Results 31 to 40 of about 35,316 (244)

Single-molecule dilution and multiple displacement amplification for molecular haplotyping

BioTechniques, 2005
Separate haploid analysis is frequently required for heterozygous genotyping to resolve phase ambiguity or confirm allelic sequence. We demonstrate a technique of single-molecule dilution followed by multiple strand displacement amplification to ...
Philip Paul, Josh Apgar
doaj   +1 more source

Strand displacement synthesis by yeast DNA polymerase ε [PDF]

Nucleic Acids Research, 2016
DNA polymerase ε (Pol ε) is a replicative DNA polymerase with an associated 3'-5' exonuclease activity. Here, we explored the capacity of Pol ε to perform strand displacement synthesis, a process that influences many DNA transactions in vivo We found that Pol ε is unable to carry out extended strand displacement synthesis unless its 3'-5' exonuclease ...
Ganai, Rais A, Zhang, Xiao-Ping, Heyer, Wolf-Dietrich, Johansson, Erik   +3 more
openaire   +5 more sources

DNA dynamics and computation based on toehold-free strand displacement

Nature Communications, 2021
Synthetic DNA constructs can to used to recognise and respond to input signals. Here the authors present complex DNA nanostructures with toehold-free strand displacement for generation of ON/OFF switches and Boolean gates.
Hong Kang, Tong Lin, Xiaojin Xu, Qing-Shan Jia, Richard Lakerveld, Bryan Wei   +5 more
doaj   +1 more source

Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA [PDF]

Scientific Reports, 2015
Single nucleotide polymorphisms (SNPs) are a prime source of genetic diversity. Discriminating between different SNPs provides an enormous leap towards the better understanding of the uniqueness of biological systems. Here we report on a new approach for SNP discrimination using toehold-mediated DNA strand displacement.
Khodakov, D., Khodakova, A., Huang, D., Linacre, A., Ellis, A.   +4 more
openaire   +3 more sources

A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification

BioTechniques, 2014
The sensitivity and robustness of various DNA detection and amplification techniques are to a large extent determined by the properties of the DNA polymerase used.
Konstantin B. Ignatov, Ekaterina V. Barsova, Arkady F. Fradkov, Konstantin A. Blagodatskikh, Tatiana V. Kramarova, Vladimir M. Kramarov   +5 more
doaj   +1 more source

A Double-Layer Image Encryption Scheme Based on Chaotic Maps and DNA Strand Displacement

Journal of Chemistry, 2022
The image encryption schemes combining chaotic maps, DNA coding, and DNA sequence operation can effectively protect the image. In this paper, a double-layer image encryption scheme is proposed by combining chaotic maps with DNA strand displacement (DSD).
Zhen Tang, Zhixiang Yin, Risheng Wang, Xiyuan Wang, Jing Yang, Jianzhong Cui   +5 more
doaj   +1 more source

Highly sensitive electrochemical assay based on strand displacement polymerization-assisted CRISPR/Cas12a collateral cleavage

Electrochemistry Communications, 2023
In this work, a novel strand displacement polymerization strategy is designed with a single hairpin-structured DNA probe acting as both of primer and template.
Yulin Zhu, Xifeng Chen, Jiayue Shi, Haixuan Sun, Hua Chai, Peng Miao   +5 more
doaj   +1 more source

Design of DNA strand displacement reactions

Current Opinion in Biotechnology
DNA strand displacement (SD) reactions are central to the operation of many synthetic nucleic acid systems, including molecular circuits, sensors, and machines. Over the years, a broad set of design frameworks has emerged to accommodate various functional goals, initial configurations, and environmental conditions. Nevertheless, key challenges persist,
Jurinović, Križan, Mitra, Merry, Mukherjee, Rakesh, Ouldridge, Thomas E   +3 more
openaire   +3 more sources

DNA strand displacement, strand annealing and strand swapping by the Drosophila Bloom's syndrome helicase [PDF]

Nucleic Acids Research, 2007
Genetic analysis of the Drosophila Bloom's syndrome helicase homolog (mus309/DmBLM) indicates that DmBLM is required for the synthesis-dependent strand annealing (SDSA) pathway of homologous recombination. Here we report the first biochemical study of DmBLM.
Weinert, Brian T, Rio, Donald C
openaire   +4 more sources

In situ reduction of gold nanoparticles-decorated MXenes-based electrochemical sensing platform for KRAS gene detection

Frontiers in Bioengineering and Biotechnology, 2023
In this work, gold nanoparticles@Ti3C2 MXenes nanocomposites with excellent properties were combined with toehold-mediated DNA strand displacement reaction to construct an electrochemical circulating tumor DNA biosensor.
Xiongtao Yu, Silan Bai, Lishi Wang
doaj   +1 more source