
Nucleic Acids Research, 1997 Duplex probes with five base single-stranded overhangs can capture dsDNA targets from type IIS restriction nuclease digests. Ligation generates a predesigned nick site, where DNA polymerase can generate sequencing ladders by strand displacement or nick translation in the presence of trace amounts of dideoxynucleotides.
D J, Fu +3 more
openaire +2 more sources
Scalable DNA recognition circuits based on DNA strand displacement. [PDF]
Nanoscale AdvA scalable recognition circuit was designed and implemented using DNA and eventually a molecular comparator was constructed.
Wang F +5 more
europepmc +3 more sources
The Effect of Basepair Mismatch on DNA Strand Displacement. [PDF]
Biophys J, 2016 DNA strand displacement is a key reaction in DNA homologous recombination and DNA mismatch repair and is also heavily utilized in DNA-based computation and locomotion. Despite its ubiquity in science and engineering, sequence-dependent effects of displacement kinetics have not been extensively characterized.
Broadwater DWB, Kim HD.
europepmc +5 more sources
Rapid Visual Authentication Based on DNA Strand Displacement [PDF]
ACS Applied Materials & Interfaces, 2021 Novel ways to track and verify items of a high value or security is an ever-present need. Taggants made from deoxyribonucleic acid (DNA) have several advantageous properties, such as high information density and robust synthesis; however, existing methods require laboratory techniques to verify, limiting applications.
Kimberly L. Berk +11 more
openaire +2 more sources
Leakless DNA Strand Displacement Systems [PDF]
, 2015 While current experimental demonstrations have been limited to small computational tasks, DNA strand displacement systems DSD systemsi¾?[25] hold promise for sophisticated information processing within chemical or biological environments. A DSD system encodes designed reactions that are facilitated by three-way or four-way toehold-mediated strand ...
Chris Thachuk +2 more
openaire +2 more sources
G3: Genes, Genomes, Genetics, 2021 During lagging-strand synthesis, strand-displacement synthesis by DNA polymerase delta (Pol ∂), coupled to nucleolytic cleavage of DNA flap structures, produces a nick-translation reaction that replaces the DNA at the 5′ end of the preceding Okazaki ...
Natasha C Koussa, Duncan J Smith
doaj +1 more source
DNA Logic Multiplexing Using Toehold-Mediated Strand Displacement
IEEE Access, 2020 Since the discovery of the DNA Strand Displacement mechanism, researchers have implemented a lot of applications, such as DNA computing, DNA Circuits, Logic gates, and Chemical Reaction network.
Congzhou Chen +4 more
doaj +1 more source
BMC Molecular and Cell Biology, 2019 Background The discovery of thermostable DNA polymerases such as Taq DNA polymerase revolutionized amplification of DNA by polymerase chain reaction methods that rely on thermal cycling for strand separation.
Yvonne Piotrowski +2 more
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Nucleic acid strand displacement – from DNA nanotechnology to translational regulation
RNA Biology, 2023 Nucleic acid strand displacement reactions involve the competition of two or more DNA or RNA strands of similar sequence for binding to a complementary strand, and facilitate the isothermal replacement of an incumbent strand by an invader.
Friedrich C. Simmel
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A Novel Computational Method to Reduce Leaky Reaction in DNA Strand Displacement
Journal of Analytical Methods in Chemistry, 2015 DNA strand displacement technique is widely used in DNA programming, DNA biosensors, and gene analysis. In DNA strand displacement, leaky reactions can cause DNA signals decay and detecting DNA signals fails.
Xin Li +5 more
doaj +1 more source