Results 31 to 40 of about 2,195,182 (275)

Digital DNA Strand Displacement for Molecular Computing [PDF]

, 2023
Multiplexed analysis of feature biomarkers enhances the accuracy of molecular diagnostic results. Molecular computing systems exhibited potentials for intelligent analysis of multiplexed biomarkers.
Xin, Su, Jiayu, Yang, Yumin, Yan, Hongyang, Zhao, Xinmiao, Kang, Jiong, Huang, Huixiao, Yang, Linghao, Zhang   +7 more
core   +2 more sources

Sequencing double-stranded DNA by strand displacement [PDF]

Nucleic Acids Research, 1997
Duplex probes with five base single-stranded overhangs can capture dsDNA targets from type IIS restriction nuclease digests. Ligation generates a predesigned nick site, where DNA polymerase can generate sequencing ladders by strand displacement or nick translation in the presence of trace amounts of dideoxynucleotides.
D J, Fu, H, Köster, C L, Smith, C R, Cantor   +3 more
openaire   +2 more sources

Four-Analog Computation Based on DNA Strand Displacement [PDF]

ACS Omega, 2017
Chengye Zou, Xiaopeng Wei, Qiang Zhang, Chanjuan Liu, Changjun Zhou, Yuan Liu   +5 more
doaj   +2 more sources

Rapid Visual Authentication Based on DNA Strand Displacement [PDF]

ACS Applied Materials & Interfaces, 2021
Novel ways to track and verify items of a high value or security is an ever-present need. Taggants made from deoxyribonucleic acid (DNA) have several advantageous properties, such as high information density and robust synthesis; however, existing methods require laboratory techniques to verify, limiting applications.
Kimberly L. Berk, Steven M. Blum, Vanessa L. Funk, Yuhua Sun, In-Young Yang, Mark V. Gostomski, Pierce A. Roth, Alvin T. Liem, Peter A. Emanuel, Michael E. Hogan, Aleksandr E. Miklos, Matthew W. Lux   +11 more
openaire   +2 more sources

Characterization and engineering of a DNA polymerase reveals a single amino-acid substitution in the fingers subdomain to increase strand-displacement activity of A-family prokaryotic DNA polymerases

BMC Molecular and Cell Biology, 2019
Background The discovery of thermostable DNA polymerases such as Taq DNA polymerase revolutionized amplification of DNA by polymerase chain reaction methods that rely on thermal cycling for strand separation.
Yvonne Piotrowski, Man Kumari Gurung, Atle Noralf Larsen   +2 more
doaj   +1 more source

Nucleic acid strand displacement – from DNA nanotechnology to translational regulation

RNA Biology, 2023
Nucleic acid strand displacement reactions involve the competition of two or more DNA or RNA strands of similar sequence for binding to a complementary strand, and facilitate the isothermal replacement of an incumbent strand by an invader.
Friedrich C. Simmel
doaj   +1 more source

Single-molecule dilution and multiple displacement amplification for molecular haplotyping

BioTechniques, 2005
Separate haploid analysis is frequently required for heterozygous genotyping to resolve phase ambiguity or confirm allelic sequence. We demonstrate a technique of single-molecule dilution followed by multiple strand displacement amplification to ...
Philip Paul, Josh Apgar
doaj   +1 more source

Strong sequence dependence in RNA/DNA hybrid strand displacement kinetics

, 2023
Strand displacement reactions underlie dynamic nucleic acid nanotechnology. The kinetic and thermodynamic features of DNA-based displacement reactions are well understood and well predicted by current computational models.
Smith, Francesca G, Stevens, Molly M, Goertz, John P, Ouldridge, Thomas E   +3 more
core   +1 more source

Strand displacement synthesis by yeast DNA polymerase ε [PDF]

Nucleic Acids Research, 2016
DNA polymerase ε (Pol ε) is a replicative DNA polymerase with an associated 3'-5' exonuclease activity. Here, we explored the capacity of Pol ε to perform strand displacement synthesis, a process that influences many DNA transactions in vivo We found that Pol ε is unable to carry out extended strand displacement synthesis unless its 3'-5' exonuclease ...
Ganai, Rais A, Zhang, Xiao-Ping, Heyer, Wolf-Dietrich, Johansson, Erik   +3 more
openaire   +5 more sources

Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA [PDF]

Scientific Reports, 2015
Single nucleotide polymorphisms (SNPs) are a prime source of genetic diversity. Discriminating between different SNPs provides an enormous leap towards the better understanding of the uniqueness of biological systems. Here we report on a new approach for SNP discrimination using toehold-mediated DNA strand displacement.
Khodakov, D., Khodakova, A., Huang, D., Linacre, A., Ellis, A.   +4 more
openaire   +3 more sources